Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate (Probable).1 Publication

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.

Cofactori

Protein has several cofactor binding sites:
  • sirohemeBy similarityNote: Binds 1 siroheme per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), Sulfite reductase [NADPH] hemoprotein beta-component (cysI)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron-sulfur (4Fe-4S)By similarity
Metal bindingi442 – 4421Iron-sulfur (4Fe-4S)By similarity
Metal bindingi481 – 4811Iron-sulfur (4Fe-4S)By similarity
Metal bindingi485 – 4851Iron (siroheme axial ligand)By similarity
Metal bindingi485 – 4851Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciBSUB:BSU33430-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-component (EC:1.8.1.2)
Short name:
SiR-HP
Short name:
SiRHP
Gene namesi
Name:cysI
Synonyms:yvgQ
Ordered Locus Names:BSU33430
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking the cysIJ genes are unable to use sulfate, sulfite or butanesulfonate as sole sulfur source, grow poorly with sulfide, but can still grow with thiosulfate, cysteine or methionine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Sulfite reductase [NADPH] hemoprotein beta-componentPRO_0000388478Add
BLAST

Proteomic databases

PaxDbiO32213.

Expressioni

Inductioni

Up-regulated by sulfate and the transcriptional regulator CysL.2 Publications

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).By similarity

Protein-protein interaction databases

IntActiO32213. 1 interaction.
MINTiMINT-8366664.
STRINGi224308.Bsubs1_010100018151.

Structurei

3D structure databases

ProteinModelPortaliO32213.
SMRiO32213. Positions 79-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105ET1. Bacteria.
COG0155. LUCA.
HOGENOMiHOG000218418.
InParanoidiO32213.
KOiK00381.
OMAiGKYNMYL.
PhylomeDBiO32213.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI. 1 hit.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTKILKAPD GSPSDVERIK KESDYLRGTL KEVMLDRISA GIPDDDNRLM
60 70 80 90 100
KHHGSYLQDD RDLRNERQKQ KLEPAYQFML RVRMPGGVST PEQWLVMDDL
110 120 130 140 150
SQKYGNGTLK LTTRETFQMH GILKWNMKKT IQTIHSALLD TIAACGDVNR
160 170 180 190 200
NVMCASNPYQ SEIHSEVYEW SKKLSDDLLP RTRAYHEIWL DEERVAGTPE
210 220 230 240 250
EEVEPMYGPL YLPRKFKIGI AVPPSNDIDV FSQDLGFIAI VEDGKLIGFN
260 270 280 290 300
VAIGGGMGMT HGDTATYPQL AKVIGFCRPE QMYDVAEKTI TIQRDYGNRS
310 320 330 340 350
VRKNARFKYT VDRLGLENVK EELENRLGWS LEEAKPYHFD HNGDRYGWVE
360 370 380 390 400
GIEDKWHFTL FVEGGRITDY DDYKLMTGLR EIAKVHTGEF RLTANQNLMI
410 420 430 440 450
ANVSSDKKEE ISALIEQYGL TDGKHYSALR RSSMACVALP TCGLAMAEAE
460 470 480 490 500
RYLPTLLDKI EEIIDENGLR DQEITIRMTG CPNGCARHAL GEIGFIGKAP
510 520 530 540 550
GKYNMYLGAA FDGSRLSKMY RENIGEADIL SELRILLSRY AKEREEGEHF
560 570
GDFVIRAGII KATTDGTNFH D
Length:571
Mass (Da):64,810
Last modified:January 1, 1998 - v1
Checksum:iE9A695C9797D6564
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15348.1.
PIRiF70040.
RefSeqiNP_391223.1. NC_000964.3.
WP_003243849.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15348; CAB15348; BSU33430.
GeneIDi938605.
KEGGibsu:BSU33430.
PATRICi18978686. VBIBacSub10457_3506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15348.1.
PIRiF70040.
RefSeqiNP_391223.1. NC_000964.3.
WP_003243849.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliO32213.
SMRiO32213. Positions 79-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO32213. 1 interaction.
MINTiMINT-8366664.
STRINGi224308.Bsubs1_010100018151.

Proteomic databases

PaxDbiO32213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15348; CAB15348; BSU33430.
GeneIDi938605.
KEGGibsu:BSU33430.
PATRICi18978686. VBIBacSub10457_3506.

Phylogenomic databases

eggNOGiENOG4105ET1. Bacteria.
COG0155. LUCA.
HOGENOMiHOG000218418.
InParanoidiO32213.
KOiK00381.
OMAiGKYNMYL.
PhylomeDBiO32213.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.
BioCyciBSUB:BSU33430-MONOMER.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI. 1 hit.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSI_BACSU
AccessioniPrimary (citable) accession number: O32213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.