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Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component (Probable).1 Publication

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), Sulfite reductase [NADPH] hemoprotein beta-component (cysI)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei495NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 78FMNPROSITE-ProRule annotation5
Nucleotide bindingi121 – 126FMNPROSITE-ProRule annotation6
Nucleotide bindingi154 – 185FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi392 – 395FADBy similarity4
Nucleotide bindingi525 – 533NADPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBSUB:BSU33440-MONOMER
UniPathwayiUPA00140; UER00207

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-component (EC:1.8.1.2)
Short name:
SiR-FP
Gene namesi
Name:cysJ
Synonyms:yvgR
Ordered Locus Names:BSU33440
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking the cysIJ genes are unable to use sulfate, sulfite or butanesulfonate as sole sulfur source, grow poorly with sulfide, but can still grow with thiosulfate, cysteine or methionine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003884691 – 605Sulfite reductase [NADPH] flavoprotein alpha-componentAdd BLAST605

Proteomic databases

PaxDbiO32214
PRIDEiO32214

Expressioni

Inductioni

Up-regulated by sulfate and the transcriptional regulator CysL.2 Publications

Interactioni

Subunit structurei

Alpha8-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018156

Structurei

3D structure databases

ProteinModelPortaliO32214
SMRiO32214
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 206Flavodoxin-likePROSITE-ProRule annotationAdd BLAST139
Domaini235 – 454FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST220

Phylogenomic databases

eggNOGiENOG4107EER Bacteria
COG0369 LUCA
HOGENOMiHOG000282025
InParanoidiO32214
KOiK00380
OMAiTKMDVAF
PhylomeDBiO32214

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
InterProiView protein in InterPro
IPR010199 CysJ
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000207 SiR-FP_CysJ, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR01931 cysJ, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

O32214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLQVMNSPF NQEQAELLNR LLPTLTESQK IWLSGYLSAQ SVSAQEAAGT
60 70 80 90 100
PAAAVSAEAP APAVSKEVTV LYGSQTGNAQ GLAENAGKQL EQSGFQVTVS
110 120 130 140 150
SMSDFKPNQL KKVTNLLIVV STHGEGEPPD NALSFHEFLH GRRAPKLEDL
160 170 180 190 200
RFSVLALGDS SYEFFCQTGK EFDQRLEELG GKRISPRVDC DLDYDEPAAE
210 220 230 240 250
WLEGVLKGLN EAGGGSAAPA PAAASQTGES SYSRTNPFRA EVLENLNLNG
260 270 280 290 300
RGSNKETRHV ELSLEGSGLT YEPGDSLGVY PENDPELVEL LLKEMNWDPE
310 320 330 340 350
EIVTLNKQGD VRPLKEALIS HYEITVLTKP LLEQAAQLTG NDELRELLAP
360 370 380 390 400
GNEENVKAYI EGRDLLDLVR DYGPFSVSAQ EFVSILRKMP ARLYSIASSL
410 420 430 440 450
SANPDEVHLT IGAVRYDAHG RERKGVCSIL CAERLQPGDT LPVYVQHNQN
460 470 480 490 500
FKLPKDPETP IIMVGPGTGV APFRSFMQER EETGAEGKAW MFFGDQHFVT
510 520 530 540 550
DFLYQTEWQN WLKDGVLTKM DVAFSRDTEE KVYVQHRMLE HSAELFEWLQ
560 570 580 590 600
EGAAVYICGD EKHMAHDVHN TLLEIIEKEG NMSREEAEAY LADMQQQKRY

QRDVY
Length:605
Mass (Da):67,259
Last modified:January 1, 1998 - v1
Checksum:i486F512C0AED6217
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15349.1
PIRiG70040
RefSeqiNP_391224.1, NC_000964.3
WP_003243259.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15349; CAB15349; BSU33440
GeneIDi936022
KEGGibsu:BSU33440
PATRICifig|224308.179.peg.3629

Similar proteinsi

Entry informationi

Entry nameiCYSJ_BACSU
AccessioniPrimary (citable) accession number: O32214
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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