Cysteine dioxygenase - Bacillus subtilis

Contribute

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O32085 (CDOA_BACSU)

Last modified November 3, 2009. Version 49. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Cysteine dioxygenase
      Short name=CDO
    EC=1.13.11.20

Gene names

Name:	cdoA
Synonyms:	yubC
Ordered Locus Names:	BSU31140

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-cysteine + O₂ = 3-sulfinoalanine. Ref.2
Cofactor	Binds 1 iron ion per subunit By similarity.
Developmental stage	Up-regulated upon sporulation. Ref.2
Sequence similarities	Belongs to the cysteine dioxygenase family.
Biophysicochemical properties	Kinetic parameters: K_M=3.0 mM for cysteine pH dependence: Optimum pH is 6.2.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	L-cysteine metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

161

Cysteine dioxygenase

PRO_0000360761

Sites

Metal binding

1

Iron; catalytic By similarity

Metal binding

1

Iron; catalytic By similarity

Metal binding

1

Iron; catalytic By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

161

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

O32085-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 9580D43AE8AFE016
Show »

161

18,127

        10         20         30         40         50         60 
MELYECIQDI FGGLKNPSVK DLATSLKQIP NAAKLSQPYI KEPDQYAYGR NAIYRNNELE 

        70         80         90        100        110        120 
IIVINIPPNK ETTVHDHGQS IGCAMVLEGK LLNSIYRSTG EHAELSNSYF VHEGECLIST 

       130        140        150        160 
KGLIHKMSNP TSERMVSLHV YSPPLEDMTV FEEQKEVLEN S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria." Dominy J.E. Jr., Simmons C.R., Karplus P.A., Gehring A.M., Stipanuk M.H. J. Bacteriol. 188:5561-5569(2006) [PubMed: 16855246] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

AL009126 Genomic DNA. Translation: CAB15092.1.

PIR

RefSeq

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EQE	X-ray	2.82	A/B	1-161	[»]

ModBase

Genome annotation databases

GeneID

GenomeReviews

Gene locus BSU31140 in contig AL009126_GR.

KEGG

NMPDR

fig|224308.1.peg.3117.

Organism-specific databases

SubtiList

BG13952. yubC. [Micado]

CMR

Phylogenomic databases

HOGENOM

OMA

Enzyme and pathway databases

BioCyc

BSUB224308:BSU3109-MON.

BRENDA

1.13.11.20. 150.

Family and domain databases

InterPro

IPR010300. Cys_dOase_I.
[Graphical view]

Pfam

PF05995. CDO_I. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

CDOA_BACSU

Accession

Primary (citable) accession number: O32085

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents