O32178 (FADN_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-hydroxyacyl-CoA dehydrogenase
EC=1.1.1.35

Gene names

Name:	fadN
Synonyms:	yusL
Ordered Locus Names:	BSU32840

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	789 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the degradation of long-chain fatty acids.
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Induction	Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription. Ref.2
Sequence similarities	Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
Sequence caution	The sequence CAB15273.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid beta-oxidation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 789	789	Probable 3-hydroxyacyl-CoA dehydrogenase		PRO_0000360673

Sequences

	Sequence		Length	Mass (Da)
	O32178 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 9379318D7E70F40C	FASTA	789	87,085
	10 20 30 40 50 60 MHKHIRKAAV LGSGVMGSGI AAHLANIGIP VLLLDIVPND LTKEEEKKGL TKDSSEVRSR 70 80 90 100 110 120 LSRQAMKKLL KQKPAPLTSA KNTSYITPGN LEDDAEKLKE ADWIIEVVVE NLEVKKKIFA 130 140 150 160 170 180 LVDEHRKTGS IVSSNTSGIS VQEMAEGRSD DFKAHFLGTH FFNPARYLKL LEIIPIKETD 190 200 210 220 230 240 PDILKFMTAF GENVLGKGVV TAKDTPNFIA NRIGTYGLLV TVQEMLKGGY QVGEVDSITG 250 260 270 280 290 300 PLIGRPKSAT FRTLDVVGLD TFAHVARNVY DKADGDEKEV FRIPSFMNDM LEKGWIGSKA 310 320 330 340 350 360 GQGFYKKEGK TIYELDPVTL TYGERTKMKS PALEAAKQAK GTKAKMKALI YSDDRAGRLL 370 380 390 400 410 420 WNITSQTLLY SAELLGEIAD DIHAIDQAMK WGFGWELGPF EMWDAIGLKQ SAEKLEQLGA 430 440 450 460 470 480 DMPGWIKEML DKGNETFYIK ENGTVFYYDR GEYRAVKENK KRIHLQALKE TKGVIAKNSG 490 500 510 520 530 540 ASLIDLGDDV ALLEFHSKSN AIGLDIIQMI HKGLEETERN YKGLVIGNQG KNFCVGANLA 550 560 570 580 590 600 MILMEVQDDN FLEVDFVIRR FQETMMKIKY SAKPVVAAPF GMTLGGGTEA CLPAARIQAA 610 620 630 640 650 660 SEAYMGLVES GVGLIPGGGG NKELYINHLR RGHDPMNAAM KTFETIAMAK VSASAQEARE 670 680 690 700 710 720 MNILKETDQI SVNQDHLLYD AKQLAASLYD TGWRPPVKEK VKVPGETGYA ALLLGAEQMK 730 740 750 760 770 780 LSGYISEHDF KIAKKLAYVI AGGKVPFGTE VDEEYLLEIE REAFLSLSGE AKSQARMQHM LVKGKPLRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation." Matsuoka H., Hirooka K., Fujita Y. J. Biol. Chem. 282:5180-5194(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, GENE NAME, IDENTIFICATION OF INITIATION SITE. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL009126 Genomic DNA. Translation: CAB15273.1. Different initiation.
PIR	E70021.
3D structure databases
HSSP	HSSP built from PDB template 3HDH based on UniProtKB P00348.
ProteinModelPortal	O32178.
SMR	O32178. Positions 2-439, 483-787.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU32840.
Proteomic databases
PaxDb	O32178.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB15273; CAB15273; BSU32840.
KEGG	bsu:BSU32840.
PATRIC	18978548. VBIBacSub10457_3437.
Organism-specific databases
GenoList	BSU32840. [Micado]
Phylogenomic databases
eggNOG	COG1250.
HOGENOM	HOG000264370.
KO	K07516.
ProtClustDB	CLSK2460956.
Enzyme and pathway databases
BioCyc	BSUB:BSU32840-MONOMER.
UniPathway	UPA00659.
Family and domain databases
Gene3D	1.10.1040.10. 2 hits. 3.40.50.720. 1 hit.
InterPro	IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR001753. Crotonase_core_superfam. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00725. 3HCDH. 2 hits. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

FADN_BACSU

Accession

Primary (citable) accession number: O32178

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program