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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi261 – 2611Manganese 2By similarity
Metal bindingi266 – 2661Manganese 1By similarity
Metal bindingi266 – 2661Manganese 2By similarity
Active sitei273 – 2731Sequence analysis
Metal bindingi284 – 2841Manganese 2By similarity
Metal bindingi343 – 3431Manganese 1By similarity
Metal bindingi345 – 3451Manganese 1By similarity
Metal bindingi345 – 3451Manganese 2By similarity
Active sitei347 – 3471Sequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU32050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Synonyms:yuiE
Ordered Locus Names:BSU32050
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Probable cytosol aminopeptidasePRO_0000165722Add
BLAST

Proteomic databases

PaxDbiO32106.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017401.

Structurei

3D structure databases

ProteinModelPortaliO32106.
SMRiO32106. Positions 1-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
InParanoidiO32106.
KOiK01255.
OMAiGIVGMKS.
PhylomeDBiO32106.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O32106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYAVQQSEH TETLVVGLFQ KSQLTGKALE IDEMLEGHLT QLLKEGDVSA
60 70 80 90 100
KPNQVSKVFP PSSAGMKRIY FVGLGREANY SFEQAKERFA HVFQAIHKDR
110 120 130 140 150
KQETAVLLDT FISEDVPPAD AAHALAESCL LASYEVQDYK HKSNEPDKQI
160 170 180 190 200
EAVYVVTDED TQEVQAGLRV GQAYGQGTNS ARTLVNMPGN MLTATDLASY
210 220 230 240 250
AEELAAKYDF ECEILEKSEM EELGMGGILA VNQGSTEPPK MIVLKYQGKK
260 270 280 290 300
EWEDVVGLVG KGITFDTGGY SIKTKSGIVG MKSDMGGAAA VLGAMETIGE
310 320 330 340 350
LRPEQNVLCV IPSTDNMISG GAMKPDDVIV SLSGKTIEIL NTDAEGRLVL
360 370 380 390 400
ADGITYAKQH GASVLVDVAT LTGGVVVALG TETTGAMTND QSFYQQVADA
410 420 430 440 450
AQECGEAIWQ LPITEKDKKR VKSSQMADLS NSPGREGHAI MAGTFLGEFA
460 470 480 490 500
ESTPWVHLDI AGTATANKAT CFGPAGATGV MARTLATLAE RFTLEEDKNE
Length:500
Mass (Da):53,658
Last modified:January 1, 1998 - v1
Checksum:i3E82968F66566559
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15195.1.
PIRiF70012.
RefSeqiNP_391085.1. NC_000964.3.
WP_003228733.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15195; CAB15195; BSU32050.
GeneIDi936587.
KEGGibsu:BSU32050.
PATRICi18978382. VBIBacSub10457_3354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15195.1.
PIRiF70012.
RefSeqiNP_391085.1. NC_000964.3.
WP_003228733.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliO32106.
SMRiO32106. Positions 1-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017401.

Proteomic databases

PaxDbiO32106.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15195; CAB15195; BSU32050.
GeneIDi936587.
KEGGibsu:BSU32050.
PATRICi18978382. VBIBacSub10457_3354.

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
InParanoidiO32106.
KOiK01255.
OMAiGIVGMKS.
PhylomeDBiO32106.

Enzyme and pathway databases

BioCyciBSUB:BSU32050-MONOMER.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_BACSU
AccessioniPrimary (citable) accession number: O32106
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.