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O32463

- GSHB_SYNE7

UniProt

O32463 - GSHB_SYNE7

Protein

Glutathione synthetase

Gene

gshB

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi288 – 2881Magnesium or manganeseUniRule annotation
    Metal bindingi290 – 2901Magnesium or manganeseUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi159 – 21557ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutathione synthase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSYNEL:SYNPCC7942_2324-MONOMER.
    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshBUniRule annotation
    Synonyms:gshII
    Ordered Locus Names:Synpcc7942_2324
    OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
    Taxonomic identifieri1140 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000002717: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323Glutathione synthetasePRO_0000197488Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi1140.Synpcc7942_2324.

    Structurei

    3D structure databases

    ProteinModelPortaliO32463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini133 – 317185ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic GSH synthase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0189.
    HOGENOMiHOG000265022.
    KOiK01920.
    OrthoDBiEOG6WMHWB.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_00162. GSH_S.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01380. glut_syn. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O32463-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLAFLIDPI AALDPTHDST VALMEAAQLR GHEIWIGEIS DLSVHVGEPL    50
    GLLRPLKIEP VQWLGDRWQV ANPWFTAGEA KRRSLHDFAA VFMRKDPPVT 100
    TAYLYATYLL DLVDPKKTRV VNSPEGLRHA NEKMYALQFQ SVVPRTLVSS 150
    NKAEIRAFLD ELRAAVLKPL GGKAGEGILF LDPGDRNFNS LVEISTQQGQ 200
    LPVMVQQYLP EAKDGDKRII LVNGEPLGAV NRVPTGREFR GNMAVGGRVE 250
    AVPITDRDRE ICAAVAPRLR QDGLFFVGID VIGGYLTEVN VTSPTGIREI 300
    DRLNGVSIGD QTIAALEALV NQG 323
    Length:323
    Mass (Da):35,418
    Last modified:January 1, 1998 - v1
    Checksum:i6BFE8564548FBF72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D88540 Genomic DNA. Translation: BAA22859.1.
    CP000100 Genomic DNA. Translation: ABB58354.1.
    RefSeqiYP_401341.1. NC_007604.1.

    Genome annotation databases

    EnsemblBacteriaiABB58354; ABB58354; Synpcc7942_2324.
    GeneIDi3774607.
    KEGGisyf:Synpcc7942_2324.
    PATRICi23790195. VBISynElo51371_2613.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D88540 Genomic DNA. Translation: BAA22859.1 .
    CP000100 Genomic DNA. Translation: ABB58354.1 .
    RefSeqi YP_401341.1. NC_007604.1.

    3D structure databases

    ProteinModelPortali O32463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 1140.Synpcc7942_2324.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB58354 ; ABB58354 ; Synpcc7942_2324 .
    GeneIDi 3774607.
    KEGGi syf:Synpcc7942_2324.
    PATRICi 23790195. VBISynElo51371_2613.

    Phylogenomic databases

    eggNOGi COG0189.
    HOGENOMi HOG000265022.
    KOi K01920.
    OrthoDBi EOG6WMHWB.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .
    BioCyci SYNEL:SYNPCC7942_2324-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_00162. GSH_S.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01380. glut_syn. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gshB gene in the cyanobacterium Synechococcus sp. PCC 7942 encodes a functional glutathione synthetase."
      Okumura N., Masamoto K., Wada H.
      Microbiology 143:2883-2890(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7942.

    Entry informationi

    Entry nameiGSHB_SYNE7
    AccessioniPrimary (citable) accession number: O32463
    Secondary accession number(s): Q31KR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3