ID   SDPI_BACSU              Reviewed;         207 AA.
AC   O32241;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 38.
DE   RecName: Full=Immunity protein sdpI;
GN   Name=sdpI; Synonyms=yvaZ; OrderedLocusNames=BSU33780;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   FUNCTION, INDUCTION, AND MUTAGENESIS OF ILE-6; ILE-50; PHE-78; MET-84;
RP   LEU-85; ILE-98; GLN-126 AND SER-156.
RC   STRAIN=168 / PY79;
RX   PubMed=16469701; DOI=10.1016/j.cell.2005.11.041;
RA   Ellermeier C.D., Hobbs E.C., Gonzalez-Pastor J.E., Losick R.;
RT   "A three-protein signaling pathway governing immunity to a bacterial
RT   cannibalism toxin.";
RL   Cell 124:549-559(2006).
CC   -!- FUNCTION: Immunity protein that provides protection for the cell
CC       against the toxic effects of its own sdpC killing factor, and that
CC       functions as a receptor/signal transduction protein as well. Once
CC       sdpC accumulates in the extracellular milieu, sdpI binds to sdpC,
CC       causing sequestration of sdpR at the bacterial membrane.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC       (Potential).
CC   -!- INDUCTION: By spo0A during nutrient starvation, through its direct
CC       negative control of abrB. Repressed by abrB during regular growth
CC       when nutrients are plentiful, and also by sdpR.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=I'll have you for
CC       supper - Issue 90 of January 2008;
CC       URL="http://www.expasy.org/spotlight/back_issues/sptlt090.shtml";
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DR   EMBL; AL009126; CAB15383.1; -; Genomic_DNA.
DR   PIR; C70029; C70029.
DR   RefSeq; NP_391258.1; -.
DR   TCDB; 9.A.32.1.1; SdpC (Peptide-antibiotic killer Factor) immunity protein, SdpI family.
DR   GeneID; 936238; -.
DR   GenomeReviews; AL009126_GR; BSU33780.
DR   KEGG; bsu:BSU33780; -.
DR   NMPDR; fig|224308.1.peg.3384; -.
DR   SubtiList; BG14077; sdpI.
DR   HOGENOM; O32241; -.
DR   OMA; O32241; WIFTFAI.
DR   BioCyc; BSUB224308:BSU3375-MON; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR012867; DUF1648.
DR   Pfam; PF07853; DUF1648; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Membrane; Transmembrane.
FT   CHAIN         1    207       Immunity protein sdpI.
FT                                /FTId=PRO_0000312744.
FT   TRANSMEM      5     25       Potential.
FT   TRANSMEM     47     67       Potential.
FT   TRANSMEM     78     98       Potential.
FT   TRANSMEM    100    120       Potential.
FT   TRANSMEM    150    170       Potential.
FT   TRANSMEM    171    191       Potential.
FT   MUTAGEN       6      6       I->T: Ability to bypass the requirement
FT                                of sdpC binding for expression of the
FT                                sdpRI genes.
FT   MUTAGEN      50     50       I->T: Ability to bypass the requirement
FT                                of sdpC binding for expression of the
FT                                sdpRI genes.
FT   MUTAGEN      78     78       F->I: Ability to bypass the requirement
FT                                of sdpC binding for expression of the
FT                                sdpRI genes.
FT   MUTAGEN      84     84       M->T: Ability to bypass the requirement
FT                                of sdpC binding for expression of the
FT                                sdpRI genes.
FT   MUTAGEN      85     85       L->S: Ability to bypass the requirement
FT                                of sdpC binding for expression of the
FT                                sdpRI genes.
FT   MUTAGEN      98     98       I->K: Ability to bypass the requirement
FT                                of sdpC binding for expression of the
FT                                sdpRI genes.
FT   MUTAGEN     126    126       Q->L: Loss of ability to promote
FT                                transcription of the sdpRI genes in
FT                                response to sdpC binding. No effect in
FT                                ability to confer immunity to sdpC.
FT   MUTAGEN     156    156       S->T: Loss of ability to promote
FT                                transcription of the sdpRI genes in
FT                                response to sdpC binding. No effect in
FT                                ability to confer immunity to sdpC.
SQ   SEQUENCE   207 AA;  23293 MW;  27171B056BA86490 CRC64;
     MKKNIISIII VCLSFLTSII LYQYLPEEIP IQWSGNKPAA IVSKPLTIFI IPVVMLIYYL
     TFYMLTIKST QKNKALLFLA SNNMLILLYI LQLSTLLISL GYEVNIDLII GLGVGIFLII
     GGNSMQLAEQ NHLIGLRTPW TLKDETVWKL GNRFASKVLV VCGFIIAVLS FFTGEYIILI
     MIVLVLLALV ISTLASYHYY KKLNGSR
//