O32241 (SDPI_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Immunity protein SdpI

Gene names

Name:	sdpI
Synonyms:	yvaZ
Ordered Locus Names:	BSU33780

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Immunity protein that provides protection for the cell against the toxic effects of its own SdpC killing factor, and that functions as a receptor/signal transduction protein as well. Once SdpC accumulates in the extracellular milieu, SdpI binds to SdpC, causing sequestration of SdpR at the bacterial membrane. Ref.2
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	By Spo0A during nutrient starvation, through its direct negative control of AbrB. Repressed by AbrB during regular growth when nutrients are plentiful, and also by SdpR. Ref.2

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Immunity protein SdpI		PRO_0000312744
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Transmembrane	78 – 98	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	171 – 191	21	Helical; Potential
Experimental info
	Mutagenesis	6	1	I → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. Ref.2
	Mutagenesis	50	1	I → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. Ref.2
	Mutagenesis	78	1	F → I: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. Ref.2
	Mutagenesis	84	1	M → T: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. Ref.2
	Mutagenesis	85	1	L → S: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. Ref.2
	Mutagenesis	98	1	I → K: Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes. Ref.2
	Mutagenesis	126	1	Q → L: Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC. Ref.2
	Mutagenesis	156	1	S → T: Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O32241 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 27171B056BA86490	FASTA	207	23,293
	10 20 30 40 50 60 MKKNIISIII VCLSFLTSII LYQYLPEEIP IQWSGNKPAA IVSKPLTIFI IPVVMLIYYL 70 80 90 100 110 120 TFYMLTIKST QKNKALLFLA SNNMLILLYI LQLSTLLISL GYEVNIDLII GLGVGIFLII 130 140 150 160 170 180 GGNSMQLAEQ NHLIGLRTPW TLKDETVWKL GNRFASKVLV VCGFIIAVLS FFTGEYIILI 190 200 MIVLVLLALV ISTLASYHYY KKLNGSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"A three-protein signaling pathway governing immunity to a bacterial cannibalism toxin." Ellermeier C.D., Hobbs E.C., Gonzalez-Pastor J.E., Losick R. Cell 124:549-559(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ILE-6; ILE-50; PHE-78; MET-84; LEU-85; ILE-98; GLN-126 AND SER-156. Strain: 168 / PY79.

Web resources

Protein Spotlight

I'll have you for supper - Issue 90 of January 2008

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL009126 Genomic DNA. Translation: CAB15383.1.
PIR	C70029.
RefSeq	NP_391258.1. NC_000964.3.
3D structure databases
ProteinModelPortal	O32241.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU33780.
Protein family/group databases
TCDB	9.A.32.1.1. SdpC (Peptide-antibiotic killer Factor) immunity protein, SdpI family.
Proteomic databases
PaxDb	O32241.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB15383; CAB15383; BSU33780.
GeneID	936238.
KEGG	bsu:BSU33780.
PATRIC	18978756. VBIBacSub10457_3541.
Organism-specific databases
GenoList	BSU33780. [Micado]
Phylogenomic databases
eggNOG	COG5658.
OMA	PESMATH.
Enzyme and pathway databases
BioCyc	BSUB:BSU33780-MONOMER.
Family and domain databases
InterPro	IPR012867. DUF1648. IPR026272. SdpI. IPR025962. SdpI/YhfL. [Graphical view]
Pfam	PF07853. DUF1648. 1 hit. PF13630. SdpI. 1 hit. [Graphical view]
PIRSF	PIRSF038959. SdpI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SDPI_BACSU

Accession

Primary (citable) accession number: O32241

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program