Reviewed, UniProtKB/Swiss-Prot O32445 (NAGA_VIBCH)

Names and origin

Protein names

Recommended name:
    N-acetylglucosamine-6-phosphate deacetylase
    EC=3.5.1.25
Alternative name(s):
    GlcNAc 6-P deacetylase

Gene names

Name:	nagA
Ordered Locus Names:	VC_0994

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate.
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 4/5.
Sequence similarities	Belongs to the nagA family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	N-acetylglucosamine-6-phosphate deacetylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 378

378

N-acetylglucosamine-6-phosphate deacetylase

PRO_0000170918

Experimental info

Sequence conflict

79

1

T → I in BAA22834. Ref.1

Secondary structure

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378

Helix Strand Turn

Details...

Beta strand

2 – 10

9

Beta strand

15 – 23

9

Beta strand

27 – 33

7

Helix

34 – 36

3

Beta strand

42 – 45

4

Beta strand

50 – 52

3

Beta strand

56 – 60

5

Turn

68 – 70

3

Helix

74 – 86

13

Beta strand

91 – 98

8

Helix

101 – 117

17

Beta strand

119 – 122

4

Beta strand

126 – 128

3

Helix

134 – 136

3

Turn

142 – 144

3

Helix

150 – 158

9

Turn

159 – 161

3

Beta strand

165 – 167

3

Helix

175 – 183

9

Beta strand

187 – 190

4

Helix

197 – 205

9

Beta strand

210 – 213

4

Beta strand

216 – 219

4

Helix

232 – 234

3

Helix

251 – 260

10

Beta strand

265 – 268

4

Beta strand

280 – 283

4

Beta strand

288 – 291

4

Helix

310 – 319

10

Helix

325 – 332

8

Helix

334 – 339

6

Turn

343 – 345

3

Beta strand

357 – 360

4

Beta strand

366 – 370

5

Beta strand

375 – 377

3

Sequences

	Sequence		Length	Mass (Da)
	O32445-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 34906344A3F92A0F	FASTA	378	40,956
	10 20 30 40 50 60 MYALTNCKIY TGNDVLVKHA VIINGDKIEA VCPIESLPSE MNVVDLNGAN LSPGFIDLQL 70 80 90 100 110 120 NGCGGVMFND EITAETIDTM HKANLKSGCT SFLPTLITSS DENMRQAIAA AREYQAKYPN 130 140 150 160 170 180 QSLGLHLEGP YLNVMKKGIH SVDFIRPSDD TMIDTICANS DVIAKVTLAP ENNKPEHIEK 190 200 210 220 230 240 LVKAGIVVSI GHTNATYSEA RKSFESGITF ATHLFNAMTP MVGREPGVVG AIYDTPEVYA 250 260 270 280 290 300 GIIADGFHVD YANIRIAHKI KGEKLVLVTD ATAPAGAEMD YFIFVGKKVY YRDGKCVDEN 310 320 330 340 350 360 GTLGGSALTM IEAVQNTVEH VGIALDEALR MATLYPAKAI GVDEKLGRIK KGMIANLTVF 370 DRDFNVKATV VNGQYEQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the genes for N-acetylglucosamine use that construct divergent operons (nagE-nagAC) from Vibrio cholerae non-O1." Yamano N., Oura N., Wang J., Fujishima S. Biosci. Biotechnol. Biochem. 61:1349-1353(1997) [PubMed: 9301118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NON-O1 / 1148A.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D87820 Genomic DNA. Translation: BAA22834.1.
AE003852 Genomic DNA. Translation: AAF94155.1.

PIR

E82254.
JC5649.

RefSeq

NP_230640.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EGJ	X-ray	2.90	A/B	1-378	[»]
3IV8	X-ray	2.53	A/B/C/D	1-378	[»]

ModBase

Search...

Genome annotation databases

GeneID

2614247.

GenomeReviews

Gene locus VC_0994 in contig AE003852_GR.

KEGG

vch:VC0994.

TIGR

VC_0994.

Phylogenomic databases

HOGENOM

HBG464950.

OMA

TSSDEDM.

Enzyme and pathway databases

BRENDA

3.5.1.25. 19019.

Family and domain databases

InterPro

IPR006680. Amidohydro_1.
IPR003764. GlcNAc_6-P_deAcase.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]

Pfam

PF01979. Amidohydro_1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00221. nagA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NAGA_VIBCH

Accession

Primary (citable) accession number: O32445
Secondary accession number(s): Q9KTA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)