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Protein

Immunity protein SdpI

Gene

sdpI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Immunity protein that provides protection for the cell against the toxic effects of SDP, its own SdpC-derived killing factor, and that functions as a receptor/signal transduction protein as well. Once SDP accumulates in the extracellular milieu, SdpI binds to SDP, causing sequestration of SdpR at the bacterial membrane.2 Publications

GO - Biological processi

  • response to toxic substance Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciBSUB:BSU33780-MONOMER.

Protein family/group databases

TCDBi9.A.32.1.1. the sdpc (peptide-antibiotic killer factor) immunity protein, sdpi (sdpi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Immunity protein SdpI
Gene namesi
Name:sdpI
Synonyms:yvaZ
Ordered Locus Names:BSU33780
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21
Transmembranei47 – 67HelicalSequence analysisAdd BLAST21
Transmembranei78 – 98HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Results in increased SigW expression which is dependent on SdpC. Results in weaker growth (70% of wild-type cell density) on LB medium, with more pronounced effects on sporulation medium (15% of wild-type). Concomitant deletion of SigW results in greatly diminished growth (5% of wild-type on LB and 3% on sporulation medium). Over time, single SdpI mutant stops growing on sporulation medium, while the SdpI SigW double mutant eventually lyses. Deletion of RsiW restores growth of the SdpI single mutant to near wild-type levels.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6I → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication1
Mutagenesisi50I → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication1
Mutagenesisi78F → I: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication1
Mutagenesisi84M → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication1
Mutagenesisi85L → S: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication1
Mutagenesisi98I → K: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication1
Mutagenesisi126Q → L: Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC. 1 Publication1
Mutagenesisi156S → T: Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003127441 – 207Immunity protein SdpIAdd BLAST207

Proteomic databases

PaxDbiO32241.

Expressioni

Inductioni

By Spo0A during nutrient starvation, through its direct negative control of AbrB, repressed by AbrB during regular growth when nutrients are plentiful, and also by SdpR (PubMed:16469701). Induced by expression of SDP (active peptide of sdpC) (PubMed:16469701, PubMed:23687264).2 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018326.

Structurei

3D structure databases

ProteinModelPortaliO32241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5658. LUCA.
InParanoidiO32241.
OMAiRFASRVF.

Family and domain databases

InterProiIPR012867. DUF1648.
IPR026272. SdpI.
IPR025962. SdpI/YhfL.
[Graphical view]
PfamiPF07853. DUF1648. 1 hit.
PF13630. SdpI. 1 hit.
[Graphical view]
PIRSFiPIRSF038959. SdpI. 1 hit.

Sequencei

Sequence statusi: Complete.

O32241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNIISIII VCLSFLTSII LYQYLPEEIP IQWSGNKPAA IVSKPLTIFI
60 70 80 90 100
IPVVMLIYYL TFYMLTIKST QKNKALLFLA SNNMLILLYI LQLSTLLISL
110 120 130 140 150
GYEVNIDLII GLGVGIFLII GGNSMQLAEQ NHLIGLRTPW TLKDETVWKL
160 170 180 190 200
GNRFASKVLV VCGFIIAVLS FFTGEYIILI MIVLVLLALV ISTLASYHYY

KKLNGSR
Length:207
Mass (Da):23,293
Last modified:January 1, 1998 - v1
Checksum:i27171B056BA86490
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15383.1.
PIRiC70029.
RefSeqiNP_391258.1. NC_000964.3.
WP_003228357.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15383; CAB15383; BSU33780.
GeneIDi936238.
KEGGibsu:BSU33780.
PATRICi18978756. VBIBacSub10457_3541.

Cross-referencesi

Web resourcesi

Protein Spotlight

I'll have you for supper - Issue 90 of January 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15383.1.
PIRiC70029.
RefSeqiNP_391258.1. NC_000964.3.
WP_003228357.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliO32241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018326.

Protein family/group databases

TCDBi9.A.32.1.1. the sdpc (peptide-antibiotic killer factor) immunity protein, sdpi (sdpi) family.

Proteomic databases

PaxDbiO32241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15383; CAB15383; BSU33780.
GeneIDi936238.
KEGGibsu:BSU33780.
PATRICi18978756. VBIBacSub10457_3541.

Phylogenomic databases

eggNOGiCOG5658. LUCA.
InParanoidiO32241.
OMAiRFASRVF.

Enzyme and pathway databases

BioCyciBSUB:BSU33780-MONOMER.

Family and domain databases

InterProiIPR012867. DUF1648.
IPR026272. SdpI.
IPR025962. SdpI/YhfL.
[Graphical view]
PfamiPF07853. DUF1648. 1 hit.
PF13630. SdpI. 1 hit.
[Graphical view]
PIRSFiPIRSF038959. SdpI. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSDPI_BACSU
AccessioniPrimary (citable) accession number: O32241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.