ID   PUCR_BACSU              Reviewed;         531 AA.
AC   O32138;
DT   19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   27-MAR-2024, entry version 121.
DE   RecName: Full=Purine catabolism regulatory protein;
GN   Name=pucR; Synonyms=yunI; OrderedLocusNames=BSU32420;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   FUNCTION.
RC   STRAIN=168;
RX   PubMed=11344136; DOI=10.1128/jb.183.11.3293-3302.2001;
RA   Schultz A.C., Nygaard P., Saxild H.H.;
RT   "Functional analysis of 14 genes that constitute the purine catabolic
RT   pathway in Bacillus subtilis and evidence for a novel regulon controlled by
RT   the PucR transcription activator.";
RL   J. Bacteriol. 183:3293-3302(2001).
RN   [3]
RP   FUNCTION.
RC   STRAIN=168;
RX   PubMed=12029039; DOI=10.1128/jb.184.12.3232-3241.2002;
RA   Beier L., Nygaard P., Jarmer H., Saxild H.H.;
RT   "Transcription analysis of the Bacillus subtilis PucR regulon and
RT   identification of a cis-acting sequence required for PucR-regulated
RT   expression of genes involved in purine catabolism.";
RL   J. Bacteriol. 184:3232-3241(2002).
CC   -!- FUNCTION: Activates the expression of pucFG, pucH, pucI, pucJKLM and
CC       guaD, while it represses pucABCDE and its own expression.
CC       {ECO:0000269|PubMed:11344136, ECO:0000269|PubMed:12029039}.
CC   -!- INDUCTION: Expression is very low in excess nitrogen (glutamate plus
CC       ammonia) and is induced during limiting-nitrogen conditions
CC       (glutamate). Expression slightly decreases when allantoin is added
CC       during limiting-nitrogen conditions.
CC   -!- SIMILARITY: Belongs to the CdaR family. {ECO:0000305}.
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DR   EMBL; AL009126; CAB15232.1; -; Genomic_DNA.
DR   PIR; D70016; D70016.
DR   RefSeq; NP_391122.1; NC_000964.3.
DR   RefSeq; WP_003244351.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O32138; -.
DR   SMR; O32138; -.
DR   STRING; 224308.BSU32420; -.
DR   PaxDb; 224308-BSU32420; -.
DR   EnsemblBacteria; CAB15232; CAB15232; BSU_32420.
DR   GeneID; 937221; -.
DR   KEGG; bsu:BSU32420; -.
DR   PATRIC; fig|224308.179.peg.3509; -.
DR   eggNOG; COG2508; Bacteria.
DR   InParanoid; O32138; -.
DR   OrthoDB; 142218at2; -.
DR   PhylomeDB; O32138; -.
DR   BioCyc; BSUB:BSU32420-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.2840; PucR C-terminal helix-turn-helix domain; 1.
DR   InterPro; IPR041522; CdaR_GGDEF.
DR   InterPro; IPR025736; PucR_C-HTH_dom.
DR   InterPro; IPR042070; PucR_C-HTH_sf.
DR   InterPro; IPR012914; PucR_dom.
DR   PANTHER; PTHR33744; CARBOHYDRATE DIACID REGULATOR; 1.
DR   PANTHER; PTHR33744:SF15; CARBOHYDRATE DIACID REGULATOR; 1.
DR   Pfam; PF17853; GGDEF_2; 1.
DR   Pfam; PF13556; HTH_30; 1.
DR   Pfam; PF07905; PucR; 1.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Purine metabolism; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..531
FT                   /note="Purine catabolism regulatory protein"
FT                   /id="PRO_0000165945"
SQ   SEQUENCE   531 AA;  60514 MW;  CC2DA7F96247452F CRC64;
     MNILDVMKIP AFENANLIAG KAGGEREVQH VNMMDAPDIV DFLHKNELLV TTAYHLKDHP
     HQLSELIRQM AKRGCAGLGI KTKRYLEDIP KEIIELADSY AFPIIELPEH IRLGDIVNAT
     LSHILDMRSN ELQQAIYAHK KFTNHIMSGK GLQSLLKKVS DILQLPVLLL DQHAKMLSAS
     HQISVETEKL KGTLNTVSGP FFTCFSTISD QKTYSVLPIY NHEKNCGYLL IPDMVQAGDK
     GLILTIEQAA NVISFELLKE NALKQFSRRA RNEFFNNFIE RTFSSDDEIK NRAKEFKLRW
     DQKYMCIAGK LDRNDESISF TENQLASDSV FEFLEGELSA FPFPPHFFMK GNVGIILIEA
     TDSWSEMHAS VISFLEQFQT QVSAQFKRTV SFGISNICQK LIDVPDAFTE ASDALQSGHL
     SRSTAFIQVY HAKDVPELLR LLPVEDLKKF YNSTLQSLAE KQQEDQSLLH TLSVYLETHC
     QISETAKRLY VHRNTVIYRL EKCEELLGKS LKDPETTMRL RLALRMQRLI S
//