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Protein

Probable xanthine dehydrogenase subunit D

Gene

pucD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Oxidizes hypoxanthine and xanthine to uric acid.1 Publication

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Pathwayi: hypoxanthine degradation

This protein is involved in step 1 and 2 of the subpathway that synthesizes urate from hypoxanthine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable xanthine dehydrogenase subunit C (pucC), Probable xanthine dehydrogenase subunit A (pucA), Probable xanthine dehydrogenase subunit D (pucD), Probable xanthine dehydrogenase subunit E (pucE)
  2. Probable xanthine dehydrogenase subunit C (pucC), Probable xanthine dehydrogenase subunit A (pucA), Probable xanthine dehydrogenase subunit D (pucD), Probable xanthine dehydrogenase subunit E (pucE)
This subpathway is part of the pathway hypoxanthine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes urate from hypoxanthine, the pathway hypoxanthine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi204MolybdenumSequence analysis1
Metal bindingi235Molybdenum; via carbonyl oxygenSequence analysis1
Metal bindingi508Molybdenum; via amide nitrogenSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism
LigandMetal-binding, Molybdenum, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU32480-MONOMER
UniPathwayiUPA00604; UER00661
UPA00604; UER00662

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xanthine dehydrogenase subunit D (EC:1.17.1.4)
Short name:
XDHase subunit D
Gene namesi
Name:pucD
Synonyms:yurC
Ordered Locus Names:BSU32480
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660981 – 745Probable xanthine dehydrogenase subunit DAdd BLAST745

Proteomic databases

PaxDbiO32144
PRIDEiO32144

Expressioni

Inductioni

Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.

Interactioni

Subunit structurei

Could be composed of four subunits: PucA, PucC, PucD and PucE.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017621

Structurei

3D structure databases

ProteinModelPortaliO32144
SMRiO32144
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105CE2 Bacteria
COG1529 LUCA
HOGENOMiHOG000244715
InParanoidiO32144
KOiK00087
OMAiPYNPVAP
PhylomeDBiO32144

Family and domain databases

InterProiView protein in InterPro
IPR000674 Ald_Oxase/Xan_DH_a/b
IPR036856 Ald_Oxase/Xan_DH_a/b_sf
IPR008274 AldOxase/xan_DH_Mopterin-bd
IPR037165 AldOxase/xan_DH_Mopterin-bd_sf
IPR017609 Xanthine_dehydrogenase_dsu
PfamiView protein in Pfam
PF01315 Ald_Xan_dh_C, 1 hit
PF02738 Ald_Xan_dh_C2, 1 hit
SMARTiView protein in SMART
SM01008 Ald_Xan_dh_C, 1 hit
SUPFAMiSSF54665 SSF54665, 1 hit
SSF56003 SSF56003, 1 hit
TIGRFAMsiTIGR03196 pucD, 1 hit

Sequencei

Sequence statusi: Complete.

O32144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIINKPSRVR PDGRGKVTGE LKYMTDLSFP GMLYGKVLRS AYPHAEIVSV
60 70 80 90 100
CTIKAEKMEG VQAVVTHKDV PGLNRFGIVI PDQPVLCEDR VRYVGDAIAA
110 120 130 140 150
VAAETEEIAE AALELIQVEY KELEVMDSPE KALRPNAQRL HEDGNILHRA
160 170 180 190 200
FFSNGDVEEG FQASDTVFEE TYELPRQMHT YMETEGGVAV PEDDGGFTMY
210 220 230 240 250
AGTQHGYKDR FQLARIFDIP EEKIRIVSSP MGGSFGGKDE LNIQPYAALL
260 270 280 290 300
ALKSGRPVKI HQTRKESVRS GIKRHPMKIT IKTGADHSGN LLAHDVKIVA
310 320 330 340 350
DTGAYATLGP AVLDFSVEHA AGPYRIPNIR TEGISVFTNN GVAGEFRGFG
360 370 380 390 400
GNQITFALET HLDRLSGMLG IDPLELRRKN IRKPHDLGPL EHRIAPTDGA
410 420 430 440 450
AQVLNAISKS PILKKTSRNC GYLQRGTGAA ITMHGGGLGF GRMDAAGGRL
460 470 480 490 500
SLSSEGKITA SFGFEECGQG ILAAIEQIVM EELGCAAEDI SIVIGDTAKV
510 520 530 540 550
PKSGSSTASR GTSMVWHAIQ RLKKPFLAQL KKRAAEWSGC SAENLIPGAA
560 570 580 590 600
GLRDKNTKAL VVTYKELAEK GPLAEETAFD FPTTPDPVVG GHFLYSFGAA
610 620 630 640 650
AVEVEVDLLT GDVKLIDCEH AIAAGPVVSP QGYRGQIEGG AAMALGYTLM
660 670 680 690 700
EEAKMTDGRY AAENLDHYLI PGIKDVPDMK LIAIEDLMKG DVYGPRGVGE
710 720 730 740
IGTIAITPAI VKAVHDAVGC WINKLPISRE ELLEAIDRKG LKQWT
Length:745
Mass (Da):80,434
Last modified:January 1, 1998 - v1
Checksum:iC4A3EC5F0094EF8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15238.1
PIRiB70017
RefSeqiNP_391128.1, NC_000964.3
WP_003243174.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15238; CAB15238; BSU32480
GeneIDi936686
KEGGibsu:BSU32480
PATRICifig|224308.179.peg.3517

Entry informationi

Entry nameiXDHD_BACSU
AccessioniPrimary (citable) accession number: O32144
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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