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Protein

Cysteine dioxygenase

Gene

cdoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cysteine + O2 = 3-sulfinoalanine.1 Publication

Cofactori

Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity

Kineticsi

  1. KM=3.0 mM for cysteine1 Publication

pH dependencei

Optimum pH is 6.2.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751Iron; catalyticBy similarity
Metal bindingi77 – 771Iron; catalyticBy similarity
Metal bindingi125 – 1251Iron; catalyticBy similarity

GO - Molecular functioni

  1. cysteine dioxygenase activity Source: UniProtKB-EC
  2. iron ion binding Source: InterPro

GO - Biological processi

  1. L-cysteine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU31140-MONOMER.
BRENDAi1.13.11.20. 700.
SABIO-RKO32085.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine dioxygenase (EC:1.13.11.20)
Short name:
CDO
Gene namesi
Name:cdoA
Synonyms:yubC
Ordered Locus Names:BSU31140
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU31140. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Cysteine dioxygenasePRO_0000360761Add
BLAST

Proteomic databases

PaxDbiO32085.

Expressioni

Developmental stagei

Up-regulated upon sporulation.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU31140.

Structurei

Secondary structure

1
161
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 119Combined sources
Helixi19 – 268Combined sources
Helixi32 – 365Combined sources
Helixi37 – 393Combined sources
Beta strandi44 – 5512Combined sources
Beta strandi60 – 667Combined sources
Beta strandi74 – 763Combined sources
Beta strandi81 – 9818Combined sources
Beta strandi100 – 11213Combined sources
Beta strandi116 – 1194Combined sources
Beta strandi124 – 1285Combined sources
Beta strandi131 – 1333Combined sources
Beta strandi135 – 1439Combined sources
Beta strandi149 – 1513Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EQEX-ray2.82A/B1-161[»]
4QM8X-ray2.82A/B1-161[»]
4QM9X-ray2.30A/B1-161[»]
ProteinModelPortaliO32085.
SMRiO32085. Positions 1-154.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO32085.

Family & Domainsi

Sequence similaritiesi

Belongs to the cysteine dioxygenase family.Curated

Phylogenomic databases

eggNOGiNOG236067.
HOGENOMiHOG000080196.
InParanoidiO32085.
OMAiINIPPNK.
OrthoDBiEOG67X1TS.
PhylomeDBiO32085.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

O32085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELYECIQDI FGGLKNPSVK DLATSLKQIP NAAKLSQPYI KEPDQYAYGR
60 70 80 90 100
NAIYRNNELE IIVINIPPNK ETTVHDHGQS IGCAMVLEGK LLNSIYRSTG
110 120 130 140 150
EHAELSNSYF VHEGECLIST KGLIHKMSNP TSERMVSLHV YSPPLEDMTV
160
FEEQKEVLEN S
Length:161
Mass (Da):18,127
Last modified:January 1, 1998 - v1
Checksum:i9580D43AE8AFE016
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15092.1.
PIRiF70006.
RefSeqiNP_390992.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15092; CAB15092; BSU31140.
GeneIDi938837.
KEGGibsu:BSU31140.
PATRICi18978182. VBIBacSub10457_3255.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB15092.1.
PIRiF70006.
RefSeqiNP_390992.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EQEX-ray2.82A/B1-161[»]
4QM8X-ray2.82A/B1-161[»]
4QM9X-ray2.30A/B1-161[»]
ProteinModelPortaliO32085.
SMRiO32085. Positions 1-154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU31140.

Proteomic databases

PaxDbiO32085.

Protocols and materials databases

DNASUi938837.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15092; CAB15092; BSU31140.
GeneIDi938837.
KEGGibsu:BSU31140.
PATRICi18978182. VBIBacSub10457_3255.

Organism-specific databases

GenoListiBSU31140. [Micado]

Phylogenomic databases

eggNOGiNOG236067.
HOGENOMiHOG000080196.
InParanoidiO32085.
OMAiINIPPNK.
OrthoDBiEOG67X1TS.
PhylomeDBiO32085.

Enzyme and pathway databases

BioCyciBSUB:BSU31140-MONOMER.
BRENDAi1.13.11.20. 700.
SABIO-RKO32085.

Miscellaneous databases

EvolutionaryTraceiO32085.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  2. "Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria."
    Dominy J.E. Jr., Simmons C.R., Karplus P.A., Gehring A.M., Stipanuk M.H.
    J. Bacteriol. 188:5561-5569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCDOA_BACSU
AccessioniPrimary (citable) accession number: O32085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: March 4, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.