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Protein

Sulfur carrier protein ThiS

Gene

thiS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is the sulfur donor in the synthesis of the thiazole phosphate moiety of thiamine phosphate.1 Publication

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesis
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THIS-MONOMER
MetaCyc:THIS-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfur carrier protein ThiS
Alternative name(s):
Thiamine biosynthesis protein ThiS
Gene namesi
Name:thiS
Synonyms:thiG1
Ordered Locus Names:b4407, JW3955
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14363 thiS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000725181 – 66Sulfur carrier protein ThiSAdd BLAST66

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei661-thioglycine; alternate1 Publication1
Modified residuei66Glycyl adenylate; alternate1 Publication1
Cross-linki66Glycyl cysteine dithioester (Gly-Cys) (interchain with C-184 in ThiF); alternate

Post-translational modificationi

C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) by ThiF, then thiocarboxylated (-COSH) by ThiI.1 Publication

Keywords - PTMi

Phosphoprotein, Thioester bond

Proteomic databases

EPDiO32583
PaxDbiO32583
PRIDEiO32583

Interactioni

Protein-protein interaction databases

BioGridi4261404, 4 interactors
IntActiO32583, 1 interactor
STRINGi316385.ECDH10B_4180

Structurei

Secondary structure

166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi8 – 10Combined sources3
Helixi18 – 25Combined sources8
Beta strandi32 – 36Combined sources5
Beta strandi39 – 41Combined sources3
Helixi43 – 45Combined sources3
Turni46 – 48Combined sources3
Beta strandi56 – 61Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F0ZNMR-A1-66[»]
1ZUDX-ray1.982/41-66[»]
ProteinModelPortaliO32583
SMRiO32583
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO32583

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfur carrier protein ThiS family.Curated

Phylogenomic databases

eggNOGiENOG4105WHD Bacteria
COG2104 LUCA
HOGENOMiHOG000248060
InParanoidiO32583
KOiK03154
OMAiMRILFND
PhylomeDBiO32583

Family and domain databases

Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR012675 Beta-grasp_dom_sf
IPR016155 Mopterin_synth/thiamin_S_b
IPR010035 Thi_S
IPR003749 ThiS/MoaD-like
PANTHERiPTHR34472 PTHR34472, 1 hit
PfamiView protein in Pfam
PF02597 ThiS, 1 hit
SUPFAMiSSF54285 SSF54285, 1 hit
TIGRFAMsiTIGR01683 thiS, 1 hit

Sequencei

Sequence statusi: Complete.

O32583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQILFNDQAM QCAAGQTVHE LLEQLDQRQA GAALAINQQI VPREQWAQHI
60
VQDGDQILLF QVIAGG
Length:66
Mass (Da):7,311
Last modified:January 1, 1998 - v1
Checksum:i9AB3C87835758B40
GO

Mass spectrometryi

Molecular mass is 7310.75 Da from positions 1 - 66. Determined by ESI. 1 Publication
Molecular mass is 7310.74 Da from positions 1 - 66. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88701 Genomic DNA Translation: AAB95620.1
U00006 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAT48237.1
AP009048 Genomic DNA Translation: BAE77328.1
PIRiS77700
RefSeqiWP_001166226.1, NZ_LN832404.1
YP_026279.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48237; AAT48237; b4407
BAE77328; BAE77328; BAE77328
GeneIDi2847702
KEGGiecj:JW3955
eco:b4407
PATRICifig|1411691.4.peg.2720

Similar proteinsi

Entry informationi

Entry nameiTHIS_ECOLI
AccessioniPrimary (citable) accession number: O32583
Secondary accession number(s): Q2M8S8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health