Reviewed, UniProtKB/Swiss-Prot O32083 (LYTG_BACSU)

Names and origin

Protein names

Recommended name:
    Exo-glucosaminidase lytG
    EC=3.2.1.-
Alternative name(s):
    Exo-beta-N-acetylglucosaminidase lytG
    Peptidoglycan hydrolase lytG
    Autolysin lytG

Gene names

Name:	lytG
Synonyms:	yubE
Ordered Locus Names:	BSU31120

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Acts processively from the ends of the glycan strands. Also plays a role in motility, chemotaxis and cell division. Ref.2
Catalytic activity	Hydrolysis of 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid residues in a peptidoglycan.
Cofactor	Magnesium. Ref.2
Enzyme regulation	Inhibited by EDTA.
Subcellular location	Secreted › cell wall. Ref.2
Developmental stage	Expressed during vegetative growth.
Induction	Expressed under control of sigma-A RNA polymerase.
Sequence similarities	Belongs to the glycosyl hydrolase 73 family.
Biophysicochemical properties	pH dependence: Optimal pH is about 5.7.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Ligand	Magnesium
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall macromolecule metabolic process Inferred from electronic annotation. Source: InterPro ciliary or flagellar motility Inferred from electronic annotation. Source: InterPro peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	bacterial-type flagellum Inferred from electronic annotation. Source: InterPro cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidase activity Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 282	253	Exo-glucosaminidase lytG		PRO_0000012119

Sequences

	Sequence		Length	Mass (Da)
	O32083-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: DEA15D5AFA73F327	FASTA	282	31,901
	10 20 30 40 50 60 MARKKLKKRK LLISLFFLVS IPLALFVLAT TLSKPIEISK ETEEIDEQQV FIDSLSGHAQ 70 80 90 100 110 120 ILYEKYHVLP SITIAQAILE SDWGNSELAA KANNLFGVKG NYKGHHVTME TDEVEKGKRK 130 140 150 160 170 180 TIRAKFRKYS TFFESMDDHA QLFVRGTSWN KKKYKPVLEA GNYKEAATAL QTSGYATDPD 190 200 210 220 230 240 YADKISAIVE KYDLDEYDEV NPSLKSVDLN ASIKDSAVQD VWSKPSTDDR SIRLTSAQSY 250 260 270 280 VGKDIKVVSK KQKGQSVWYQ FQINDKLIGW IDDSAVEIKE AT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"LytG of Bacillus subtilis is a novel peptidoglycan hydrolase: the major active glucosaminidase." Horsburgh G.J., Atrih A., Williamson M.P., Foster S.J. Biochemistry 42:257-264(2003) [PubMed: 12525152] [Abstract] Cited for: FUNCTION, CHARACTERIZATION, COFACTOR, SUBCELLULAR LOCATION, TRANSCRIPTIONAL REGULATION. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB15090.1.
PIR	H70006.
RefSeq	NP_390990.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH73. Glycoside Hydrolase Family 73.
Genome annotation databases
GeneID	937146.
GenomeReviews	Gene locus BSU31120 in contig AL009126_GR.
KEGG	bsu:BSU31120.
NMPDR	fig|224308.1.peg.3115.
Organism-specific databases
SubtiList	BG13954. lytG. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O32083.
OMA	WGNSELA.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3107-MON.
Family and domain databases
InterPro	IPR000423. Flag_FlgJ_1. IPR013338. Lyz2. IPR002901. Mano_Glyc_endo_b_GlcNAc. [Graphical view]
Pfam	PF01832. Glucosaminidase. 1 hit. [Graphical view]
PRINTS	PR01002. FLGFLGJ.
SMART	SM00047. LYZ2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYTG_BACSU

Accession

Primary (citable) accession number: O32083

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)