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Protein

Immunity protein SdpI

Gene

sdpI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Immunity protein that provides protection for the cell against the toxic effects of its own SdpC killing factor, and that functions as a receptor/signal transduction protein as well. Once SdpC accumulates in the extracellular milieu, SdpI binds to SdpC, causing sequestration of SdpR at the bacterial membrane.2 Publications

GO - Biological processi

  1. response to toxic substance Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciBSUB:BSU33780-MONOMER.

Protein family/group databases

TCDBi9.A.32.1.1. the sdpc (peptide-antibiotic killer factor) immunity protein, sdpi (sdpi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Immunity protein SdpI
Gene namesi
Name:sdpI
Synonyms:yvaZ
Ordered Locus Names:BSU33780
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU33780. [Micado]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2521HelicalSequence AnalysisAdd
BLAST
Transmembranei47 – 6721HelicalSequence AnalysisAdd
BLAST
Transmembranei78 – 9821HelicalSequence AnalysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Transmembranei171 – 19121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Results in increased SigW expression which is dependable on SdpC. Results in weaker growth (70% of wild-type cell density) on LB medium, with more pronounced effects on sporulation medium (15% of wild-type). Concomitant deletion of SigW results in greatly diminished growth (5% of wild-type on LB and 3% on sporulation medium). Over time, single SdpI mutant stops growing on sporulation medium, while the SdpI SigW double mutant eventually lyses. Deletion of RsiW restores growth of the SdpI single mutant to near wild-type levels.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi6 – 61I → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication
Mutagenesisi50 – 501I → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication
Mutagenesisi78 – 781F → I: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication
Mutagenesisi84 – 841M → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication
Mutagenesisi85 – 851L → S: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication
Mutagenesisi98 – 981I → K: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. 1 Publication
Mutagenesisi126 – 1261Q → L: Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC. 1 Publication
Mutagenesisi156 – 1561S → T: Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Immunity protein SdpIPRO_0000312744Add
BLAST

Proteomic databases

PaxDbiO32241.

Expressioni

Inductioni

By Spo0A during nutrient starvation, through its direct negative control of AbrB. Repressed by AbrB during regular growth when nutrients are plentiful, and also by SdpR.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU33780.

Structurei

3D structure databases

ProteinModelPortaliO32241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5658.
InParanoidiO32241.
OMAiRFASRVF.
OrthoDBiEOG63NMH6.

Family and domain databases

InterProiIPR012867. DUF1648.
IPR026272. SdpI.
IPR025962. SdpI/YhfL.
[Graphical view]
PfamiPF07853. DUF1648. 1 hit.
PF13630. SdpI. 1 hit.
[Graphical view]
PIRSFiPIRSF038959. SdpI. 1 hit.

Sequencei

Sequence statusi: Complete.

O32241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNIISIII VCLSFLTSII LYQYLPEEIP IQWSGNKPAA IVSKPLTIFI
60 70 80 90 100
IPVVMLIYYL TFYMLTIKST QKNKALLFLA SNNMLILLYI LQLSTLLISL
110 120 130 140 150
GYEVNIDLII GLGVGIFLII GGNSMQLAEQ NHLIGLRTPW TLKDETVWKL
160 170 180 190 200
GNRFASKVLV VCGFIIAVLS FFTGEYIILI MIVLVLLALV ISTLASYHYY

KKLNGSR
Length:207
Mass (Da):23,293
Last modified:January 1, 1998 - v1
Checksum:i27171B056BA86490
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15383.1.
PIRiC70029.
RefSeqiNP_391258.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15383; CAB15383; BSU33780.
GeneIDi936238.
KEGGibsu:BSU33780.
PATRICi18978756. VBIBacSub10457_3541.

Cross-referencesi

Web resourcesi

Protein Spotlight

I'll have you for supper - Issue 90 of January 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15383.1.
PIRiC70029.
RefSeqiNP_391258.1. NC_000964.3.

3D structure databases

ProteinModelPortaliO32241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU33780.

Protein family/group databases

TCDBi9.A.32.1.1. the sdpc (peptide-antibiotic killer factor) immunity protein, sdpi (sdpi) family.

Proteomic databases

PaxDbiO32241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15383; CAB15383; BSU33780.
GeneIDi936238.
KEGGibsu:BSU33780.
PATRICi18978756. VBIBacSub10457_3541.

Organism-specific databases

GenoListiBSU33780. [Micado]

Phylogenomic databases

eggNOGiCOG5658.
InParanoidiO32241.
OMAiRFASRVF.
OrthoDBiEOG63NMH6.

Enzyme and pathway databases

BioCyciBSUB:BSU33780-MONOMER.

Family and domain databases

InterProiIPR012867. DUF1648.
IPR026272. SdpI.
IPR025962. SdpI/YhfL.
[Graphical view]
PfamiPF07853. DUF1648. 1 hit.
PF13630. SdpI. 1 hit.
[Graphical view]
PIRSFiPIRSF038959. SdpI. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  2. "A three-protein signaling pathway governing immunity to a bacterial cannibalism toxin."
    Ellermeier C.D., Hobbs E.C., Gonzalez-Pastor J.E., Losick R.
    Cell 124:549-559(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ILE-6; ILE-50; PHE-78; MET-84; LEU-85; ILE-98; GLN-126 AND SER-156.
    Strain: 168 / PY79.
  3. "Identification of Bacillus subtilis sigma-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli."
    Butcher B.G., Helmann J.D.
    Mol. Microbiol. 60:765-782(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. "The Bacillus subtilis cannibalism toxin SDP collapses the proton motive force and induces autolysis."
    Lamsa A., Liu W.T., Dorrestein P.C., Pogliano K.
    Mol. Microbiol. 84:486-500(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 1681 Publication.

Entry informationi

Entry nameiSDPI_BACSU
AccessioniPrimary (citable) accession number: O32241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.