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Protein

Chaperone protein DnaK

Gene

dnaK

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Acts as a chaperone.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-153-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaK
Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70
Gene namesi
Name:dnaK
Ordered Locus Names:BU153
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Chaperone protein DnaKPRO_0000078433Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991Phosphothreonine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO32464.

Expressioni

Inductioni

By stress conditions e.g. heat shock (By similarity).By similarity

Interactioni

Protein-protein interaction databases

STRINGi107806.BU153.

Structurei

3D structure databases

ProteinModelPortaliO32464.
SMRiO32464. Positions 1-605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228136.
KOiK04043.
OMAiFYSAKRF.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK. 1 hit.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKIIGIDLG TTNSCVAIMD GNKPRVLENA EGDRTTPSII AYTQEGEVLV
60 70 80 90 100
GQPAKRQAIT NPKNTLFAIK RLIGRKFKDD EVQRDIKIMP YNIVNSDNGD
110 120 130 140 150
AWIDVKKQKM APPQISAEVL KKMKKTAEDY LGETIKEAVI TVPAYFNDAQ
160 170 180 190 200
RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGKGNRTI AVYDLGGGTF
210 220 230 240 250
DISIIEIDEV DKEKTFEVLA TNGDTHLGGE DFDSRLINYL VTEFKKEQGI
260 270 280 290 300
DLRNDPLSMQ RLKESAEKAK IELSSAQQTD VNLPYITADS NGPKHLNIKV
310 320 330 340 350
TRAKLESLVE DLILRSIEPL KVALKDAGLS VTDINDVILV GGQTRMPMVQ
360 370 380 390 400
SKVADFFGKE PRKDVNPDEA VAVGAAVQGG VLSGDVKDVL LLDVTPLSLG
410 420 430 440 450
IETMGGIMTS LINKNTTIPT KHSQIFSTAE DNQSAVTIHV LQGERKRSSD
460 470 480 490 500
NKSLGQFNLD GINPAPRGTA QIEVTFDIDS DGILHVSAKD KKTGKEQKIT
510 520 530 540 550
IKASSGLNEE EIKKMVNDAE ANSEADQKFE ELIQTRNQGD QLVHSIKKQL
560 570 580 590 600
NENKNSIEEE SKKDIQLALN KLENALKGED KSDIEKNIQN LLKISSKLTE
610 620 630
INQKKSEKDQ KDNNMSANKK DENVVDAEFE EIKDPKK
Length:637
Mass (Da):70,042
Last modified:January 1, 1998 - v1
Checksum:i838C8C215A648BDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88673 Genomic DNA. Translation: BAA21964.1.
BA000003 Genomic DNA. Translation: BAB12871.1.
RefSeqiNP_239985.1. NC_002528.1.
WP_009874109.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12871; BAB12871; BAB12871.
GeneIDi1109597.
KEGGibuc:BU153.
PATRICi21243822. VBIBucAph127364_0163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88673 Genomic DNA. Translation: BAA21964.1.
BA000003 Genomic DNA. Translation: BAB12871.1.
RefSeqiNP_239985.1. NC_002528.1.
WP_009874109.1. NC_002528.1.

3D structure databases

ProteinModelPortaliO32464.
SMRiO32464. Positions 1-605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU153.

Proteomic databases

PRIDEiO32464.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12871; BAB12871; BAB12871.
GeneIDi1109597.
KEGGibuc:BU153.
PATRICi21243822. VBIBucAph127364_0163.

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228136.
KOiK04043.
OMAiFYSAKRF.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-153-MONOMER.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK. 1 hit.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAK_BUCAI
AccessioniPrimary (citable) accession number: O32464
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.