Reviewed, UniProtKB/Swiss-Prot O32147 (XDHA_BACSU)

Names and origin

Protein names

Recommended name:
    Probable xanthine dehydrogenase subunit A
      Short name=XDHase subunit A
    EC=1.17.1.4

Gene names

Name:	pucA
Synonyms:	yurF
Ordered Locus Names:	BSU32510

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function. Ref.2
Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Pathway	Purine metabolism; hypoxanthine degradation; uric acid from hypoxanthine: step 1/2. Purine metabolism; hypoxanthine degradation; uric acid from hypoxanthine: step 2/2.
Subunit structure	Could be composed of four subunits: pucA, pucC, pucD and pucE.
Induction	Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.

Ontologies

Keywords
Biological process	Purine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Probable xanthine dehydrogenase subunit A		PRO_0000166100

Sequences

	Sequence		Length	Mass (Da)
	O32147-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E4A70B2155BE1902	FASTA	330	36,746
	10 20 30 40 50 60 MGNFHTMLDA LLEDQEEAVL ATIVQVEGSA YRKAGASMLF KKKGRRIGLL SGGCVEEDVF 70 80 90 100 110 120 QRISALGDQL TSTLIPYDMR SEDDLSWGMG AGCNGIIHVH AERITQEKRR HYEKVRDCLH 130 140 150 160 170 180 SGKAVTSVIK IESSHYLFLT ENGHFGNWPD APLQDIQRTV STLHLPHFDQ TTNMFIQRIE 190 200 210 220 230 240 PKPRLILFGA GPDNVPLANL AADTGFSVIV TDWRPAYCTS SLFPKADQLI TAFPEQMLSE 250 260 270 280 290 300 FQFFPHDAAV VATHHYQHDQ TIINFLFSQN LHYIGLLGSA NRTKRLLSGK HPPSHFYSPV 310 320 330 GLKIGAEGPE EIAVSVVAEI IQTRKRVAVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator." Schultz A.C., Nygaard P., Saxild H.H. J. Bacteriol. 183:3293-3302(2001) [PubMed: 11344136] [Abstract] Cited for: FUNCTION. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB15241.1.
PIR	E70017.
RefSeq	NP_391131.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938865.
GenomeReviews	Gene locus BSU32510 in contig AL009126_GR.
KEGG	bsu:BSU32510.
NMPDR	fig|224308.1.peg.3257.
Organism-specific databases
SubtiList	BG13992. pucA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O32147.
OMA	O32147. EDDLSWG.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3248-MON.
BRENDA	1.17.1.4. 150.
Family and domain databases
InterPro	IPR003777. XdhC_CoxI. [Graphical view]
Pfam	PF02625. XdhC_CoxI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XDHA_BACSU

Accession

Primary (citable) accession number: O32147

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)