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Protein

Sulfur carrier protein ThiS

Gene

thiS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is the sulfur donor in the synthesis of the thiazole phosphate moiety of thiamine phosphate.1 Publication

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THIS-MONOMER.
ECOL316407:JW3955-MONOMER.
MetaCyc:THIS-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfur carrier protein ThiS
Alternative name(s):
Thiamine biosynthesis protein ThiS
Gene namesi
Name:thiS
Synonyms:thiG1
Ordered Locus Names:b4407, JW3955
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14363. thiS.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6666Sulfur carrier protein ThiSPRO_0000072518Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 6611-thioglycine; alternate1 Publication
Modified residuei66 – 661Glycyl adenylate; alternate1 Publication
Cross-linki66 – 66Glycyl cysteine dithioester (Gly-Cys) (interchain with C-184 in ThiF); alternate

Post-translational modificationi

C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) by ThiF, then thiocarboxylated (-COSH) by ThiI.1 Publication

Keywords - PTMi

Phosphoprotein, Thioester bond

Proteomic databases

EPDiO32583.
PaxDbiO32583.

Interactioni

Protein-protein interaction databases

BioGridi4261404. 4 interactions.
IntActiO32583. 1 interaction.
STRINGi511145.b4407.

Structurei

Secondary structure

1
66
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Beta strandi8 – 103Combined sources
Helixi18 – 258Combined sources
Beta strandi32 – 365Combined sources
Beta strandi39 – 413Combined sources
Helixi43 – 453Combined sources
Turni46 – 483Combined sources
Beta strandi56 – 616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F0ZNMR-A1-66[»]
1ZUDX-ray1.982/41-66[»]
ProteinModelPortaliO32583.
SMRiO32583. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO32583.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfur carrier protein ThiS family.Curated

Phylogenomic databases

eggNOGiENOG4105WHD. Bacteria.
COG2104. LUCA.
HOGENOMiHOG000248060.
InParanoidiO32583.
KOiK03154.
OMAiFNDEPLQ.
PhylomeDBiO32583.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR012675. Beta-grasp_dom.
IPR016155. Mopterin_synth/thiamin_S_b.
IPR010035. Thi_S.
IPR003749. ThiS/MoaD.
[Graphical view]
PfamiPF02597. ThiS. 1 hit.
[Graphical view]
SUPFAMiSSF54285. SSF54285. 1 hit.
TIGRFAMsiTIGR01683. thiS. 1 hit.

Sequencei

Sequence statusi: Complete.

O32583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQILFNDQAM QCAAGQTVHE LLEQLDQRQA GAALAINQQI VPREQWAQHI
60
VQDGDQILLF QVIAGG
Length:66
Mass (Da):7,311
Last modified:January 1, 1998 - v1
Checksum:i9AB3C87835758B40
GO

Mass spectrometryi

Molecular mass is 7310.75 Da from positions 1 - 66. Determined by ESI. 1 Publication
Molecular mass is 7310.74 Da from positions 1 - 66. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88701 Genomic DNA. Translation: AAB95620.1.
U00006 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAT48237.1.
AP009048 Genomic DNA. Translation: BAE77328.1.
PIRiS77700.
RefSeqiWP_001166226.1. NZ_LN832404.1.
YP_026279.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48237; AAT48237; b4407.
BAE77328; BAE77328; BAE77328.
GeneIDi2847702.
KEGGiecj:JW3955.
eco:b4407.
PATRICi32123505. VBIEscCol129921_4105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88701 Genomic DNA. Translation: AAB95620.1.
U00006 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAT48237.1.
AP009048 Genomic DNA. Translation: BAE77328.1.
PIRiS77700.
RefSeqiWP_001166226.1. NZ_LN832404.1.
YP_026279.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F0ZNMR-A1-66[»]
1ZUDX-ray1.982/41-66[»]
ProteinModelPortaliO32583.
SMRiO32583. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261404. 4 interactions.
IntActiO32583. 1 interaction.
STRINGi511145.b4407.

Proteomic databases

EPDiO32583.
PaxDbiO32583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48237; AAT48237; b4407.
BAE77328; BAE77328; BAE77328.
GeneIDi2847702.
KEGGiecj:JW3955.
eco:b4407.
PATRICi32123505. VBIEscCol129921_4105.

Organism-specific databases

EchoBASEiEB4108.
EcoGeneiEG14363. thiS.

Phylogenomic databases

eggNOGiENOG4105WHD. Bacteria.
COG2104. LUCA.
HOGENOMiHOG000248060.
InParanoidiO32583.
KOiK03154.
OMAiFNDEPLQ.
PhylomeDBiO32583.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciEcoCyc:THIS-MONOMER.
ECOL316407:JW3955-MONOMER.
MetaCyc:THIS-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO32583.
PROiO32583.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR012675. Beta-grasp_dom.
IPR016155. Mopterin_synth/thiamin_S_b.
IPR010035. Thi_S.
IPR003749. ThiS/MoaD.
[Graphical view]
PfamiPF02597. ThiS. 1 hit.
[Graphical view]
SUPFAMiSSF54285. SSF54285. 1 hit.
TIGRFAMsiTIGR01683. thiS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIS_ECOLI
AccessioniPrimary (citable) accession number: O32583
Secondary accession number(s): Q2M8S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.