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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi145Zinc 1UniRule annotation1
Metal bindingi148Zinc 1UniRule annotation1
Metal bindingi162Zinc 2UniRule annotation1
Metal bindingi165Zinc 2UniRule annotation1
Metal bindingi184Zinc 2UniRule annotation1
Metal bindingi187Zinc 2UniRule annotation1
Metal bindingi198Zinc 1UniRule annotation1
Metal bindingi201Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri132 – 210CR-typeUniRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:BU152
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000707451 – 377Chaperone protein DnaJAdd BLAST377

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi107806.BU152.

Structurei

3D structure databases

ProteinModelPortaliO32465.
SMRiO32465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 70JUniRule annotationAdd BLAST66
Repeati145 – 152CXXCXGXG motif8
Repeati162 – 169CXXCXGXG motif8
Repeati184 – 191CXXCXGXG motif8
Repeati198 – 205CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 115Gly-richAdd BLAST39

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri132 – 210CR-typeUniRule annotationAdd BLAST79

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR036869. DnaJ_dom_sf.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

O32465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKDYYQIL GIPKSAEERE IKKAYKKLAM KYHPDRNQGD KTAEGKFKEI
60 70 80 90 100
KEAYEILINE EKRSAYDQYG HAAFENGQSN STYSTFTNSA DFSDIFGDVF
110 120 130 140 150
GDIFGGNRTQ RAKKGADLCY NMEITLEEAV KGIKKEIQIP TLQKCKTCYG
160 170 180 190 200
SGTRTGTKPR SCSTCHGKGQ IHIRKGFFTV QQSCPTCHGK GTIITDPCNL
210 220 230 240 250
CHGQGRVETY KILSVKIPPG LDTNDRIRLN NEGEAGANGA QSGDLYVQIT
260 270 280 290 300
VKKHPIFERE GNNLYCEVPI NFTMAALGGE IEVPTLDGRV KLKIPYETQS
310 320 330 340 350
GKLFRIRGRG VKSVQNRNQG DLLCRVVVET PVNLNEQQKN LLHELGNSFH
360 370
GFRGEKNSPR SKRFFDGVKR FFDDLTR
Length:377
Mass (Da):42,344
Last modified:December 1, 2000 - v2
Checksum:iF232B95530CC298B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185P → S in BAA21965 (PubMed:9276669).Curated1
Sequence conflicti270I → K in BAA21965 (PubMed:9276669).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88673 Genomic DNA. Translation: BAA21965.1.
BA000003 Genomic DNA. Translation: BAB12870.1.
PIRiJC5609.
RefSeqiNP_239984.1. NC_002528.1.
WP_009874108.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12870; BAB12870; BAB12870.
GeneIDi1109596.
KEGGibuc:BU152.
PATRICifig|107806.10.peg.162.

Similar proteinsi

Entry informationi

Entry nameiDNAJ_BUCAI
AccessioniPrimary (citable) accession number: O32465
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 1, 2000
Last modified: October 25, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families