Reviewed, UniProtKB/Swiss-Prot O32333 (PTHB_CLOB8)

Names and origin

Protein names

Recommended name:
    Glucitol/sorbitol-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
Alternative name(s):
    PTS system glucitol/sorbitol-specific EIIB component
    EII-Gut

Gene names

Name:	srlE
Synonyms:	gutA2
Ordered Locus Names:	Cbei_0337

Organism

Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]

Taxonomic identifier

290402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucitol/sorbitol transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-5 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Glucitol/sorbitol-specific phosphotransferase enzyme IIB component		PRO_0000186564
Regions
	Transmembrane	194 – 214	21	Potential
	Transmembrane	228 – 248	21	Potential
	Transmembrane	250 – 270	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	312 – 332	21	Potential
	Domain	3 – 195	193	PTS EIIB type-5
	Region	196 – 336	141	Unknown
Sites
	Active site	75	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O32333-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D766D0501EA10694	FASTA	336	35,174
	10 20 30 40 50 60 MEKYNAIKIV KGSGGFGGPL TVKPEEGKDT LLYITGGGAE PEIVEKIVNL TGCKAVNGFK 70 80 90 100 110 120 TSVPEEQIFL VIIDCGGTLR CGIYPQKRIP TINVMPVGKS GPLAKFITED IYVSAVGLNQ 130 140 150 160 170 180 ISLADSSAEP IKSTKVPEEG KREFKYSADK KVSQSLAENS KSSIVQKIGM GAGKVVNTLY 190 200 210 220 230 240 QAGRDAVQSM ITTILPFMAF VAMLIGIIQG SGFGNWFAKI LVPLAGNGIG LMILGFICSI 250 260 270 280 290 300 PLLSALLGPG AVIAQIVGTL IGVEIGKGTI PPSLALPALF AINTQCACDF IPVGLGLAEA 310 320 330 EPETVEVGVP SVLYSRFMIG VPRVAVAWVA SIGLYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene system for glucitol transport and metabolism in Clostridium beijerinckii NCIMB 8052." Tangney M., Brehm J.K., Minton N.P., Mitchell W.J. Appl. Environ. Microbiol. 64:1612-1619(1998) [PubMed: 9572925] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Clostridium beijerinckii NCIMB 8052." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G., Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W., Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B., Blaschek H., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ002527 Genomic DNA. Translation: CAA05514.1. CP000721 Genomic DNA. Translation: ABR32525.1.
RefSeq	YP_001307481.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O32333.
Genome annotation databases
GeneID	5291569.
GenomeReviews	Gene locus Cbei_0337 in contig CP000721_GR.
KEGG	cbe:Cbei_0337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YPKKGIP.
Family and domain databases
InterPro	IPR011638. PTS_EIIBC_GUT_C. IPR011618. PTS_EIIBC_GUT_N. IPR004702. PTS_sorb_EIIBC. [Graphical view]
Pfam	PF07663. EIIBC-GUT_C. 1 hit. PF03612. EIIBC-GUT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00825. EIIBC-GUT. 1 hit.
PROSITE	PS51102. PTS_EIIB_TYPE_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTHB_CLOB8

Accession

Primary (citable) accession number: O32333
Secondary accession number(s): A6LQ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)