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O32463 (GSHB_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Synonyms:gshII
Ordered Locus Names:Synpcc7942_2324
OrganismSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]
Taxonomic identifier1140 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutathione synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197488

Regions

Domain133 – 317185ATP-grasp
Nucleotide binding159 – 21557ATP By similarity

Sites

Metal binding2881Magnesium or manganese By similarity
Metal binding2901Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
O32463 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 6BFE8564548FBF72

FASTA32335,418
        10         20         30         40         50         60 
MKLAFLIDPI AALDPTHDST VALMEAAQLR GHEIWIGEIS DLSVHVGEPL GLLRPLKIEP 

        70         80         90        100        110        120 
VQWLGDRWQV ANPWFTAGEA KRRSLHDFAA VFMRKDPPVT TAYLYATYLL DLVDPKKTRV 

       130        140        150        160        170        180 
VNSPEGLRHA NEKMYALQFQ SVVPRTLVSS NKAEIRAFLD ELRAAVLKPL GGKAGEGILF 

       190        200        210        220        230        240 
LDPGDRNFNS LVEISTQQGQ LPVMVQQYLP EAKDGDKRII LVNGEPLGAV NRVPTGREFR 

       250        260        270        280        290        300 
GNMAVGGRVE AVPITDRDRE ICAAVAPRLR QDGLFFVGID VIGGYLTEVN VTSPTGIREI 

       310        320 
DRLNGVSIGD QTIAALEALV NQG 

« Hide

References

« Hide 'large scale' references
[1]"The gshB gene in the cyanobacterium Synechococcus sp. PCC 7942 encodes a functional glutathione synthetase."
Okumura N., Masamoto K., Wada H.
Microbiology 143:2883-2890(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7942.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D88540 Genomic DNA. Translation: BAA22859.1.
CP000100 Genomic DNA. Translation: ABB58354.1.
RefSeqYP_401341.1. NC_007604.1.

3D structure databases

ProteinModelPortalO32463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1140.Synpcc7942_2324.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB58354; ABB58354; Synpcc7942_2324.
GeneID3774607.
KEGGsyf:Synpcc7942_2324.
PATRIC23790195. VBISynElo51371_2613.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

BioCycSYNEL:SYNPCC7942_2324-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_SYNE7
AccessionPrimary (citable) accession number: O32463
Secondary accession number(s): Q31KR5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways