Reviewed,
UniProtKB/Swiss-Prot O32143 (XDHE_BACSU)
Last modified
June 16, 2009.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (3) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Probable xanthine dehydrogenase subunit E Short name=XDHase subunit E EC=1.17.1.4 | ||||||
| Gene names |
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| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 173 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Oxidizes hypoxanthine and xanthine to uric acid. Ref.2 |
| Catalytic activity | Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH. |
| Cofactor | Binds 2 2Fe-2S clusters By similarity. |
| Pathway | Purine metabolism; hypoxanthine degradation; uric acid from hypoxanthine: step 1/2. Purine metabolism; hypoxanthine degradation; uric acid from hypoxanthine: step 2/2. |
| Subunit structure | Could be composed of four subunits: pucA, pucC, pucD and pucE. |
| Induction | Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions. |
| Sequence similarities | Contains 1 2Fe-2S ferredoxin-type domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine metabolism |
| Ligand | 2Fe-2S Iron Iron-sulfur Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activityInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW xanthine dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 173 | 173 | Probable xanthine dehydrogenase subunit E | PRO_0000166101 | |||||
Regions | |||||||||
| Domain | 14 – 90 | 77 | 2Fe-2S ferredoxin-type | ||||||
Sites | |||||||||
| Metal binding | 52 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
| Metal binding | 57 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
| Metal binding | 60 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
| Metal binding | 72 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
| Metal binding | 110 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
| Metal binding | 113 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
| Metal binding | 145 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
| Metal binding | 147 | 1 | Iron-sulfur (2Fe-2S) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [2] | "Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator." Schultz A.C., Nygaard P., Saxild H.H. J. Bacteriol. 183:3293-3302(2001) [PubMed: 11344136] [Abstract] Cited for: FUNCTION. Strain: 168. |
Cross-references
Sequence databases | |
|---|---|
| AL009126 Genomic DNA. Translation: CAB15237.1. | |
| PIR | A70017. |
| RefSeq | NP_391127.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HLR based on UniProtKB Q46509. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 936680. |
| GenomeReviews | Gene locus BSU32470 in contig AL009126_GR. |
| KEGG | bsu:BSU32470. |
| NMPDR | fig|224308.1.peg.3253. |
Organism-specific databases | |
| SubtiList | BG13988. pucE. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | O32143. |
| OMA | O32143. MAVWANG. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU3244-MON. |
| BRENDA | 1.17.1.4. 150. |
Family and domain databases | |
| InterPro | IPR002888. 2Fe-2S_bd. IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR017611. Xanthine_dehydrogenase_esu. [Graphical view] |
| Pfam | PF00111. Fer2. 1 hit. PF01799. Fer2_2. 1 hit. [Graphical view] |
| ProDom | PD186071. 2Fe-2S_bind. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR03198. pucE. 1 hit. |
| PROSITE | PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | XDHE_BACSU | ||||||||
| Accession | Primary (citable) accession number: O32143 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
