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O32434 (PPOX_PROFF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protoporphyrinogen oxidase

Short name=PPO
EC=1.3.3.4
Gene names
Name:hemY
OrganismPropionibacterium freudenreichii subsp. freudenreichii
Taxonomic identifier66712 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

Catalytic activity

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactor

Binds 1 FAD per subunit By similarity.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionoxygen-dependent protoporphyrinogen oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Protoporphyrinogen oxidase
PRO_0000135268

Regions

Nucleotide binding33 – 386FAD By similarity
Nucleotide binding56 – 572FAD By similarity
Nucleotide binding78 – 814FAD By similarity
Nucleotide binding487 – 4893FAD By similarity

Sites

Binding site641FAD; via amide nitrogen By similarity
Binding site3001FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding site4481FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
O32434 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 579CB0A3318BF938

FASTA52755,268
        10         20         30         40         50         60 
MSTTDRVTTP TPTVSGTDAP GPDASHCHLV VVGGGITGLA AAWQGMARGA RVSVVESDDH 

        70         80         90        100        110        120 
FGGKVVTDRR DGFLVEQGPD SFVAYRPAAL KLIEELGLSD QVIAPGGGRR VSLLSRGKLR 

       130        140        150        160        170        180 
PMPAGMGMVL PTRMWPFVTT TVLSWPDKIR AGLDLVIPRR LPDHDVAIGA FLRQRLGDGI 

       190        200        210        220        230        240 
VRRFADPMVG GIYGAGIDEL SLDAVLPSLR DNERDHRSLM VASLAGGRAS RRAARQRAAQ 

       250        260        270        280        290        300 
NNAQQNSSHQ NSTGQNNSAG TRGPAASPFR TLRGGLGQLI DALVDQLRAG GVELLVNTSV 

       310        320        330        340        350        360 
DLLGRDGVHL SDGRVLPADA VVLAGGVASS ARLLRPQLPA AARALAQIPL ASTTIVSLAW 

       370        380        390        400        410        420 
PVSAFDVAPD SQGWLEADAG PVSGLTASSI KFAGRAPDGS VLMRVFVPDK RGPLTDAPDD 

       430        440        450        460        470        480 
ELLSAVIDHV RPLLGVHGEP GLTQITRWHK VMPKYTVGHL ERAAVVDSTL AEQRPTWAVA 

       490        500        510        520 
GSALHGVGLP DCISDARHSA DEVIDAALAA TPSAPNRNAA TDRTETR 

« Hide

References

[1]"The Propionibacterium freudenreichii hemYHBXRL gene cluster, which encodes enzymes and a regulator involved in the biosynthetic pathway from glutamate to protoheme."
Hashimoto Y., Yamashita Y., Murooka Y.
Appl. Microbiol. Biotechnol. 47:385-392(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6207 / DSM 20271 / LMG 16412 / NBRC 12424 / NCIMB 5959 / NCTC 10470 / NRRL B-3523.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D85417 Genomic DNA. Translation: BAA21909.1.

3D structure databases

ProteinModelPortalO32434.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00251; UER00324.

Family and domain databases

Gene3D3.90.660.20. 1 hit.
InterProIPR002937. Amino_oxidase.
IPR004572. Protoporphyrinogen_oxidase.
IPR027418. Protoporphyrinogen_oxidase_C.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00562. proto_IX_ox. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPOX_PROFF
AccessionPrimary (citable) accession number: O32434
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways