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Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Propionibacterium freudenreichii subsp. freudenreichii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase (hemY)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64FAD; via amide nitrogenBy similarity1
Binding sitei300FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei448FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 38FADBy similarity6
Nucleotide bindingi56 – 57FADBy similarity2
Nucleotide bindingi78 – 81FADBy similarity4
Nucleotide bindingi487 – 489FADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:hemY
OrganismiPropionibacterium freudenreichii subsp. freudenreichii
Taxonomic identifieri66712 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesPropionibacteriaceaePropionibacterium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352681 – 527Protoporphyrinogen oxidaseAdd BLAST527

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO32434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 3 hits.
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

O32434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTDRVTTP TPTVSGTDAP GPDASHCHLV VVGGGITGLA AAWQGMARGA
60 70 80 90 100
RVSVVESDDH FGGKVVTDRR DGFLVEQGPD SFVAYRPAAL KLIEELGLSD
110 120 130 140 150
QVIAPGGGRR VSLLSRGKLR PMPAGMGMVL PTRMWPFVTT TVLSWPDKIR
160 170 180 190 200
AGLDLVIPRR LPDHDVAIGA FLRQRLGDGI VRRFADPMVG GIYGAGIDEL
210 220 230 240 250
SLDAVLPSLR DNERDHRSLM VASLAGGRAS RRAARQRAAQ NNAQQNSSHQ
260 270 280 290 300
NSTGQNNSAG TRGPAASPFR TLRGGLGQLI DALVDQLRAG GVELLVNTSV
310 320 330 340 350
DLLGRDGVHL SDGRVLPADA VVLAGGVASS ARLLRPQLPA AARALAQIPL
360 370 380 390 400
ASTTIVSLAW PVSAFDVAPD SQGWLEADAG PVSGLTASSI KFAGRAPDGS
410 420 430 440 450
VLMRVFVPDK RGPLTDAPDD ELLSAVIDHV RPLLGVHGEP GLTQITRWHK
460 470 480 490 500
VMPKYTVGHL ERAAVVDSTL AEQRPTWAVA GSALHGVGLP DCISDARHSA
510 520
DEVIDAALAA TPSAPNRNAA TDRTETR
Length:527
Mass (Da):55,268
Last modified:January 1, 1998 - v1
Checksum:i579CB0A3318BF938
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85417 Genomic DNA. Translation: BAA21909.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85417 Genomic DNA. Translation: BAA21909.1.

3D structure databases

ProteinModelPortaliO32434.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 3 hits.
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPOX_PROFF
AccessioniPrimary (citable) accession number: O32434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.