O32450 (MALQ_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-alpha-glucanotransferase
EC=2.4.1.25

Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme

Gene names

Ordered Locus Names:

TK1809

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus kodakarensis

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Sequence similarities	Belongs to the glycosyl hydrolase 57 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	4-alpha-glucanotransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 653	653	4-alpha-glucanotransferase		PRO_0000184576
Sites
	Active site	123	1	Nucleophile By similarity
	Active site	214	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O32450 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B0C4695613F29219	FASTA	653	76,665
	10 20 30 40 50 60 MEMVNFIFGI HNHQPLGNFG WVMESAYERS YRPFMETLEE YPNMKVAVHY SGPLLEWIRD 70 80 90 100 110 120 NKPEHLDLLR SLVKRGQLEI VVAGFYEPVL ASIPKEDRIV QIEKLKEFAR NLGYEARGVW 130 140 150 160 170 180 LTERVWQPEL VKSLRAAGID YVIVDDYHFM SAGLSKDELF WPYYTEDGGE VITVFPIDEK 190 200 210 220 230 240 LRYLIPFRPV DKTLEYLHSL DDGDESKVAV FHDDGEKFGV WPGTYEWVYE KGWLREFFDR 250 260 270 280 290 300 VSSDERINLM LYSEYLQRFR PRGLVYLPIA SYFEMSEWSL PARQAKLFVE FVEELKKENK 310 320 330 340 350 360 FDRYRVFVRG GIWKNFFFKY PESNYMHKRM LMVSKAVRNN PEAREFILRA QCNDAYWHGV 370 380 390 400 410 420 FGGVYLPHLR RAVWENIIKA QSHVKTGNFV RDIDFDGRDE VFIENENFYA VFKPAYGGAL 430 440 450 460 470 480 FELSSKRKAV NYNDVLARRW EHYHEVPEAA TPEEGGEGVA SIHELGKQIP DEIRRELAYD 490 500 510 520 530 540 SHLRAILQDH FLEPETTLDE YRLSRYIELG DFLTGAYNFS LIENGITLER DGSVAKRPAR 550 560 570 580 590 600 VEKSVRLTED GFIVDYTVRS DARALFGVEL NLAVHSVMEE PAEFEAEKFE VNDPYGIGKV 610 620 630 640 650 EIELDRRAKV WKYPIKTLSQ SESGWDFIQQ GVSYTVLFPV EGELRFRLRF REL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the 4-alpha-glucanotransferase gene from the hyperthermophilic archaeon Pyrococcus sp. KOD1, and characterization of the enzyme." Tachibana Y., Fujiwara S., Takagi M., Imanaka T. J. Ferment. Bioeng. 83:540-548(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87907 Genomic DNA. Translation: BAA22062.1. AP006878 Genomic DNA. Translation: BAD85998.1.
RefSeq	YP_184222.1. NC_006624.1.
3D structure databases
ProteinModelPortal	O32450.
SMR	O32450. Positions 2-653.
ModBase	Search...
Protein-protein interaction databases
STRING	69014.TK1809.
Protein family/group databases
CAZy	GH57. Glycoside Hydrolase Family 57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD85998; BAD85998; TK1809.
GeneID	3235018.
KEGG	tko:TK1809.
Phylogenomic databases
eggNOG	COG1449.
HOGENOM	HOG000223207.
KO	K01176.
OMA	HFMSAGL.
ProtClustDB	CLSK2459743.
Enzyme and pathway databases
BioCyc	TKOD69014:GH72-1846-MONOMER.
Family and domain databases
Gene3D	2.70.98.10. 1 hit. 3.20.110.10. 2 hits.
InterPro	IPR015179. A-amylase/a-glucTrfase_C. IPR015178. A-amylase/a-glucTrfase_central. IPR011013. Gal_mutarotase_SF_dom. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR027291. Glyco_hydro_38/57_N. IPR004300. Glyco_hydro_57_N. [Graphical view]
Pfam	PF09094. DUF1925. 1 hit. PF09095. DUF1926. 1 hit. PF03065. Glyco_hydro_57. 1 hit. [Graphical view]
ProDom	PD014405. DUF1925. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MALQ_PYRKO

Accession

Primary (citable) accession number: O32450

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program