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Protein

Isoamylase

Gene

iam

Organism
Flavobacterium sp.
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has a high rate of hydrolysis for glycogen. Does not cleave pullulan.

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

pH dependencei

Optimum pH is 6-7.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi162CalciumBy similarity1
Metal bindingi263CalciumBy similarity1
Metal bindingi264Calcium; via carbonyl oxygenBy similarity1
Metal bindingi266CalciumBy similarity1
Metal bindingi293CalciumBy similarity1
Active sitei410NucleophileBy similarity1
Active sitei458Proton donorBy similarity1
Sitei533Transition state stabilizerBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoamylase (EC:3.2.1.68)
Gene namesi
Name:iam
OrganismiFlavobacterium sp.
Taxonomic identifieri239 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000000142333 – 777IsoamylaseAdd BLAST745

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi419 ↔ 423By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO32611.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO32611.
SMRiO32611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O32611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPHAPQRQR SGQRLRALAL AALACALSPA HAAIDAQQLG ARYDAAQANL
60 70 80 90 100
AFRVYSSRAT RVEVFLYKNP TGSQEVARLA LSKDPATQVW SLSLPTSTIK
110 120 130 140 150
NTYGITGAVY YGYRAWGPNW PYDAAWTKGS ATGFVSDVDN AGNRFNPNKL
160 170 180 190 200
LLDPYAREIS QDPNTATCAD GTIYATGAAH RNKDSGLCAS KGIALAADAT
210 220 230 240 250
SVGSKPTRAL KDEVIYEVHV RGLTRNDDSV PAAERGTYKG AARKAAALAA
260 270 280 290 300
LGVTAVEFLP VQETQNDQND VDPNSTAGDN YWGYMTLNYF APDRRYAYDK
310 320 330 340 350
SAGGPTREWK AMVKAFHDAG IKVYIDVVYN HTGEGGPWSG TDGLSVYNLL
360 370 380 390 400
SFRGLDNPAY YSLSSDYKYP WDNTGVGGNY NTRHPIAQNL IVDSLAYWRD
410 420 430 440 450
ALGVDGFRFD LASVLGNSCQ HGCFNFDKND SGNALNRIVA ELPPRPAAGG
460 470 480 490 500
AGADLIAEPW AIGGNSYQVG GFPAGWAEWN GLYRDALRKK QNKLGVETVT
510 520 530 540 550
PGTLATRFAG SNDLYGDDGR KPWHSINFVV AHDGFTLNDL YAYNDKQNNQ
560 570 580 590 600
PWPYGPSDGG EDHNLSWNQG GIVAEQRKAA RTGLALLMLS AGVPMITGGD
610 620 630 640 650
EALRTQFGNN NTYNLDSAAN WLYWSRSALE ADHETYTKRL IAFRKAHPAL
660 670 680 690 700
RPANFYSASD TNGNVMEQLR WFKPDGAQAD SAYFNGADNH ALAWRIDGSE
710 720 730 740 750
FGDSASAIYV AYNGWSGAVD FKLPWPGTGK QWYRVTDTAT WNEGPNAVAL
760 770
PGSETLIGGE NTVYGMQARS LLLLIAK
Length:777
Mass (Da):84,340
Last modified:January 1, 1998 - v1
Checksum:i90548988EFD1445B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90120 Genomic DNA. Translation: AAB63356.1.

Similar proteinsi

Entry informationi

Entry nameiISOA_FLASP
AccessioniPrimary (citable) accession number: O32611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 10, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families