O32522 (PTHB_ERWAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucitol/sorbitol-specific phosphotransferase enzyme IIB component
EC=2.7.1.69

Alternative name(s):
EII-Gut
PTS system glucitol/sorbitol-specific EIIB component

Gene names

Name:

srlE

Organism

Erwinia amylovora (Fire blight bacteria)

Taxonomic identifier

552 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucitol/sorbitol transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-5 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Glucitol/sorbitol-specific phosphotransferase enzyme IIB component		PRO_0000186563
Regions
	Transmembrane	191 – 211	21	Helical; Potential
	Transmembrane	220 – 240	21	Helical; Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Transmembrane	271 – 291	21	Helical; Potential
	Transmembrane	304 – 324	21	Helical; Potential
	Domain	1 – 192	192	PTS EIIB type-5
	Region	193 – 333	141	Unknown
Sites
	Active site	72	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O32522 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6181206F04A61CAB	FASTA	333	34,292
	10 20 30 40 50 60 MANTIEIRKG ESGWGGPLSI NVTAGKKIVY ITAGTKPAIV DHLVALTGWE AVDGFKQGEP 70 80 90 100 110 120 PAEEIGVAVI DCGGTLRCGL YPKRRIPTIN IHATGKSGPL AQFITEDIYV SGVRVADIRV 130 140 150 160 170 180 ANDAEAAPPE VAVADVAVNA GKGTGRDYDT SKKITEQSDG LLAKVGMGMG SAVAILFQSG 190 200 210 220 230 240 RETIDTVLKT ILPFMAFVSA LIGIIMASGL GDFIAHGLTP LANSPVGLVT LALICSFPLL 250 260 270 280 290 300 SPFLGPGAVI AQVIGVLVGV QIGQGTIPPH LALPALFAIN AQAACDFIPV GLSLANARQE 310 320 330 TVRVGVPAVL VGRFITGAPT VLLAWAASSF IYH

References

[1]	"Genetics of sorbitol metabolism in Erwinia amylovora and its influence on bacterial virulence." Aldridge P., Metzger M., Geider K. Mol. Gen. Genet. 256:611-619(1997) [PubMed: 9435786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EA7/74.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y14603 Genomic DNA. Translation: CAA74942.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011638. PTS_EIIBC_GUT_C. IPR011618. PTS_EIIBC_GUT_N. IPR004702. PTS_sorb_EIIBC. [Graphical view]
Pfam	PF07663. EIIBC-GUT_C. 1 hit. PF03612. EIIBC-GUT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00825. EIIBC-GUT. 1 hit.
PROSITE	PS51102. PTS_EIIB_TYPE_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTHB_ERWAM

Accession

Primary (citable) accession number: O32522

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 31, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program