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Protein

Cysteine dioxygenase

Gene

cdoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cysteine + O2 = 3-sulfinoalanine.1 Publication

Cofactori

Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity

Kineticsi

  1. KM=3.0 mM for cysteine1 Publication

    pH dependencei

    Optimum pH is 6.2.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi75 – 751Iron; via tele nitrogen; catalytic1 Publication
    Metal bindingi77 – 771Iron; via tele nitrogen; catalytic1 Publication
    Metal bindingi125 – 1251Iron; via tele nitrogen; catalytic1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU31140-MONOMER.
    BRENDAi1.13.11.20. 658.
    SABIO-RKO32085.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine dioxygenase (EC:1.13.11.20)
    Short name:
    CDO
    Gene namesi
    Name:cdoA
    Synonyms:yubC
    Ordered Locus Names:BSU31140
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570 Componenti: Chromosome

    Organism-specific databases

    GenoListiBSU31140. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 161161Cysteine dioxygenasePRO_0000360761Add
    BLAST

    Proteomic databases

    PaxDbiO32085.

    Expressioni

    Developmental stagei

    Up-regulated upon sporulation.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100016931.

    Structurei

    Secondary structure

    1
    161
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 119Combined sources
    Helixi19 – 268Combined sources
    Helixi32 – 365Combined sources
    Helixi37 – 393Combined sources
    Beta strandi44 – 5512Combined sources
    Beta strandi60 – 667Combined sources
    Beta strandi74 – 763Combined sources
    Beta strandi81 – 9818Combined sources
    Beta strandi100 – 11213Combined sources
    Beta strandi116 – 1194Combined sources
    Beta strandi124 – 1285Combined sources
    Beta strandi131 – 1333Combined sources
    Beta strandi135 – 1439Combined sources
    Beta strandi149 – 1513Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3EQEX-ray2.82A/B1-161[»]
    4QM8X-ray2.82A/B1-161[»]
    4QM9X-ray2.30A/B1-161[»]
    ProteinModelPortaliO32085.
    SMRiO32085. Positions 1-154.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO32085.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cysteine dioxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG236067.
    HOGENOMiHOG000080196.
    InParanoidiO32085.
    KOiK00456.
    OMAiCILIVNG.
    OrthoDBiEOG67X1TS.
    PhylomeDBiO32085.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR010300. Cys_dOase_I.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PfamiPF05995. CDO_I. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O32085-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MELYECIQDI FGGLKNPSVK DLATSLKQIP NAAKLSQPYI KEPDQYAYGR
    60 70 80 90 100
    NAIYRNNELE IIVINIPPNK ETTVHDHGQS IGCAMVLEGK LLNSIYRSTG
    110 120 130 140 150
    EHAELSNSYF VHEGECLIST KGLIHKMSNP TSERMVSLHV YSPPLEDMTV
    160
    FEEQKEVLEN S
    Length:161
    Mass (Da):18,127
    Last modified:January 1, 1998 - v1
    Checksum:i9580D43AE8AFE016
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL009126 Genomic DNA. Translation: CAB15092.1.
    PIRiF70006.
    RefSeqiNP_390992.1. NC_000964.3.
    WP_003243534.1. NZ_JNCM01000033.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15092; CAB15092; BSU31140.
    GeneIDi938837.
    KEGGibsu:BSU31140.
    PATRICi18978182. VBIBacSub10457_3255.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL009126 Genomic DNA. Translation: CAB15092.1.
    PIRiF70006.
    RefSeqiNP_390992.1. NC_000964.3.
    WP_003243534.1. NZ_JNCM01000033.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3EQEX-ray2.82A/B1-161[»]
    4QM8X-ray2.82A/B1-161[»]
    4QM9X-ray2.30A/B1-161[»]
    ProteinModelPortaliO32085.
    SMRiO32085. Positions 1-154.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100016931.

    Proteomic databases

    PaxDbiO32085.

    Protocols and materials databases

    DNASUi938837.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB15092; CAB15092; BSU31140.
    GeneIDi938837.
    KEGGibsu:BSU31140.
    PATRICi18978182. VBIBacSub10457_3255.

    Organism-specific databases

    GenoListiBSU31140. [Micado]

    Phylogenomic databases

    eggNOGiNOG236067.
    HOGENOMiHOG000080196.
    InParanoidiO32085.
    KOiK00456.
    OMAiCILIVNG.
    OrthoDBiEOG67X1TS.
    PhylomeDBiO32085.

    Enzyme and pathway databases

    BioCyciBSUB:BSU31140-MONOMER.
    BRENDAi1.13.11.20. 658.
    SABIO-RKO32085.

    Miscellaneous databases

    EvolutionaryTraceiO32085.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR010300. Cys_dOase_I.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PfamiPF05995. CDO_I. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    2. "Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria."
      Dominy J.E. Jr., Simmons C.R., Karplus P.A., Gehring A.M., Stipanuk M.H.
      J. Bacteriol. 188:5561-5569(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.
    3. "Structures of Arg- and Gln-type bacterial cysteine dioxygenase homologs."
      Driggers C.M., Hartman S.J., Karplus P.A.
      Protein Sci. 24:154-161(2015) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH IRON.

    Entry informationi

    Entry nameiCDOA_BACSU
    AccessioniPrimary (citable) accession number: O32085
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: January 1, 1998
    Last modified: July 22, 2015
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although there are Cys and Trp residues in equivalent positions with the residues forming a thioether cross-link in the eukaryotic homologous sequences, no thioether cross-link is formed in this protein.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.