ID   PPOX_PROFF              Reviewed;         527 AA.
AC   O32434;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Protoporphyrinogen oxidase;
DE            Short=PPO;
DE            EC=1.3.3.4;
GN   Name=hemY;
OS   Propionibacterium freudenreichii subsp. freudenreichii.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Propionibacterineae; Propionibacteriaceae; Propionibacterium.
OX   NCBI_TaxID=66712;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 6207 / DSM 20271 / IFO 12424 / LMG 16412 / NCTC 10470;
RX   MEDLINE=97306686; PubMed=9163953; DOI=10.1007/s002530050945;
RA   Hashimoto Y., Yamashita Y., Murooka Y.;
RT   "The Propionibacterium freudenreichii hemYHBXRL gene cluster, which
RT   encodes enzymes and a regulator involved in the biosynthetic pathway
RT   from glutamate to protoheme.";
RL   Appl. Microbiol. Biotechnol. 47:385-392(1997).
CC   -!- FUNCTION: Catalyzes the 6-electron oxidation of
CC       protoporphyrinogen-IX to form protoporphyrin-IX.
CC   -!- CATALYTIC ACTIVITY: Protoporphyrinogen-IX + 3 O(2) =
CC       protoporphyrin-IX + 3 H(2)O(2).
CC   -!- COFACTOR: Binds 1 FAD per dimer (By similarity).
CC   -!- PATHWAY: Porphyrin metabolism; protoporphyrin-IX biosynthesis;
CC       protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the protoporphyrinogen oxidase family.
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DR   EMBL; D85417; BAA21909.1; -; Genomic_DNA.
DR   BRENDA; 1.3.3.4; 291591.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0004729; F:protoporphyrinogen oxidase activity; IEA:EC.
DR   GO; GO:0006783; P:heme biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR004572; Protoporphyrinogen_oxidase.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   TIGRFAMs; TIGR00562; proto_IX_ox; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; Heme biosynthesis; Oxidoreductase;
KW   Porphyrin biosynthesis.
FT   CHAIN         1    527       Protoporphyrinogen oxidase.
FT                                /FTId=PRO_0000135268.
FT   NP_BIND      33     38       FAD (Potential).
SQ   SEQUENCE   527 AA;  55268 MW;  579CB0A3318BF938 CRC64;
     MSTTDRVTTP TPTVSGTDAP GPDASHCHLV VVGGGITGLA AAWQGMARGA RVSVVESDDH
     FGGKVVTDRR DGFLVEQGPD SFVAYRPAAL KLIEELGLSD QVIAPGGGRR VSLLSRGKLR
     PMPAGMGMVL PTRMWPFVTT TVLSWPDKIR AGLDLVIPRR LPDHDVAIGA FLRQRLGDGI
     VRRFADPMVG GIYGAGIDEL SLDAVLPSLR DNERDHRSLM VASLAGGRAS RRAARQRAAQ
     NNAQQNSSHQ NSTGQNNSAG TRGPAASPFR TLRGGLGQLI DALVDQLRAG GVELLVNTSV
     DLLGRDGVHL SDGRVLPADA VVLAGGVASS ARLLRPQLPA AARALAQIPL ASTTIVSLAW
     PVSAFDVAPD SQGWLEADAG PVSGLTASSI KFAGRAPDGS VLMRVFVPDK RGPLTDAPDD
     ELLSAVIDHV RPLLGVHGEP GLTQITRWHK VMPKYTVGHL ERAAVVDSTL AEQRPTWAVA
     GSALHGVGLP DCISDARHSA DEVIDAALAA TPSAPNRNAA TDRTETR
//