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O32085 (CDOA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine dioxygenase
Short name=CDO
EC=1.13.11.20
Gene names
Name:cdoA
Synonyms:yubC
Ordered Locus Names:BSU31140
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-cysteine + O2 = 3-sulfinoalanine. Ref.2

Cofactor

Binds 1 iron ion per subunit By similarity.

Developmental stage

Up-regulated upon sporulation. Ref.2

Sequence similarities

Belongs to the cysteine dioxygenase family.

Biophysicochemical properties

Kinetic parameters:

KM=3.0 mM for cysteine Ref.2

pH dependence:

Optimum pH is 6.2.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Cysteine dioxygenase
PRO_0000360761

Sites

Metal binding751Iron; catalytic By similarity
Metal binding771Iron; catalytic By similarity
Metal binding1251Iron; catalytic By similarity

Secondary structure

1 ......................... 161
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O32085 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 9580D43AE8AFE016

FASTA16118,127
        10         20         30         40         50         60 
MELYECIQDI FGGLKNPSVK DLATSLKQIP NAAKLSQPYI KEPDQYAYGR NAIYRNNELE 

        70         80         90        100        110        120 
IIVINIPPNK ETTVHDHGQS IGCAMVLEGK LLNSIYRSTG EHAELSNSYF VHEGECLIST 

       130        140        150        160 
KGLIHKMSNP TSERMVSLHV YSPPLEDMTV FEEQKEVLEN S

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[2]"Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria."
Dominy J.E. Jr., Simmons C.R., Karplus P.A., Gehring A.M., Stipanuk M.H.
J. Bacteriol. 188:5561-5569(2006) [PubMed: 16855246] [Abstract]
Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL009126 Genomic DNA. Translation: CAB15092.1.
PIRF70006.
RefSeqNP_390992.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3EQEX-ray2.82A/B1-161[»]
ProteinModelPortalO32085.
SMRO32085. Positions 1-154.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003406; EBBACP00000003406; EBBACG00000003399.
GeneID938837.
GenomeReviewsGene locus BSU31140 in contig AL009126_GR.
KEGGbsu:BSU31140.
NMPDRfig|224308.1.peg.3117.
PATRIC18978182. VBIBacSub10457_3255.

Organism-specific databases

GenoListBSU31140. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000005627.
OMATEGNIFT.
ProtClustDBCLSK219258.

Enzyme and pathway databases

BioCycBSUB:BSU31140-MONOMER.
BRENDA1.13.11.20. 700.

Family and domain databases

InterProIPR011051. Cupin_RmlC_type.
IPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
Gene3DG3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
PfamPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMSSF51182. RmlC_like_cupin. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCDOA_BACSU
AccessionPrimary (citable) accession number: O32085
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents