Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sulfur carrier protein ThiS

Gene

thiS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is the sulfur donor in the synthesis of the thiazole phosphate moiety of thiamine phosphate.1 Publication

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:THIS-MONOMER.
ECOL316407:JW3955-MONOMER.
MetaCyc:THIS-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfur carrier protein ThiS
Alternative name(s):
Thiamine biosynthesis protein ThiS
Gene namesi
Name:thiS
Synonyms:thiG1
Ordered Locus Names:b4407, JW3955
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14363. thiS.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000725181 – 66Sulfur carrier protein ThiSAdd BLAST66

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei661-thioglycine; alternate1 Publication1
Modified residuei66Glycyl adenylate; alternate1 Publication1
Cross-linki66Glycyl cysteine dithioester (Gly-Cys) (interchain with C-184 in ThiF); alternate

Post-translational modificationi

C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) by ThiF, then thiocarboxylated (-COSH) by ThiI.1 Publication

Keywords - PTMi

Phosphoprotein, Thioester bond

Proteomic databases

EPDiO32583.
PaxDbiO32583.
PRIDEiO32583.

Interactioni

Protein-protein interaction databases

BioGridi4261404. 4 interactors.
IntActiO32583. 1 interactor.
STRINGi511145.b4407.

Structurei

Secondary structure

166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi8 – 10Combined sources3
Helixi18 – 25Combined sources8
Beta strandi32 – 36Combined sources5
Beta strandi39 – 41Combined sources3
Helixi43 – 45Combined sources3
Turni46 – 48Combined sources3
Beta strandi56 – 61Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F0ZNMR-A1-66[»]
1ZUDX-ray1.982/41-66[»]
ProteinModelPortaliO32583.
SMRiO32583.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO32583.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfur carrier protein ThiS family.Curated

Phylogenomic databases

eggNOGiENOG4105WHD. Bacteria.
COG2104. LUCA.
HOGENOMiHOG000248060.
InParanoidiO32583.
KOiK03154.
OMAiFNDEPLQ.
PhylomeDBiO32583.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR012675. Beta-grasp_dom.
IPR016155. Mopterin_synth/thiamin_S_b.
IPR010035. Thi_S.
IPR003749. ThiS/MoaD.
[Graphical view]
PfamiPF02597. ThiS. 1 hit.
[Graphical view]
SUPFAMiSSF54285. SSF54285. 1 hit.
TIGRFAMsiTIGR01683. thiS. 1 hit.

Sequencei

Sequence statusi: Complete.

O32583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQILFNDQAM QCAAGQTVHE LLEQLDQRQA GAALAINQQI VPREQWAQHI
60
VQDGDQILLF QVIAGG
Length:66
Mass (Da):7,311
Last modified:January 1, 1998 - v1
Checksum:i9AB3C87835758B40
GO

Mass spectrometryi

Molecular mass is 7310.75 Da from positions 1 - 66. Determined by ESI. 1 Publication
Molecular mass is 7310.74 Da from positions 1 - 66. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88701 Genomic DNA. Translation: AAB95620.1.
U00006 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAT48237.1.
AP009048 Genomic DNA. Translation: BAE77328.1.
PIRiS77700.
RefSeqiWP_001166226.1. NZ_LN832404.1.
YP_026279.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48237; AAT48237; b4407.
BAE77328; BAE77328; BAE77328.
GeneIDi2847702.
KEGGiecj:JW3955.
eco:b4407.
PATRICi32123505. VBIEscCol129921_4105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88701 Genomic DNA. Translation: AAB95620.1.
U00006 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAT48237.1.
AP009048 Genomic DNA. Translation: BAE77328.1.
PIRiS77700.
RefSeqiWP_001166226.1. NZ_LN832404.1.
YP_026279.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F0ZNMR-A1-66[»]
1ZUDX-ray1.982/41-66[»]
ProteinModelPortaliO32583.
SMRiO32583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261404. 4 interactors.
IntActiO32583. 1 interactor.
STRINGi511145.b4407.

Proteomic databases

EPDiO32583.
PaxDbiO32583.
PRIDEiO32583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48237; AAT48237; b4407.
BAE77328; BAE77328; BAE77328.
GeneIDi2847702.
KEGGiecj:JW3955.
eco:b4407.
PATRICi32123505. VBIEscCol129921_4105.

Organism-specific databases

EchoBASEiEB4108.
EcoGeneiEG14363. thiS.

Phylogenomic databases

eggNOGiENOG4105WHD. Bacteria.
COG2104. LUCA.
HOGENOMiHOG000248060.
InParanoidiO32583.
KOiK03154.
OMAiFNDEPLQ.
PhylomeDBiO32583.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciEcoCyc:THIS-MONOMER.
ECOL316407:JW3955-MONOMER.
MetaCyc:THIS-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO32583.
PROiO32583.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR012675. Beta-grasp_dom.
IPR016155. Mopterin_synth/thiamin_S_b.
IPR010035. Thi_S.
IPR003749. ThiS/MoaD.
[Graphical view]
PfamiPF02597. ThiS. 1 hit.
[Graphical view]
SUPFAMiSSF54285. SSF54285. 1 hit.
TIGRFAMsiTIGR01683. thiS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIS_ECOLI
AccessioniPrimary (citable) accession number: O32583
Secondary accession number(s): Q2M8S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.