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O32328 (ATKB_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Potassium-transporting ATPase B chain
EC=3.6.3.12
Alternative name(s):
ATP phosphohydrolase [potassium-transporting] B chain
Potassium-binding and translocating subunit B
Potassium-translocating ATPase B chain
Gene names
Name:kdpB
Ordered Locus Names:CA_C3681
OrganismClostridium acetobutylicum
Taxonomic identifier1488 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length685 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions. HAMAP MF_00285

Catalytic activity

ATP + H2O + K+(Out) = ADP + phosphate + K+(In). HAMAP MF_00285

Subcellular location

Cell membrane; Multi-pass membrane protein HAMAP MF_00285.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IA subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 685685Potassium-transporting ATPase B chain HAMAP MF_00285
PRO_0000046113

Regions

Topological domain1 – 3232Cytoplasmic Potential
Transmembrane33 – 5523Helical; Potential
Topological domain56 – 649Extracellular Potential
Transmembrane65 – 8420Helical; Potential
Topological domain85 – 217133Cytoplasmic Potential
Transmembrane218 – 24023Helical; Potential
Topological domain241 – 25414Extracellular Potential
Transmembrane255 – 27723Helical; Potential
Topological domain278 – 573296Cytoplasmic Potential
Transmembrane574 – 59623Helical; Potential
Topological domain597 – 61014Extracellular Potential
Transmembrane611 – 63323Helical; Potential
Topological domain634 – 65320Cytoplasmic Potential
Transmembrane654 – 67623Helical; Potential
Topological domain677 – 6859Extracellular Potential

Sites

Active site30614-aspartylphosphate intermediate Probable
Metal binding5171Magnesium By similarity
Metal binding5211Magnesium By similarity

Experimental info

Sequence conflict25 – 262LN → IK in AAC45478. Ref.1
Sequence conflict2561A → R in AAC45478. Ref.1
Sequence conflict2681G → A in AAC45478. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O32328 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: FD57B24E938F4402

FASTA68573,686
        10         20         30         40         50         60 
MKSKKSKFIT KDILKEAIIE SFKKLNPKYM MKNPVMFVVE VGFFVTILLT IFPSIFGDKG 

        70         80         90        100        110        120 
HNLRVYNLIV TIILFITVLF ANFAESVAEG RGKAQADALK KTRKDTIAKL IGKDGSIKTI 

       130        140        150        160        170        180 
NANELKKGDV VLVENGDVIP NDGEVVDGVA SVDESAITGE SAPVMKEPGG DFASVTGGTK 

       190        200        210        220        230        240 
VVSDWLKVEI TATPGESFLD KMINLVEGAS RQKTPNEIAL NTILVSLTLI FLIVLVALYP 

       250        260        270        280        290        300 
MATYTGVKIP MSTLIALLVC LIPTTIGGLL SAIGIAGMDR VTRFNVIAMS GKAVEACGDV 

       310        320        330        340        350        360 
DTMILDKTGT ITYGNRLAAD FITVGGADKQ KLIDYSVMCS LKDDTPEGKS IVELGKQLGI 

       370        380        390        400        410        420 
TIDTKKYESI EFEEFTAQTR MSGIKLENGT AVKKGAYDAI KKRVQELKGV IPKDLDEAVN 

       430        440        450        460        470        480 
KVAKLGGTPL VVCVDNKIYG VIYLKDTVKP GLVERFERLR EIGIKTIMCT GDNPLTAATI 

       490        500        510        520        530        540 
AKEAGVDGFI AECKPEDKIE AIKKEQDEGK LVAMTGDGTN DAPALAQADV GLAMNSGTTA 

       550        560        570        580        590        600 
AKEAANMVDL DSDPTKVLEV VEIGKQLLIT RGALTTFSIA NDVAKYFAII PAIFTIAIPK 

       610        620        630        640        650        660 
MQLMNIMHLS TPYSAILSAL IFNAIIIPAL IPIAMKGVKY RPMKSEALLL RNMIVFGFGG 

       670        680 
IIVPFVGIKI IDMIITPMVR ILNLG

« Hide

References

« Hide 'large scale' references
[1]"The kdp system of Clostridium acetobutylicum: cloning, sequencing, and transcriptional regulation in response to potassium concentration."
Treuner-Lange A., Kuhn A., Duerre P.
J. Bacteriol. 179:4501-4512(1997) [PubMed: 9226259] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U44892 Genomic DNA. Translation: AAC45478.1.
AE001437 Genomic DNA. Translation: AAK81602.1.
PIRG97351.
T46843.
RefSeqNP_350262.1. NC_003030.1.

3D structure databases

ProteinModelPortalO32328.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1119863.
GenomeReviewsGene locus CA_C3681 in contig AE001437_GR.
KEGGcac:CA_C3681.
NMPDRfig|272562.1.peg.3791.
PATRIC32041829. VBICloAce74127_3870.

Phylogenomic databases

HOGENOMHBG289193.
OMAIGIKTIM.
PhylomeDBO32328.
ProtClustDBPRK01122.

Enzyme and pathway databases

BioCycCACE272562:CAC3681-MONOMER.

Family and domain databases

HAMAPMF_00285. KdpB.
[Tree]
InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR006391. K-transp_ATPase_bsu.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KOK01547.
PANTHERPTHR24093:SF27. PTHR24093:SF27. 1 hit.
PfamPF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01494. ATPase_P-type. 2 hits.
TIGR01497. KdpB. 1 hit.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameATKB_CLOAB
AccessionPrimary (citable) accession number: O32328
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 29, 2001
Last modified: January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents