Reviewed, UniProtKB/Swiss-Prot O32434 (PPOX_PROFF)

Names and origin

Protein names

Recommended name:
    Protoporphyrinogen oxidase
      Short name=PPO
    EC=1.3.3.4

Gene names

Name:

hemY

Organism

Propionibacterium freudenreichii subsp. freudenreichii

Taxonomic identifier

66712 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Propionibacteriaceae › Propionibacterium

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	Binds 1 FAD per dimer By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro protoporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Protoporphyrinogen oxidase		PRO_0000135268
Regions
	Nucleotide binding	33 – 38	6	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	O32434-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 579CB0A3318BF938	FASTA	527	55,268
	10 20 30 40 50 60 MSTTDRVTTP TPTVSGTDAP GPDASHCHLV VVGGGITGLA AAWQGMARGA RVSVVESDDH 70 80 90 100 110 120 FGGKVVTDRR DGFLVEQGPD SFVAYRPAAL KLIEELGLSD QVIAPGGGRR VSLLSRGKLR 130 140 150 160 170 180 PMPAGMGMVL PTRMWPFVTT TVLSWPDKIR AGLDLVIPRR LPDHDVAIGA FLRQRLGDGI 190 200 210 220 230 240 VRRFADPMVG GIYGAGIDEL SLDAVLPSLR DNERDHRSLM VASLAGGRAS RRAARQRAAQ 250 260 270 280 290 300 NNAQQNSSHQ NSTGQNNSAG TRGPAASPFR TLRGGLGQLI DALVDQLRAG GVELLVNTSV 310 320 330 340 350 360 DLLGRDGVHL SDGRVLPADA VVLAGGVASS ARLLRPQLPA AARALAQIPL ASTTIVSLAW 370 380 390 400 410 420 PVSAFDVAPD SQGWLEADAG PVSGLTASSI KFAGRAPDGS VLMRVFVPDK RGPLTDAPDD 430 440 450 460 470 480 ELLSAVIDHV RPLLGVHGEP GLTQITRWHK VMPKYTVGHL ERAAVVDSTL AEQRPTWAVA 490 500 510 520 GSALHGVGLP DCISDARHSA DEVIDAALAA TPSAPNRNAA TDRTETR

References

[1]

"The Propionibacterium freudenreichii hemYHBXRL gene cluster, which encodes enzymes and a regulator involved in the biosynthetic pathway from glutamate to protoheme." Hashimoto Y., Yamashita Y., Murooka Y. Appl. Microbiol. Biotechnol. 47:385-392(1997) [PubMed: 9163953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6207 / DSM 20271 / IFO 12424 / LMG 16412 / NCTC 10470.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D85417 Genomic DNA. Translation: BAA21909.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.3.4. 291591.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. IPR016040. NAD(P)-bd_dom. IPR004572. Protoporphyrinogen_oxidase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00562. proto_IX_ox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPOX_PROFF

Accession

Primary (citable) accession number: O32434

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)