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Protein

Sensor histidine kinase LiaS

Gene

liaS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Member of the two-component regulatory system LiaS/LiaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also involved in response to cationic antimicrobial peptides and secretion stress. Activates probably LiaR by phosphorylation.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU33090-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase LiaS (EC:2.7.13.3)
Gene namesi
Name:liaS
Synonyms:yvqE
Ordered Locus Names:BSU33090
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 47ExtracellularSequence analysisAdd BLAST11
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Topological domaini69 – 360CytoplasmicSequence analysisAdd BLAST292

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747801 – 360Sensor histidine kinase LiaSAdd BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei159Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO32198.
PRIDEiO32198.

Expressioni

Inductioni

Induced, via LiaR, by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and severe secretion stress.4 Publications

Interactioni

Protein-protein interaction databases

IntActiO32198. 4 interactors.
STRINGi224308.Bsubs1_010100017961.

Structurei

3D structure databases

ProteinModelPortaliO32198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 126HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini153 – 346Histidine kinasePROSITE-ProRule annotationAdd BLAST194

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ENE. Bacteria.
COG4585. LUCA.
HOGENOMiHOG000279747.
InParanoidiO32198.
KOiK11617.
OMAiNEQITEF.
PhylomeDBiO32198.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR017202. LiaS/VraS.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037431. STHK_LiaS. 1 hit.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32198-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKKMLASLQ WRAIRMTTGI SLLLFVCLIS FMMFYYRLDP LVLLSSSWFG
60 70 80 90 100
IPFILILLLI SVTVGFASGY MYGNRLKTRI DTLIESILTF ENGNFAYRIP
110 120 130 140 150
PLGDDEIGLA ADQLNEMAKR VELQVASLQK LSNERAEWQA QMKKSVISEE
160 170 180 190 200
RQRLARDLHD AVSQQLFAIS MMTSAVLEHV KDADDKTVKR IRMVEHMAGE
210 220 230 240 250
AQNEMRALLL HLRPVTLEGK GLKEGLTELL DEFRKKQPID IEWDIQDTAI
260 270 280 290 300
SKGVEDHLFR IVQEALSNVF RHSKASKVTV ILGIKNSQLR LKVIDNGKGF
310 320 330 340 350
KMDQVKASSY GLNSMKERAS EIGGVAEVIS VEGKGTQIEV KVPIFPEEKG
360
ENERDSSIID
Length:360
Mass (Da):40,704
Last modified:January 1, 1998 - v1
Checksum:iB2DEE6E018B7AC7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223978 Genomic DNA. Translation: CAA11744.1.
AL009126 Genomic DNA. Translation: CAB15299.1.
PIRiF70045.
RefSeqiNP_391189.1. NC_000964.3.
WP_003244464.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15299; CAB15299; BSU33090.
GeneIDi935967.
KEGGibsu:BSU33090.
PATRICi18978606. VBIBacSub10457_3466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223978 Genomic DNA. Translation: CAA11744.1.
AL009126 Genomic DNA. Translation: CAB15299.1.
PIRiF70045.
RefSeqiNP_391189.1. NC_000964.3.
WP_003244464.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliO32198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO32198. 4 interactors.
STRINGi224308.Bsubs1_010100017961.

Proteomic databases

PaxDbiO32198.
PRIDEiO32198.

Protocols and materials databases

DNASUi935967.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15299; CAB15299; BSU33090.
GeneIDi935967.
KEGGibsu:BSU33090.
PATRICi18978606. VBIBacSub10457_3466.

Phylogenomic databases

eggNOGiENOG4105ENE. Bacteria.
COG4585. LUCA.
HOGENOMiHOG000279747.
InParanoidiO32198.
KOiK11617.
OMAiNEQITEF.
PhylomeDBiO32198.

Enzyme and pathway databases

BioCyciBSUB:BSU33090-MONOMER.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR017202. LiaS/VraS.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037431. STHK_LiaS. 1 hit.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIAS_BACSU
AccessioniPrimary (citable) accession number: O32198
Secondary accession number(s): Q7B2J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.