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Protein

Purine catabolism regulatory protein

Gene

pucR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Activates the expression of pucFG, pucH, pucI, pucJKLM and guaD, while it represses pucABCDE and its own expression.2 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. purine nucleobase metabolic process Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Purine metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU32420-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine catabolism regulatory protein
Gene namesi
Name:pucR
Synonyms:yunI
Ordered Locus Names:BSU32420
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU32420. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Purine catabolism regulatory proteinPRO_0000165945Add
BLAST

Proteomic databases

PaxDbiO32138.

Expressioni

Inductioni

Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression slightly decreases when allantoin is added during limiting-nitrogen conditions.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU32420.

Structurei

3D structure databases

ProteinModelPortaliO32138.
SMRiO32138. Positions 470-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CdaR family.Curated

Phylogenomic databases

eggNOGiCOG2508.
HOGENOMiHOG000009087.
InParanoidiO32138.
KOiK09684.
OMAiYLETHCQ.
OrthoDBiEOG6038SW.
PhylomeDBiO32138.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR025736. PucR_C-HTH_dom.
IPR012914. PucR_dom.
[Graphical view]
PfamiPF13556. HTH_30. 1 hit.
PF07905. PucR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32138-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNILDVMKIP AFENANLIAG KAGGEREVQH VNMMDAPDIV DFLHKNELLV
60 70 80 90 100
TTAYHLKDHP HQLSELIRQM AKRGCAGLGI KTKRYLEDIP KEIIELADSY
110 120 130 140 150
AFPIIELPEH IRLGDIVNAT LSHILDMRSN ELQQAIYAHK KFTNHIMSGK
160 170 180 190 200
GLQSLLKKVS DILQLPVLLL DQHAKMLSAS HQISVETEKL KGTLNTVSGP
210 220 230 240 250
FFTCFSTISD QKTYSVLPIY NHEKNCGYLL IPDMVQAGDK GLILTIEQAA
260 270 280 290 300
NVISFELLKE NALKQFSRRA RNEFFNNFIE RTFSSDDEIK NRAKEFKLRW
310 320 330 340 350
DQKYMCIAGK LDRNDESISF TENQLASDSV FEFLEGELSA FPFPPHFFMK
360 370 380 390 400
GNVGIILIEA TDSWSEMHAS VISFLEQFQT QVSAQFKRTV SFGISNICQK
410 420 430 440 450
LIDVPDAFTE ASDALQSGHL SRSTAFIQVY HAKDVPELLR LLPVEDLKKF
460 470 480 490 500
YNSTLQSLAE KQQEDQSLLH TLSVYLETHC QISETAKRLY VHRNTVIYRL
510 520 530
EKCEELLGKS LKDPETTMRL RLALRMQRLI S
Length:531
Mass (Da):60,514
Last modified:January 1, 1998 - v1
Checksum:iCC2DA7F96247452F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15232.1.
PIRiD70016.
RefSeqiNP_391122.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15232; CAB15232; BSU32420.
GeneIDi937221.
KEGGibsu:BSU32420.
PATRICi18978456. VBIBacSub10457_3391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15232.1.
PIRiD70016.
RefSeqiNP_391122.1. NC_000964.3.

3D structure databases

ProteinModelPortaliO32138.
SMRiO32138. Positions 470-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU32420.

Proteomic databases

PaxDbiO32138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15232; CAB15232; BSU32420.
GeneIDi937221.
KEGGibsu:BSU32420.
PATRICi18978456. VBIBacSub10457_3391.

Organism-specific databases

GenoListiBSU32420. [Micado]

Phylogenomic databases

eggNOGiCOG2508.
HOGENOMiHOG000009087.
InParanoidiO32138.
KOiK09684.
OMAiYLETHCQ.
OrthoDBiEOG6038SW.
PhylomeDBiO32138.

Enzyme and pathway databases

BioCyciBSUB:BSU32420-MONOMER.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR025736. PucR_C-HTH_dom.
IPR012914. PucR_dom.
[Graphical view]
PfamiPF13556. HTH_30. 1 hit.
PF07905. PucR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  2. "Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator."
    Schultz A.C., Nygaard P., Saxild H.H.
    J. Bacteriol. 183:3293-3302(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 168.
  3. "Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism."
    Beier L., Nygaard P., Jarmer H., Saxild H.H.
    J. Bacteriol. 184:3232-3241(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 168.

Entry informationi

Entry nameiPUCR_BACSU
AccessioniPrimary (citable) accession number: O32138
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.