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Protein

Serine protease EspP

Gene

espP

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease with cytotoxic effect. Disrupts actin cytoskeleton resulting cell detachment in vitro.1 Publication

Miscellaneous

Encoded on an unnamed 94 kb plasmid.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127Charge relay systemPROSITE-ProRule annotation1
Active sitei156Charge relay systemPROSITE-ProRule annotation1
Active sitei263Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processVirulence

Protein family/group databases

MEROPSiN04.002

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease EspP (EC:3.4.21.-)
Cleaved into the following 2 chains:
Alternative name(s):
Extracellular serine protease plasmid-encoded EspP
Gene namesi
Name:espP
Synonyms:pssA
Encoded oniPlasmid unnamed1 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

Autotransporter protein EspP translocator :

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 551 PublicationAdd BLAST55
ChainiPRO_000038759356 – 1300Serine protease EspPAdd BLAST1245
ChainiPRO_000004201856 – 1023Secreted autotransporter protein EspPAdd BLAST968
ChainiPRO_00000420191024 – 1300Autotransporter protein EspP translocatorBy similarityAdd BLAST277

Post-translational modificationi

Cleaved to release the mature protein from the outer membrane.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1023 – 1024CleavageBy similarity2

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiO32591

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ybgCP0A8Z33EBI-852989,EBI-543276From Escherichia coli (strain K12).

Protein-protein interaction databases

IntActiO32591, 3 interactors

Structurei

3D structure databases

ProteinModelPortaliO32591
SMRiO32591
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 311Peptidase S6PROSITE-ProRule annotationAdd BLAST255
Domaini1034 – 1300AutotransporterPROSITE-ProRule annotationAdd BLAST267

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105I1J Bacteria
COG3468 LUCA

Family and domain databases

Gene3Di2.160.20.20, 2 hits
InterProiView protein in InterPro
IPR005546 Autotransporte_beta
IPR036709 Autotransporte_beta_dom_sf
IPR024973 ESPR
IPR006315 OM_autotransptr_brl
IPR012332 P22_tailspike-like_C_sf
IPR011050 Pectin_lyase_fold/virulence
IPR000710 Peptidase_S6
IPR030396 Peptidase_S6_dom
PfamiView protein in Pfam
PF03797 Autotransporter, 1 hit
PF13018 ESPR, 1 hit
PF02395 Peptidase_S6, 1 hit
PRINTSiPR00921 IGASERPTASE
SMARTiView protein in SMART
SM00869 Autotransporter, 1 hit
SUPFAMiSSF103515 SSF103515, 1 hit
SSF51126 SSF51126, 2 hits
TIGRFAMsiTIGR01414 autotrans_barl, 1 hit
PROSITEiView protein in PROSITE
PS51208 AUTOTRANSPORTER, 1 hit
PS51691 PEPTIDASE_S6, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O32591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIYSLKYS HITGGLIAVS ELSGRVSSRA TGKKKHKRIL ALCFLGLLQS
60 70 80 90 100
SYSFASQMDI SNFYIRDYMD FAQNKGIFQA GATNIEIVKK DGSTLKLPEV
110 120 130 140 150
PFPDFSPVAN KGSTTSIGGA YSITATHNTK NHHSVATQNW GNSTYKQTDW
160 170 180 190 200
NTSHPDFAVS RLDKFVVETR GATEGADISL SKQQALERYG VNYKGEKKLI
210 220 230 240 250
AFRAGSGVVS VKKNGRITPF NEVSYKPEML NGSFVHIDDW SGWLILTNNQ
260 270 280 290 300
FDEFNNIASQ GDSGSALFVY DNQKKKWVVA GTVWGIYNYA NGKNHAAYSK
310 320 330 340 350
WNQTTIDNLK NKYSYNVDMS GAQVATIENG KLTGTGSDTT DIKNKDLIFT
360 370 380 390 400
GGGDILLKSS FDNGAGGLVF NDKKTYRVNG DDFTFKGAGV DTRNGSTVEW
410 420 430 440 450
NIRYDNKDNL HKIGDGTLDV RKTQNTNLKT GEGLVILGAE KTFNNIYITS
460 470 480 490 500
GDGTVRLNAE NALSGGEYNG IFFAKNGGTL DLNGYNQSFN KIAATDSGAV
510 520 530 540 550
ITNTSTKKSI LSLNNTADYI YHGNINGNLD VLQHHETKKE NRRLILDGGV
560 570 580 590 600
DTTNDISLRN TQLSMQGHAT EHAIYRDGAF SCSLPAPMRF LCGSDYVAGM
610 620 630 640 650
QNTEADAVKQ NGNAYKTNNA VSDLSQPDWE TGTFRFGTLH LENSDFSVGR
660 670 680 690 700
NANVIGDIQA SKSNITIGDT TAYIDLHAGK NITGDGFGFR QNIVRGNSQG
710 720 730 740 750
ETLFTGGITA EDSTIVIKDK AKALFSNYVY LLNTKATIEN GADVTTQSGM
760 770 780 790 800
FSTSDISISG NLSMTGNPDK DNKFEPSIYL NDASYLLTDD SARLVAKNKA
810 820 830 840 850
SVVGDIHSTK SASIMFGHDE SDLSQLSDRT SKGLALGLLG GFDVSYRGSV
860 870 880 890 900
NAPSASATMN NTWWQLTGDS ALKTLKSTNS MVYFTDSANN KKFHTLTVDE
910 920 930 940 950
LATSNSAYAM RTNLSESDKL EVKKHLSGEN NILLVDFLQK PTPEKQLNIE
960 970 980 990 1000
LVSAPKDTNE NVFKASKQTI GFSDVTPVIT TRETDDKITW SLTGYNTVAN
1010 1020 1030 1040 1050
KEATRNAAAL FSVDYKAFLN EVNNLNKRMG DLRDINGEAG AWARIMSGTG
1060 1070 1080 1090 1100
SASGGFSDNY THVQVGVDKK HELDGLDLFT GFTVTHTDSS ASADVFSGKT
1110 1120 1130 1140 1150
KSVGAGLYAS AMVDSGAYID LIGKYVHHDN EYTATFAGLG TRDYSTHSWY
1160 1170 1180 1190 1200
AGAEAGYRYH VTEDAWIEPQ AELVYGSVSG KQFAWKDQGM HLSMKDKDYN
1210 1220 1230 1240 1250
PLIGRTGVDV GKSFSGKDWK VTARAGLGYQ FDLLANGETV LRDASGEKRI
1260 1270 1280 1290 1300
KGEKDSRMLM SVGLNAEIRD NVRFGLEFEK SAFGKYNVDN AVNANFRYSF
Length:1,300
Mass (Da):141,710
Last modified:January 1, 1998 - v1
Checksum:iF84D24086DE77C24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13614 Genomic DNA Translation: CAA73935.1

Similar proteinsi

Entry informationi

Entry nameiESPP_ECOLX
AccessioniPrimary (citable) accession number: O32591
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

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