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Protein

Probable xanthine dehydrogenase subunit E

Gene

pucE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Oxidizes hypoxanthine and xanthine to uric acid.1 Publication

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity

Pathwayi: hypoxanthine degradation

This protein is involved in step 1 and 2 of the subpathway that synthesizes urate from hypoxanthine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable xanthine dehydrogenase subunit C (pucC), Probable xanthine dehydrogenase subunit A (pucA), Probable xanthine dehydrogenase subunit D (pucD), Probable xanthine dehydrogenase subunit E (pucE)
  2. Probable xanthine dehydrogenase subunit C (pucC), Probable xanthine dehydrogenase subunit A (pucA), Probable xanthine dehydrogenase subunit D (pucD), Probable xanthine dehydrogenase subunit E (pucE)
This subpathway is part of the pathway hypoxanthine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes urate from hypoxanthine, the pathway hypoxanthine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi57Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi60Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi72Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi110Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1
Metal bindingi113Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1
Metal bindingi145Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1
Metal bindingi147Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU32470-MONOMER
UniPathwayiUPA00604; UER00661
UPA00604; UER00662

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xanthine dehydrogenase subunit E (EC:1.17.1.4)
Short name:
XDHase subunit E
Gene namesi
Name:pucE
Synonyms:yurB
Ordered Locus Names:BSU32470
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001661011 – 173Probable xanthine dehydrogenase subunit EAdd BLAST173

Proteomic databases

PaxDbiO32143
PRIDEiO32143

Expressioni

Inductioni

Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.

Interactioni

Subunit structurei

Could be composed of four subunits: PucA, PucC, PucD and PucE.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017616

Structurei

3D structure databases

ProteinModelPortaliO32143
SMRiO32143
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 902Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST77

Phylogenomic databases

eggNOGiENOG4107YV9 Bacteria
COG2080 LUCA
HOGENOMiHOG000166647
InParanoidiO32143
KOiK00087
OMAiENLHPMQ
PhylomeDBiO32143

Family and domain databases

Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR002888 2Fe-2S-bd
IPR036884 2Fe-2S-bd_dom_sf
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR017611 Xanthine_dehydrogenase_esu
PfamiView protein in Pfam
PF00111 Fer2, 1 hit
PF01799 Fer2_2, 1 hit
SUPFAMiSSF47741 SSF47741, 1 hit
SSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR03198 pucE, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit

Sequencei

Sequence statusi: Complete.

O32143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIKEAGPFP VKKEQFRMTV NGQAWEVAAV PTTHLSDLLR KEFQLTGTKV
60 70 80 90 100
SCGIGRCGAC SILIDGKLAN ACMTMAYQAD GHSITTIEGL QKEELDMCQT
110 120 130 140 150
AFLEEGGFQC GYCTPGMIIA LKALFRETPQ PSDKDIEEGL AGNLCRCTGY
160 170
GGIMRSACRI RRELNGGRRE SGF
Length:173
Mass (Da):18,838
Last modified:January 1, 1998 - v1
Checksum:i48E7D4CBB76BB321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15237.1
PIRiA70017
RefSeqiNP_391127.1, NC_000964.3
WP_003243276.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15237; CAB15237; BSU32470
GeneIDi936680
KEGGibsu:BSU32470
PATRICifig|224308.179.peg.3516

Similar proteinsi

Entry informationi

Entry nameiXDHE_BACSU
AccessioniPrimary (citable) accession number: O32143
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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