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Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Propionibacterium freudenreichii subsp. freudenreichii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathway: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase (hemY)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641FAD; via amide nitrogenBy similarity
Binding sitei300 – 3001FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei448 – 4481FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 386FADBy similarity
Nucleotide bindingi56 – 572FADBy similarity
Nucleotide bindingi78 – 814FADBy similarity
Nucleotide bindingi487 – 4893FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:hemY
OrganismiPropionibacterium freudenreichii subsp. freudenreichii
Taxonomic identifieri66712 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesPropionibacteriaceaePropionibacterium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Protoporphyrinogen oxidasePRO_0000135268Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO32434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

O32434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTDRVTTP TPTVSGTDAP GPDASHCHLV VVGGGITGLA AAWQGMARGA
60 70 80 90 100
RVSVVESDDH FGGKVVTDRR DGFLVEQGPD SFVAYRPAAL KLIEELGLSD
110 120 130 140 150
QVIAPGGGRR VSLLSRGKLR PMPAGMGMVL PTRMWPFVTT TVLSWPDKIR
160 170 180 190 200
AGLDLVIPRR LPDHDVAIGA FLRQRLGDGI VRRFADPMVG GIYGAGIDEL
210 220 230 240 250
SLDAVLPSLR DNERDHRSLM VASLAGGRAS RRAARQRAAQ NNAQQNSSHQ
260 270 280 290 300
NSTGQNNSAG TRGPAASPFR TLRGGLGQLI DALVDQLRAG GVELLVNTSV
310 320 330 340 350
DLLGRDGVHL SDGRVLPADA VVLAGGVASS ARLLRPQLPA AARALAQIPL
360 370 380 390 400
ASTTIVSLAW PVSAFDVAPD SQGWLEADAG PVSGLTASSI KFAGRAPDGS
410 420 430 440 450
VLMRVFVPDK RGPLTDAPDD ELLSAVIDHV RPLLGVHGEP GLTQITRWHK
460 470 480 490 500
VMPKYTVGHL ERAAVVDSTL AEQRPTWAVA GSALHGVGLP DCISDARHSA
510 520
DEVIDAALAA TPSAPNRNAA TDRTETR
Length:527
Mass (Da):55,268
Last modified:January 1, 1998 - v1
Checksum:i579CB0A3318BF938
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85417 Genomic DNA. Translation: BAA21909.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85417 Genomic DNA. Translation: BAA21909.1.

3D structure databases

ProteinModelPortaliO32434.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Propionibacterium freudenreichii hemYHBXRL gene cluster, which encodes enzymes and a regulator involved in the biosynthetic pathway from glutamate to protoheme."
    Hashimoto Y., Yamashita Y., Murooka Y.
    Appl. Microbiol. Biotechnol. 47:385-392(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 6207 / DSM 20271 / LMG 16412 / NBRC 12424 / NCIMB 5959 / NCTC 10470 / NRRL B-3523.

Entry informationi

Entry nameiPPOX_PROFF
AccessioniPrimary (citable) accession number: O32434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.