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O32138 (PUCR_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purine catabolism regulatory protein
Gene names
Name:pucR
Synonyms:yunI
Ordered Locus Names:BSU32420
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activates the expression of pucFG, pucH, pucI, pucJKLM and guaD, while it represses pucABCDE and its own expression. Ref.2 Ref.3

Induction

Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression slightly decreases when allantoin is added during limiting-nitrogen conditions.

Sequence similarities

Belongs to the CdaR family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 531531Purine catabolism regulatory protein
PRO_0000165945

Sequences

Sequence LengthMass (Da)Tools
O32138 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: CC2DA7F96247452F

FASTA53160,514
        10         20         30         40         50         60 
MNILDVMKIP AFENANLIAG KAGGEREVQH VNMMDAPDIV DFLHKNELLV TTAYHLKDHP 

        70         80         90        100        110        120 
HQLSELIRQM AKRGCAGLGI KTKRYLEDIP KEIIELADSY AFPIIELPEH IRLGDIVNAT 

       130        140        150        160        170        180 
LSHILDMRSN ELQQAIYAHK KFTNHIMSGK GLQSLLKKVS DILQLPVLLL DQHAKMLSAS 

       190        200        210        220        230        240 
HQISVETEKL KGTLNTVSGP FFTCFSTISD QKTYSVLPIY NHEKNCGYLL IPDMVQAGDK 

       250        260        270        280        290        300 
GLILTIEQAA NVISFELLKE NALKQFSRRA RNEFFNNFIE RTFSSDDEIK NRAKEFKLRW 

       310        320        330        340        350        360 
DQKYMCIAGK LDRNDESISF TENQLASDSV FEFLEGELSA FPFPPHFFMK GNVGIILIEA 

       370        380        390        400        410        420 
TDSWSEMHAS VISFLEQFQT QVSAQFKRTV SFGISNICQK LIDVPDAFTE ASDALQSGHL 

       430        440        450        460        470        480 
SRSTAFIQVY HAKDVPELLR LLPVEDLKKF YNSTLQSLAE KQQEDQSLLH TLSVYLETHC 

       490        500        510        520        530 
QISETAKRLY VHRNTVIYRL EKCEELLGKS LKDPETTMRL RLALRMQRLI S 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[2]"Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator."
Schultz A.C., Nygaard P., Saxild H.H.
J. Bacteriol. 183:3293-3302(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168.
[3]"Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism."
Beier L., Nygaard P., Jarmer H., Saxild H.H.
J. Bacteriol. 184:3232-3241(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL009126 Genomic DNA. Translation: CAB15232.1.
PIRD70016.
RefSeqNP_391122.1. NC_000964.3.

3D structure databases

ProteinModelPortalO32138.
SMRO32138. Positions 470-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU32420.

Proteomic databases

PaxDbO32138.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15232; CAB15232; BSU32420.
GeneID937221.
KEGGbsu:BSU32420.
PATRIC18978456. VBIBacSub10457_3391.

Organism-specific databases

GenoListBSU32420. [Micado]

Phylogenomic databases

eggNOGCOG2508.
HOGENOMHOG000009087.
KOK09684.
OMARNEPVYI.
OrthoDBEOG6038SW.
ProtClustDBCLSK872814.

Enzyme and pathway databases

BioCycBSUB:BSU32420-MONOMER.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR009057. Homeodomain-like.
IPR025736. PucR_C-HTH_dom.
IPR012914. PucR_dom.
[Graphical view]
PfamPF13556. HTH_30. 1 hit.
PF07905. PucR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUCR_BACSU
AccessionPrimary (citable) accession number: O32138
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList