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Protein

Serine protease EspP

Gene

espP

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease with cytotoxic effect. Disrupts actin cytoskeleton resulting cell detachment in vitro.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Charge relay systemPROSITE-ProRule annotation
Active sitei156 – 1561Charge relay systemPROSITE-ProRule annotation
Active sitei263 – 2631Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiN04.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease EspP (EC:3.4.21.-)
Cleaved into the following 2 chains:
Alternative name(s):
Extracellular serine protease plasmid-encoded EspP
Gene namesi
Name:espP
Synonyms:pssA
Encoded oniPlasmid unnamed1 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Autotransporter protein EspP translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 55551 PublicationAdd
BLAST
Chaini56 – 13001245Serine protease EspPPRO_0000387593Add
BLAST
Chaini56 – 1023968Secreted autotransporter protein EspPPRO_0000042018Add
BLAST
Chaini1024 – 1300277Autotransporter protein EspP translocatorBy similarityPRO_0000042019Add
BLAST

Post-translational modificationi

Cleaved to release the mature protein from the outer membrane.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1023 – 10242CleavageBy similarity

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiO32591.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
acpPP0A6A82EBI-852989,EBI-542566From a different organism.
ybgCP0A8Z32EBI-852989,EBI-543276From a different organism.

Protein-protein interaction databases

IntActiO32591. 3 interactions.
STRINGi155864.L7020.

Structurei

3D structure databases

ProteinModelPortaliO32591.
SMRiO32591. Positions 1024-1300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 311255Peptidase S6PROSITE-ProRule annotationAdd
BLAST
Domaini1034 – 1300267AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105I1J. Bacteria.
COG3468. LUCA.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O32591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIYSLKYS HITGGLIAVS ELSGRVSSRA TGKKKHKRIL ALCFLGLLQS
60 70 80 90 100
SYSFASQMDI SNFYIRDYMD FAQNKGIFQA GATNIEIVKK DGSTLKLPEV
110 120 130 140 150
PFPDFSPVAN KGSTTSIGGA YSITATHNTK NHHSVATQNW GNSTYKQTDW
160 170 180 190 200
NTSHPDFAVS RLDKFVVETR GATEGADISL SKQQALERYG VNYKGEKKLI
210 220 230 240 250
AFRAGSGVVS VKKNGRITPF NEVSYKPEML NGSFVHIDDW SGWLILTNNQ
260 270 280 290 300
FDEFNNIASQ GDSGSALFVY DNQKKKWVVA GTVWGIYNYA NGKNHAAYSK
310 320 330 340 350
WNQTTIDNLK NKYSYNVDMS GAQVATIENG KLTGTGSDTT DIKNKDLIFT
360 370 380 390 400
GGGDILLKSS FDNGAGGLVF NDKKTYRVNG DDFTFKGAGV DTRNGSTVEW
410 420 430 440 450
NIRYDNKDNL HKIGDGTLDV RKTQNTNLKT GEGLVILGAE KTFNNIYITS
460 470 480 490 500
GDGTVRLNAE NALSGGEYNG IFFAKNGGTL DLNGYNQSFN KIAATDSGAV
510 520 530 540 550
ITNTSTKKSI LSLNNTADYI YHGNINGNLD VLQHHETKKE NRRLILDGGV
560 570 580 590 600
DTTNDISLRN TQLSMQGHAT EHAIYRDGAF SCSLPAPMRF LCGSDYVAGM
610 620 630 640 650
QNTEADAVKQ NGNAYKTNNA VSDLSQPDWE TGTFRFGTLH LENSDFSVGR
660 670 680 690 700
NANVIGDIQA SKSNITIGDT TAYIDLHAGK NITGDGFGFR QNIVRGNSQG
710 720 730 740 750
ETLFTGGITA EDSTIVIKDK AKALFSNYVY LLNTKATIEN GADVTTQSGM
760 770 780 790 800
FSTSDISISG NLSMTGNPDK DNKFEPSIYL NDASYLLTDD SARLVAKNKA
810 820 830 840 850
SVVGDIHSTK SASIMFGHDE SDLSQLSDRT SKGLALGLLG GFDVSYRGSV
860 870 880 890 900
NAPSASATMN NTWWQLTGDS ALKTLKSTNS MVYFTDSANN KKFHTLTVDE
910 920 930 940 950
LATSNSAYAM RTNLSESDKL EVKKHLSGEN NILLVDFLQK PTPEKQLNIE
960 970 980 990 1000
LVSAPKDTNE NVFKASKQTI GFSDVTPVIT TRETDDKITW SLTGYNTVAN
1010 1020 1030 1040 1050
KEATRNAAAL FSVDYKAFLN EVNNLNKRMG DLRDINGEAG AWARIMSGTG
1060 1070 1080 1090 1100
SASGGFSDNY THVQVGVDKK HELDGLDLFT GFTVTHTDSS ASADVFSGKT
1110 1120 1130 1140 1150
KSVGAGLYAS AMVDSGAYID LIGKYVHHDN EYTATFAGLG TRDYSTHSWY
1160 1170 1180 1190 1200
AGAEAGYRYH VTEDAWIEPQ AELVYGSVSG KQFAWKDQGM HLSMKDKDYN
1210 1220 1230 1240 1250
PLIGRTGVDV GKSFSGKDWK VTARAGLGYQ FDLLANGETV LRDASGEKRI
1260 1270 1280 1290 1300
KGEKDSRMLM SVGLNAEIRD NVRFGLEFEK SAFGKYNVDN AVNANFRYSF
Length:1,300
Mass (Da):141,710
Last modified:January 1, 1998 - v1
Checksum:iF84D24086DE77C24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13614 Genomic DNA. Translation: CAA73935.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13614 Genomic DNA. Translation: CAA73935.1.

3D structure databases

ProteinModelPortaliO32591.
SMRiO32591. Positions 1024-1300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO32591. 3 interactions.
STRINGi155864.L7020.

Protein family/group databases

MEROPSiN04.002.

Proteomic databases

PRIDEiO32591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105I1J. Bacteria.
COG3468. LUCA.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESPP_ECOLX
AccessioniPrimary (citable) accession number: O32591
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded on an unnamed 94 kb plasmid.1 Publication

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.