Reviewed, UniProtKB/Swiss-Prot O32144 (XDHD_BACSU)

Names and origin

Protein names

Recommended name:
    Probable xanthine dehydrogenase subunit D
      Short name=XDHase subunit D
    EC=1.17.1.4

Gene names

Name:	pucD
Synonyms:	yurC
Ordered Locus Names:	BSU32480

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidizes hypoxanthine and xanthine to uric acid. Ref.2
Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	Molybdopterin By similarity.
Pathway	Purine metabolism; hypoxanthine degradation; uric acid from hypoxanthine: step 1/2. Purine metabolism; hypoxanthine degradation; uric acid from hypoxanthine: step 2/2.
Subunit structure	Could be composed of four subunits: pucA, pucC, pucD and pucE.
Induction	Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.
Sequence similarities	Belongs to the xanthine dehydrogenase family.

Ontologies

Keywords
Biological process	Purine metabolism
Ligand	Molybdenum NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	Probable xanthine dehydrogenase subunit D		PRO_0000166098

Sequences

	Sequence		Length	Mass (Da)
	O32144-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C4A3EC5F0094EF8D	FASTA	745	80,434
	10 20 30 40 50 60 MIINKPSRVR PDGRGKVTGE LKYMTDLSFP GMLYGKVLRS AYPHAEIVSV CTIKAEKMEG 70 80 90 100 110 120 VQAVVTHKDV PGLNRFGIVI PDQPVLCEDR VRYVGDAIAA VAAETEEIAE AALELIQVEY 130 140 150 160 170 180 KELEVMDSPE KALRPNAQRL HEDGNILHRA FFSNGDVEEG FQASDTVFEE TYELPRQMHT 190 200 210 220 230 240 YMETEGGVAV PEDDGGFTMY AGTQHGYKDR FQLARIFDIP EEKIRIVSSP MGGSFGGKDE 250 260 270 280 290 300 LNIQPYAALL ALKSGRPVKI HQTRKESVRS GIKRHPMKIT IKTGADHSGN LLAHDVKIVA 310 320 330 340 350 360 DTGAYATLGP AVLDFSVEHA AGPYRIPNIR TEGISVFTNN GVAGEFRGFG GNQITFALET 370 380 390 400 410 420 HLDRLSGMLG IDPLELRRKN IRKPHDLGPL EHRIAPTDGA AQVLNAISKS PILKKTSRNC 430 440 450 460 470 480 GYLQRGTGAA ITMHGGGLGF GRMDAAGGRL SLSSEGKITA SFGFEECGQG ILAAIEQIVM 490 500 510 520 530 540 EELGCAAEDI SIVIGDTAKV PKSGSSTASR GTSMVWHAIQ RLKKPFLAQL KKRAAEWSGC 550 560 570 580 590 600 SAENLIPGAA GLRDKNTKAL VVTYKELAEK GPLAEETAFD FPTTPDPVVG GHFLYSFGAA 610 620 630 640 650 660 AVEVEVDLLT GDVKLIDCEH AIAAGPVVSP QGYRGQIEGG AAMALGYTLM EEAKMTDGRY 670 680 690 700 710 720 AAENLDHYLI PGIKDVPDMK LIAIEDLMKG DVYGPRGVGE IGTIAITPAI VKAVHDAVGC 730 740 WINKLPISRE ELLEAIDRKG LKQWT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator." Schultz A.C., Nygaard P., Saxild H.H. J. Bacteriol. 183:3293-3302(2001) [PubMed: 11344136] [Abstract] Cited for: FUNCTION. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB15238.1.
PIR	B70017.
RefSeq	NP_391128.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936686.
GenomeReviews	Gene locus BSU32480 in contig AL009126_GR.
KEGG	bsu:BSU32480.
NMPDR	fig|224308.1.peg.3254.
Organism-specific databases
SubtiList	BG13989. pucD. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O32144.
OMA	O32144. RFQLARI.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3245-MON.
BRENDA	1.17.1.4. 150.
Family and domain databases
InterPro	IPR000674. Ald_Oxase/Xan_DH_a/b. IPR008274. AldOxase/xan_DH_Mopterin-bd. IPR017609. Xanthine_dehydrogenase_dsu. [Graphical view]
Gene3D	G3DSA:3.30.365.10. Ald_xan_DH_mo_bd. 2 hits. G3DSA:3.90.1170.50. Aldxan_DH_hamm. 1 hit.
Pfam	PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. [Graphical view]
TIGRFAMs	TIGR03196. pucD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

XDHD_BACSU

Accession

Primary (citable) accession number: O32144

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)