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Protein

Probable xanthine dehydrogenase subunit A

Gene

pucA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function.1 Publication

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Pathwayi

GO - Molecular functioni

  1. xanthine dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. hypoxanthine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBSUB:BSU32510-MONOMER.
UniPathwayiUPA00604; UER00661.
UPA00604; UER00662.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xanthine dehydrogenase subunit A (EC:1.17.1.4)
Short name:
XDHase subunit A
Gene namesi
Name:pucA
Synonyms:yurF
Ordered Locus Names:BSU32510
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU32510. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Probable xanthine dehydrogenase subunit APRO_0000166100Add
BLAST

Proteomic databases

PaxDbiO32147.

Expressioni

Inductioni

Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.

Interactioni

Subunit structurei

Could be composed of four subunits: PucA, PucC, PucD and PucE.

Protein-protein interaction databases

STRINGi224308.BSU32510.

Structurei

3D structure databases

ProteinModelPortaliO32147.
SMRiO32147. Positions 1-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1975.
HOGENOMiHOG000244666.
InParanoidiO32147.
KOiK00087.
OMAiWIGANGE.
OrthoDBiEOG680X47.
PhylomeDBiO32147.

Family and domain databases

InterProiIPR003777. XdhC_CoxI.
IPR027051. XdhC_Rossmann_dom.
[Graphical view]
PfamiPF13478. XdhC_C. 1 hit.
PF02625. XdhC_CoxI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNFHTMLDA LLEDQEEAVL ATIVQVEGSA YRKAGASMLF KKKGRRIGLL
60 70 80 90 100
SGGCVEEDVF QRISALGDQL TSTLIPYDMR SEDDLSWGMG AGCNGIIHVH
110 120 130 140 150
AERITQEKRR HYEKVRDCLH SGKAVTSVIK IESSHYLFLT ENGHFGNWPD
160 170 180 190 200
APLQDIQRTV STLHLPHFDQ TTNMFIQRIE PKPRLILFGA GPDNVPLANL
210 220 230 240 250
AADTGFSVIV TDWRPAYCTS SLFPKADQLI TAFPEQMLSE FQFFPHDAAV
260 270 280 290 300
VATHHYQHDQ TIINFLFSQN LHYIGLLGSA NRTKRLLSGK HPPSHFYSPV
310 320 330
GLKIGAEGPE EIAVSVVAEI IQTRKRVAVV
Length:330
Mass (Da):36,746
Last modified:December 31, 1997 - v1
Checksum:iE4A70B2155BE1902
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15241.1.
PIRiE70017.
RefSeqiNP_391131.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15241; CAB15241; BSU32510.
GeneIDi938865.
KEGGibsu:BSU32510.
PATRICi18978476. VBIBacSub10457_3401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15241.1.
PIRiE70017.
RefSeqiNP_391131.1. NC_000964.3.

3D structure databases

ProteinModelPortaliO32147.
SMRiO32147. Positions 1-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU32510.

Proteomic databases

PaxDbiO32147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15241; CAB15241; BSU32510.
GeneIDi938865.
KEGGibsu:BSU32510.
PATRICi18978476. VBIBacSub10457_3401.

Organism-specific databases

GenoListiBSU32510. [Micado]

Phylogenomic databases

eggNOGiCOG1975.
HOGENOMiHOG000244666.
InParanoidiO32147.
KOiK00087.
OMAiWIGANGE.
OrthoDBiEOG680X47.
PhylomeDBiO32147.

Enzyme and pathway databases

UniPathwayiUPA00604; UER00661.
UPA00604; UER00662.
BioCyciBSUB:BSU32510-MONOMER.

Family and domain databases

InterProiIPR003777. XdhC_CoxI.
IPR027051. XdhC_Rossmann_dom.
[Graphical view]
PfamiPF13478. XdhC_C. 1 hit.
PF02625. XdhC_CoxI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  2. "Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator."
    Schultz A.C., Nygaard P., Saxild H.H.
    J. Bacteriol. 183:3293-3302(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 168.

Entry informationi

Entry nameiXDHA_BACSU
AccessioniPrimary (citable) accession number: O32147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2002
Last sequence update: December 31, 1997
Last modified: January 6, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.