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Protein

Probable xanthine dehydrogenase subunit E

Gene

pucE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Oxidizes hypoxanthine and xanthine to uric acid.1 Publication

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity

Pathwayi: hypoxanthine degradation

This protein is involved in step 1 and 2 of the subpathway that synthesizes urate from hypoxanthine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable xanthine dehydrogenase subunit C (pucC), Probable xanthine dehydrogenase subunit A (pucA), Probable xanthine dehydrogenase subunit D (pucD), Probable xanthine dehydrogenase subunit E (pucE)
  2. Probable xanthine dehydrogenase subunit C (pucC), Probable xanthine dehydrogenase subunit A (pucA), Probable xanthine dehydrogenase subunit D (pucD), Probable xanthine dehydrogenase subunit E (pucE)
This subpathway is part of the pathway hypoxanthine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes urate from hypoxanthine, the pathway hypoxanthine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi57Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi60Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi72Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation1
Metal bindingi110Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1
Metal bindingi113Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1
Metal bindingi145Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1
Metal bindingi147Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPurine metabolism
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU32470-MONOMER.
UniPathwayiUPA00604; UER00661.
UPA00604; UER00662.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xanthine dehydrogenase subunit E (EC:1.17.1.4)
Short name:
XDHase subunit E
Gene namesi
Name:pucE
Synonyms:yurB
Ordered Locus Names:BSU32470
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001661011 – 173Probable xanthine dehydrogenase subunit EAdd BLAST173

Proteomic databases

PaxDbiO32143.

Expressioni

Inductioni

Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.

Interactioni

Subunit structurei

Could be composed of four subunits: PucA, PucC, PucD and PucE.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017616.

Structurei

3D structure databases

ProteinModelPortaliO32143.
SMRiO32143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 902Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST77

Phylogenomic databases

eggNOGiENOG4107YV9. Bacteria.
COG2080. LUCA.
HOGENOMiHOG000166647.
InParanoidiO32143.
KOiK00087.
OMAiCTPGMIT.
PhylomeDBiO32143.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
InterProiView protein in InterPro
IPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR017611. Xanthine_dehydrogenase_esu.
PfamiView protein in Pfam
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR03198. pucE. 1 hit.
PROSITEiView protein in PROSITE
PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O32143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIKEAGPFP VKKEQFRMTV NGQAWEVAAV PTTHLSDLLR KEFQLTGTKV
60 70 80 90 100
SCGIGRCGAC SILIDGKLAN ACMTMAYQAD GHSITTIEGL QKEELDMCQT
110 120 130 140 150
AFLEEGGFQC GYCTPGMIIA LKALFRETPQ PSDKDIEEGL AGNLCRCTGY
160 170
GGIMRSACRI RRELNGGRRE SGF
Length:173
Mass (Da):18,838
Last modified:January 1, 1998 - v1
Checksum:i48E7D4CBB76BB321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15237.1.
PIRiA70017.
RefSeqiNP_391127.1. NC_000964.3.
WP_003243276.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15237; CAB15237; BSU32470.
GeneIDi936680.
KEGGibsu:BSU32470.
PATRICi18978468. VBIBacSub10457_3397.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15237.1.
PIRiA70017.
RefSeqiNP_391127.1. NC_000964.3.
WP_003243276.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliO32143.
SMRiO32143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017616.

Proteomic databases

PaxDbiO32143.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15237; CAB15237; BSU32470.
GeneIDi936680.
KEGGibsu:BSU32470.
PATRICi18978468. VBIBacSub10457_3397.

Phylogenomic databases

eggNOGiENOG4107YV9. Bacteria.
COG2080. LUCA.
HOGENOMiHOG000166647.
InParanoidiO32143.
KOiK00087.
OMAiCTPGMIT.
PhylomeDBiO32143.

Enzyme and pathway databases

UniPathwayiUPA00604; UER00661.
UPA00604; UER00662.
BioCyciBSUB:BSU32470-MONOMER.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
InterProiView protein in InterPro
IPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR012675. Beta-grasp_dom.
IPR017611. Xanthine_dehydrogenase_esu.
PfamiView protein in Pfam
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR03198. pucE. 1 hit.
PROSITEiView protein in PROSITE
PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiXDHE_BACSU
AccessioniPrimary (citable) accession number: O32143
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: January 1, 1998
Last modified: February 15, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.