O32167 (METQ_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine-binding lipoprotein MetQ

Gene names

Name:	metQ
Synonyms:	yusA
Ordered Locus Names:	BSU32730

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex MetNPQ involved in methionine import. Binds the methionine and transfers it to the membrane-bound permease. It has also been shown to be involved in methionine sulfoxide transport Probable. Ref.2
Subunit structure	The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetP) and a solute-binding protein (metQ) By similarity.
Subcellular location	Cell membrane; Lipid-anchor Probable.
Induction	Repressed by methionine via the S-box system. Ref.2
Sequence similarities	Belongs to the NlpA lipoprotein family.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 274

255

Methionine-binding lipoprotein MetQ

PRO_0000383645

Amino acid modifications

Lipidation

20

1

N-palmitoyl cysteine Potential

Lipidation

20

1

S-diacylglycerol cysteine Potential

Secondary structure

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274

Helix Strand Turn

Details...

Beta strand

32 – 37

6

Turn

39 – 41

3

Helix

42 – 54

13

Turn

55 – 57

3

Beta strand

59 – 63

5

Helix

70 – 76

7

Beta strand

81 – 87

7

Helix

88 – 97

10

Beta strand

103 – 111

9

Beta strand

115 – 117

3

Helix

124 – 126

3

Beta strand

132 – 136

5

Helix

139 – 141

3

Helix

142 – 151

10

Helix

163 – 165

3

Helix

168 – 170

3

Beta strand

171 – 173

3

Beta strand

179 – 183

5

Turn

185 – 187

3

Helix

188 – 193

6

Beta strand

197 – 202

6

Helix

204 – 209

6

Helix

214 – 217

4

Beta strand

219 – 221

3

Beta strand

231 – 236

6

Helix

243 – 252

10

Helix

255 – 264

10

Turn

265 – 267

3

Beta strand

268 – 271

4

Sequences

	Sequence		Length	Mass (Da)
	O32167 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3D40F949A1BFC73C	FASTA	274	30,355
	10 20 30 40 50 60 MKKLFLGALL LVFAGVMAAC GSNNGAESGK KEIVVAATKT PHAEILKEAE PLLKEKGYTL 70 80 90 100 110 120 KVKVLSDYKM YNKALADKEV DANYFQHIPY LEQEMKENTD YKLVNAGAVH LEPFGIYSKT 130 140 150 160 170 180 YKSLKDLPDG ATIILTNNVA EQGRMLAMLE NAGLITLDSK VETVDATLKD IKKNPKNLEF 190 200 210 220 230 240 KKVAPELTAK AYENKEGDAV FINVNYAIQN KLNPKKDAIE VESTKNNPYA NIIAVRKGEE 250 260 270 DSAKIKALME VLHSKKIKDF IEKKYDGAVL PVSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, D- and L-methionine." Hullo M.-F., Auger S., Dassa E., Danchin A., Martin-Verstraete I. Res. Microbiol. 155:80-86(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROBABLE FUNCTION IN METHIONINE AND METHIONINE SULFOXIDE TRANSPORT, INDUCTION. Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL009126 Genomic DNA. Translation: CAB15262.1.

PIR

B70020.

RefSeq

NP_391152.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GOT	X-ray	1.95	A	27-274	[»]

ProteinModelPortal

O32167.

SMR

O32167. Positions 32-274.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU32730.

Protein family/group databases

TCDB

3.A.1.24.2. ATP-binding cassette (ABC) superfamily.

Proteomic databases

PaxDb

O32167.

Protocols and materials databases

DNASU

936716.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB15262; CAB15262; BSU32730.

GeneID

936716.

KEGG

bsu:BSU32730.

PATRIC

18978526. VBIBacSub10457_3426.

Organism-specific databases

GenoList

BSU32730. [Micado]

Phylogenomic databases

eggNOG

COG1464.

HOGENOM

HOG000222927.

KO

K02073.

OMA

DAVFINV.

ProtClustDB

CLSK887840.

Enzyme and pathway databases

BioCyc

BSUB:BSU32730-MONOMER.

Family and domain databases

InterPro

IPR004872. Lipoprotein_NlpA.
[Graphical view]

Pfam

PF03180. Lipoprotein_9. 1 hit.
[Graphical view]

PIRSF

PIRSF002854. MetQ. 1 hit.

PROSITE

PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

METQ_BACSU

Accession

Primary (citable) accession number: O32167

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program