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O32434

- PPOX_PROFF

UniProt

O32434 - PPOX_PROFF

Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Propionibacterium freudenreichii subsp. freudenreichii
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

    Catalytic activityi

    Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641FAD; via amide nitrogenBy similarity
    Binding sitei300 – 3001FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei448 – 4481FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 386FADBy similarity
    Nucleotide bindingi56 – 572FADBy similarity
    Nucleotide bindingi78 – 814FADBy similarity
    Nucleotide bindingi487 – 4893FADBy similarity

    GO - Molecular functioni

    1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00324.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protoporphyrinogen oxidase (EC:1.3.3.4)
    Short name:
    PPO
    Gene namesi
    Name:hemY
    OrganismiPropionibacterium freudenreichii subsp. freudenreichii
    Taxonomic identifieri66712 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 527527Protoporphyrinogen oxidasePRO_0000135268Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO32434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the protoporphyrinogen oxidase family.Curated

    Family and domain databases

    Gene3Di3.90.660.20. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O32434-1 [UniParc]FASTAAdd to Basket

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    MSTTDRVTTP TPTVSGTDAP GPDASHCHLV VVGGGITGLA AAWQGMARGA    50
    RVSVVESDDH FGGKVVTDRR DGFLVEQGPD SFVAYRPAAL KLIEELGLSD 100
    QVIAPGGGRR VSLLSRGKLR PMPAGMGMVL PTRMWPFVTT TVLSWPDKIR 150
    AGLDLVIPRR LPDHDVAIGA FLRQRLGDGI VRRFADPMVG GIYGAGIDEL 200
    SLDAVLPSLR DNERDHRSLM VASLAGGRAS RRAARQRAAQ NNAQQNSSHQ 250
    NSTGQNNSAG TRGPAASPFR TLRGGLGQLI DALVDQLRAG GVELLVNTSV 300
    DLLGRDGVHL SDGRVLPADA VVLAGGVASS ARLLRPQLPA AARALAQIPL 350
    ASTTIVSLAW PVSAFDVAPD SQGWLEADAG PVSGLTASSI KFAGRAPDGS 400
    VLMRVFVPDK RGPLTDAPDD ELLSAVIDHV RPLLGVHGEP GLTQITRWHK 450
    VMPKYTVGHL ERAAVVDSTL AEQRPTWAVA GSALHGVGLP DCISDARHSA 500
    DEVIDAALAA TPSAPNRNAA TDRTETR 527
    Length:527
    Mass (Da):55,268
    Last modified:January 1, 1998 - v1
    Checksum:i579CB0A3318BF938
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D85417 Genomic DNA. Translation: BAA21909.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D85417 Genomic DNA. Translation: BAA21909.1 .

    3D structure databases

    ProteinModelPortali O32434.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00324 .

    Family and domain databases

    Gene3Di 3.90.660.20. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Propionibacterium freudenreichii hemYHBXRL gene cluster, which encodes enzymes and a regulator involved in the biosynthetic pathway from glutamate to protoheme."
      Hashimoto Y., Yamashita Y., Murooka Y.
      Appl. Microbiol. Biotechnol. 47:385-392(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 6207 / DSM 20271 / LMG 16412 / NBRC 12424 / NCIMB 5959 / NCTC 10470 / NRRL B-3523.

    Entry informationi

    Entry nameiPPOX_PROFF
    AccessioniPrimary (citable) accession number: O32434
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3