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O32178 (FADN_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 3-hydroxyacyl-CoA dehydrogenase

EC=1.1.1.35
Gene names
Name:fadN
Synonyms:yusL
Ordered Locus Names:BSU32840
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length789 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the degradation of long-chain fatty acids.

Catalytic activity

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

Pathway

Lipid metabolism; fatty acid beta-oxidation.

Induction

Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription. Ref.2

Sequence similarities

Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid degradation
Lipid metabolism
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfatty acid beta-oxidation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function3-hydroxyacyl-CoA dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

coenzyme binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 789789Probable 3-hydroxyacyl-CoA dehydrogenase
PRO_0000360673

Sequences

Sequence LengthMass (Da)Tools
O32178 [UniParc].

Last modified January 20, 2009. Version 2.
Checksum: 9379318D7E70F40C

FASTA78987,085
        10         20         30         40         50         60 
MHKHIRKAAV LGSGVMGSGI AAHLANIGIP VLLLDIVPND LTKEEEKKGL TKDSSEVRSR 

        70         80         90        100        110        120 
LSRQAMKKLL KQKPAPLTSA KNTSYITPGN LEDDAEKLKE ADWIIEVVVE NLEVKKKIFA 

       130        140        150        160        170        180 
LVDEHRKTGS IVSSNTSGIS VQEMAEGRSD DFKAHFLGTH FFNPARYLKL LEIIPIKETD 

       190        200        210        220        230        240 
PDILKFMTAF GENVLGKGVV TAKDTPNFIA NRIGTYGLLV TVQEMLKGGY QVGEVDSITG 

       250        260        270        280        290        300 
PLIGRPKSAT FRTLDVVGLD TFAHVARNVY DKADGDEKEV FRIPSFMNDM LEKGWIGSKA 

       310        320        330        340        350        360 
GQGFYKKEGK TIYELDPVTL TYGERTKMKS PALEAAKQAK GTKAKMKALI YSDDRAGRLL 

       370        380        390        400        410        420 
WNITSQTLLY SAELLGEIAD DIHAIDQAMK WGFGWELGPF EMWDAIGLKQ SAEKLEQLGA 

       430        440        450        460        470        480 
DMPGWIKEML DKGNETFYIK ENGTVFYYDR GEYRAVKENK KRIHLQALKE TKGVIAKNSG 

       490        500        510        520        530        540 
ASLIDLGDDV ALLEFHSKSN AIGLDIIQMI HKGLEETERN YKGLVIGNQG KNFCVGANLA 

       550        560        570        580        590        600 
MILMEVQDDN FLEVDFVIRR FQETMMKIKY SAKPVVAAPF GMTLGGGTEA CLPAARIQAA 

       610        620        630        640        650        660 
SEAYMGLVES GVGLIPGGGG NKELYINHLR RGHDPMNAAM KTFETIAMAK VSASAQEARE 

       670        680        690        700        710        720 
MNILKETDQI SVNQDHLLYD AKQLAASLYD TGWRPPVKEK VKVPGETGYA ALLLGAEQMK 

       730        740        750        760        770        780 
LSGYISEHDF KIAKKLAYVI AGGKVPFGTE VDEEYLLEIE REAFLSLSGE AKSQARMQHM 


LVKGKPLRN 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[2]"Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation."
Matsuoka H., Hirooka K., Fujita Y.
J. Biol. Chem. 282:5180-5194(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, GENE NAME, IDENTIFICATION OF INITIATION SITE.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL009126 Genomic DNA. Translation: CAB15273.2.
PIRE70021.

3D structure databases

ProteinModelPortalO32178.
SMRO32178. Positions 2-439, 483-787.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU32840.

Proteomic databases

PaxDbO32178.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15273; CAB15273; BSU32840.
PATRIC18978548. VBIBacSub10457_3437.

Organism-specific databases

GenoListBSU32840. [Micado]

Phylogenomic databases

eggNOGCOG1250.
HOGENOMHOG000264370.
OrthoDBEOG6B8XF5.

Enzyme and pathway databases

BioCycBSUB:BSU32840-MONOMER.
UniPathwayUPA00659.

Family and domain databases

Gene3D1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFADN_BACSU
AccessionPrimary (citable) accession number: O32178
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 20, 2009
Last modified: June 11, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList