Reviewed, UniProtKB/Swiss-Prot O32179 (PROD1_BACSU)

Names and origin

Protein names

Recommended name:
    Proline dehydrogenase 1
      Short name=PRODH 1
    EC=1.5.99.8
Alternative name(s):
    Proline oxidase 1

Gene names

Name:	fadM
Synonyms:	yusM
Ordered Locus Names:	BSU32850

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidation of L-proline to delta-1-pyrroline-5-carboxylate (P5C) By similarity.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Induction	Repressed by fadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize fadR as to its binding to fadR boxes on target DNA and thus derepress transcription. Ref.2
Sequence similarities	Belongs to the proline dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	proline dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Proline dehydrogenase 1		PRO_0000360508

Sequences

	Sequence		Length	Mass (Da)
	O32179-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7606141E776B4A2B	FASTA	302	34,452
	10 20 30 40 50 60 MLRHVFLFLS QNKTLTKFAK AYGTRLGARR FVAGDTIESA VKTVKRLNRS GLCATIDYLG 70 80 90 100 110 120 EYAASEKEAN QVAEECKKAI QAIAEHQLNS ELSLKLTSIG LDLSEELALT HLRAILSVAK 130 140 150 160 170 180 QYDVAVTIDM EDYSHYEQTL SIYRQCKQEF EKLGTVIQAY LYRAAEDIRK MRDLKPNLRL 190 200 210 220 230 240 VKGAYKESAA VAFPDKRGTD LHFQSLIKLQ LLSGNYTAVA THDDDIIAFT KQLVAEHQIP 250 260 270 280 290 300 ASQFEFQMLY GIRPERQKEL AKEGYRMRVY VPYGTDWFSY FMRRIAERPA NAAFVLKGIL KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation." Matsuoka H., Hirooka K., Fujita Y. J. Biol. Chem. 282:5180-5194(2007) [PubMed: 17189250] [Abstract] Cited for: GENE NAME, INDUCTION. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB15274.1.
PIR	F70021.
RefSeq	NP_391164.1.
3D structure databases
HSSP	HSSP built from PDB template 1K87 based on UniProtKB P09546.
ModBase	Search...
Genome annotation databases
GeneID	937064.
GenomeReviews	Gene locus BSU32850 in contig AL009126_GR.
KEGG	bsu:BSU32850.
NMPDR	fig|224308.1.peg.3290.
Organism-specific databases
SubtiList	BG14025. fadM. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O32179.
OMA	VAFPDKR.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3281-MON.
Family and domain databases
InterPro	IPR015590. Aldehyde_DH. IPR002872. Proline_DH. IPR008219. Proline_DH_pred. [Graphical view]
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000196. Pro_dehydrog. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PROD1_BACSU

Accession

Primary (citable) accession number: O32179

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)