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O32450 (MALQ_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-alpha-glucanotransferase

EC=2.4.1.25
Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme
Gene names
Ordered Locus Names:TK1809
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

Sequence similarities

Belongs to the glycosyl hydrolase 57 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6536534-alpha-glucanotransferase
PRO_0000184576

Sites

Active site1231Nucleophile By similarity
Active site2141Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
O32450 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: B0C4695613F29219

FASTA65376,665
        10         20         30         40         50         60 
MEMVNFIFGI HNHQPLGNFG WVMESAYERS YRPFMETLEE YPNMKVAVHY SGPLLEWIRD 

        70         80         90        100        110        120 
NKPEHLDLLR SLVKRGQLEI VVAGFYEPVL ASIPKEDRIV QIEKLKEFAR NLGYEARGVW 

       130        140        150        160        170        180 
LTERVWQPEL VKSLRAAGID YVIVDDYHFM SAGLSKDELF WPYYTEDGGE VITVFPIDEK 

       190        200        210        220        230        240 
LRYLIPFRPV DKTLEYLHSL DDGDESKVAV FHDDGEKFGV WPGTYEWVYE KGWLREFFDR 

       250        260        270        280        290        300 
VSSDERINLM LYSEYLQRFR PRGLVYLPIA SYFEMSEWSL PARQAKLFVE FVEELKKENK 

       310        320        330        340        350        360 
FDRYRVFVRG GIWKNFFFKY PESNYMHKRM LMVSKAVRNN PEAREFILRA QCNDAYWHGV 

       370        380        390        400        410        420 
FGGVYLPHLR RAVWENIIKA QSHVKTGNFV RDIDFDGRDE VFIENENFYA VFKPAYGGAL 

       430        440        450        460        470        480 
FELSSKRKAV NYNDVLARRW EHYHEVPEAA TPEEGGEGVA SIHELGKQIP DEIRRELAYD 

       490        500        510        520        530        540 
SHLRAILQDH FLEPETTLDE YRLSRYIELG DFLTGAYNFS LIENGITLER DGSVAKRPAR 

       550        560        570        580        590        600 
VEKSVRLTED GFIVDYTVRS DARALFGVEL NLAVHSVMEE PAEFEAEKFE VNDPYGIGKV 

       610        620        630        640        650 
EIELDRRAKV WKYPIKTLSQ SESGWDFIQQ GVSYTVLFPV EGELRFRLRF REL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of the 4-alpha-glucanotransferase gene from the hyperthermophilic archaeon Pyrococcus sp. KOD1, and characterization of the enzyme."
Tachibana Y., Fujiwara S., Takagi M., Imanaka T.
J. Ferment. Bioeng. 83:540-548(1997)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87907 Genomic DNA. Translation: BAA22062.1.
AP006878 Genomic DNA. Translation: BAD85998.1.
RefSeqYP_184222.1. NC_006624.1.

3D structure databases

ProteinModelPortalO32450.
SMRO32450. Positions 2-653.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK1809.

Protein family/group databases

CAZyGH57. Glycoside Hydrolase Family 57.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD85998; BAD85998; TK1809.
GeneID3235018.
KEGGtko:TK1809.

Phylogenomic databases

eggNOGCOG1449.
HOGENOMHOG000223207.
KOK01176.
OMAHFMSAGL.
ProtClustDBCLSK2459743.

Enzyme and pathway databases

BioCycTKOD69014:GH72-1846-MONOMER.

Family and domain databases

Gene3D2.70.98.10. 1 hit.
3.20.110.10. 2 hits.
InterProIPR015179. A-amylase/a-glucTrfase_C.
IPR015178. A-amylase/a-glucTrfase_central.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR004300. Glyco_hydro_57_N.
[Graphical view]
PfamPF09094. DUF1925. 1 hit.
PF09095. DUF1926. 1 hit.
PF03065. Glyco_hydro_57. 1 hit.
[Graphical view]
ProDomPD014405. DUF1925. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF74650. SSF74650. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMALQ_THEKO
AccessionPrimary (citable) accession number: O32450
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries