Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

etpN

Organism
Escherichia coli
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue.UniRule annotation

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, MethyltransferaseUniRule annotation, ProteaseUniRule annotation, Transferase

Protein family/group databases

MEROPSiA24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzymeUniRule annotation (EC:2.1.1.-UniRule annotation, EC:3.4.23.43UniRule annotation)
Gene namesi
Name:etpNImported
Encoded oniPlasmid p0157Imported
Plasmid pSFO157Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei91 – 11020HelicalSequence analysisAdd
BLAST
Transmembranei116 – 13217HelicalSequence analysisAdd
BLAST
Transmembranei144 – 16219HelicalSequence analysisAdd
BLAST
Transmembranei182 – 20928HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Interactioni

Protein-protein interaction databases

STRINGi155864.L7043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 8876DiS_P_DiSInterPro annotationAdd
BLAST
Domaini99 – 206108Peptidase_A24InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A24 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
KOiK02464.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

O32576-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLERRWRREA LTLLAHSVPE SGPAFNLMIP QSHCPCCFHP LGIRDNIPLL
60 70 80 90 100
SYLFLKGKSR CCGERIPVYY PLVEITNSVL FILAASRFPP GLTLAAAWLF
110 120 130 140 150
ISMLLVLAVI DCHTALLPDV LTLPLLWLGL LFSLQRGVVT LEEAVVGAVS
160 170 180 190 200
GYLCLWGLYW LFRFATGREG LGYGDFKLAA ALGAWMGWQA LPSILLFASV
210 220 230
SGLIVTVLLR IVTATDFTRP LPFGPWLALS GVCHFLLM
Length:238
Mass (Da):26,287
Last modified:January 1, 1998 - v1
Checksum:i7E6732304EA4905D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF401292 Genomic DNA. Translation: AAR00423.1.
Y09824 Genomic DNA. Translation: CAA70965.1.
PIRiT00222.
RefSeqiWP_001358889.1. NC_009602.1.
YP_001294696.1. NC_009602.1.

Genome annotation databases

GeneIDi5290912.
KEGGipg:5290912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF401292 Genomic DNA. Translation: AAR00423.1.
Y09824 Genomic DNA. Translation: CAA70965.1.
PIRiT00222.
RefSeqiWP_001358889.1. NC_009602.1.
YP_001294696.1. NC_009602.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.L7043.

Protein family/group databases

MEROPSiA24.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5290912.
KEGGipg:5290912.

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
KOiK02464.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Publicationsi

  1. "A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strains."
    Schmidt H., Henkel B., Karch H.
    FEMS Microbiol. Lett. 148:265-272(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: EDL933Imported.
    Plasmid: p0157
  2. "Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H(-)."
    Brunder W., Khan A.S., Hacker J., Karch H.
    Infect. Immun. 69:4447-4457(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 3072/96Imported.
    Plasmid: pSFO157
  3. "Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96."
    Brunder W., Karch H., Schmidt H.
    Int. J. Med. Microbiol. 296:467-474(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 3072/96Imported.
    Plasmid: pSFO157

Entry informationi

Entry nameiO32576_ECOLX
AccessioniPrimary (citable) accession number: O32576
Entry historyi
Integrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Multifunctional enzymeUniRule annotation, PlasmidImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.