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Protein

S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Gene

queA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU27720-MONOMER.
BRENDAi2.4.99.17. 658.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine:tRNA ribosyltransferase-isomeraseUniRule annotation (EC:2.4.99.17UniRule annotation)
Alternative name(s):
Queuosine biosynthesis protein QueAUniRule annotation
Gene namesi
Name:queAUniRule annotation
Ordered Locus Names:BSU27720
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001653801 – 342S-adenosylmethionine:tRNA ribosyltransferase-isomeraseAdd BLAST342

Proteomic databases

PaxDbiO32054.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015146.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Beta strandi26 – 30Combined sources5
Turni33 – 35Combined sources3
Helixi45 – 49Combined sources5
Beta strandi54 – 58Combined sources5
Beta strandi66 – 76Combined sources11
Beta strandi79 – 86Combined sources8
Beta strandi91 – 96Combined sources6
Helixi99 – 101Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi113 – 122Combined sources10
Beta strandi128 – 133Combined sources6
Helixi138 – 146Combined sources9
Helixi152 – 155Combined sources4
Beta strandi158 – 160Combined sources3
Helixi186 – 195Combined sources10
Beta strandi197 – 201Combined sources5
Beta strandi203 – 206Combined sources4
Helixi207 – 211Combined sources5
Beta strandi227 – 231Combined sources5
Helixi233 – 244Combined sources12
Beta strandi249 – 252Combined sources4
Turni254 – 256Combined sources3
Helixi257 – 265Combined sources9
Turni266 – 268Combined sources3
Beta strandi274 – 278Combined sources5
Beta strandi292 – 297Combined sources6
Helixi305 – 313Combined sources9
Helixi315 – 327Combined sources13
Beta strandi332 – 336Combined sources5
Beta strandi338 – 340Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YY3X-ray2.88A/B2-342[»]
ProteinModelPortaliO32054.
SMRiO32054.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO32054.

Family & Domainsi

Sequence similaritiesi

Belongs to the QueA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2N. Bacteria.
COG0809. LUCA.
HOGENOMiHOG000004401.
InParanoidiO32054.
KOiK07568.
OMAiYGDAMFL.
PhylomeDBiO32054.

Family and domain databases

HAMAPiMF_00113. QueA. 1 hit.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.

Sequencei

Sequence statusi: Complete.

O32054-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVDLFDFEL PERLIAQVPL EQRDASRLMV LDKHTGELTD SSFKHIISFF
60 70 80 90 100
NEGDCLVLNN TRVLPARLFG TKEDTGAKVE LLLLKQETGD KWETLAKPAK
110 120 130 140 150
RVKKGTVVTF GDGRLKAICT EELEHGGRKM EFQYDGIFYE VLESLGEMPL
160 170 180 190 200
PPYIKEQLDD KERYQTVYSK EIGSAAAPTA GLHFTEEILQ QLKDKGVQIE
210 220 230 240 250
FITLHVGLGT FRPVSADEVE EHNMHAEFYQ MSEETAAALN KVRENGGRII
260 270 280 290 300
SVGTTSTRTL ETIAGEHDGQ FKASSGWTSI FIYPGYEFKA IDGMITNFHL
310 320 330 340
PKSSLIMLVS ALAGRENILR AYNHAVEEEY RFFSFGDAML II
Length:342
Mass (Da):38,512
Last modified:January 1, 1998 - v1
Checksum:iD8A28DCC9AC05DAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15896 Genomic DNA. Translation: CAB75332.1.
AL009126 Genomic DNA. Translation: CAB14732.1.
PIRiA69688.
RefSeqiNP_390650.1. NC_000964.3.
WP_003229723.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14732; CAB14732; BSU27720.
GeneIDi937975.
KEGGibsu:BSU27720.
PATRICi18977410. VBIBacSub10457_2894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15896 Genomic DNA. Translation: CAB75332.1.
AL009126 Genomic DNA. Translation: CAB14732.1.
PIRiA69688.
RefSeqiNP_390650.1. NC_000964.3.
WP_003229723.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YY3X-ray2.88A/B2-342[»]
ProteinModelPortaliO32054.
SMRiO32054.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015146.

Proteomic databases

PaxDbiO32054.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14732; CAB14732; BSU27720.
GeneIDi937975.
KEGGibsu:BSU27720.
PATRICi18977410. VBIBacSub10457_2894.

Phylogenomic databases

eggNOGiENOG4105E2N. Bacteria.
COG0809. LUCA.
HOGENOMiHOG000004401.
InParanoidiO32054.
KOiK07568.
OMAiYGDAMFL.
PhylomeDBiO32054.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciBSUB:BSU27720-MONOMER.
BRENDAi2.4.99.17. 658.

Miscellaneous databases

EvolutionaryTraceiO32054.

Family and domain databases

HAMAPiMF_00113. QueA. 1 hit.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEA_BACSU
AccessioniPrimary (citable) accession number: O32054
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.