O32053 (TGT_BACSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 83.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Queuine tRNA-ribosyltransferase EC=2.4.2.29 Alternative name(s): Guanine insertion enzyme tRNA-guanine transglycosylase | ||||
| Gene names |
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| Organism | Bacillus subtilis | ||||
| Taxonomic identifier | 1423 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 381 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168 |
| Catalytic activity | [tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168 |
| Cofactor | Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168 |
| Pathway | tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168 |
| Sequence similarities | Belongs to the queuine tRNA-ribosyltransferase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Queuosine biosynthesis tRNA processing |
| Ligand | Metal-binding Zinc |
| Molecular function | Glycosyltransferase Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 381 | 381 | Queuine tRNA-ribosyltransferase HAMAP MF_00168 | PRO_0000135452 | |||||
Sites | |||||||||
| Active site | 96 | 1 | Nucleophile By similarity | ||||||
| Metal binding | 308 | 1 | Zinc By similarity | ||||||
| Metal binding | 310 | 1 | Zinc By similarity | ||||||
| Metal binding | 313 | 1 | Zinc By similarity | ||||||
| Metal binding | 339 | 1 | Zinc By similarity | ||||||
| Binding site | 97 | 1 | Substrate By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A 17.8 kb segment in the spoVB-nadC region of the Bacillus subtilis 168 chromosome: sequencing and ruv operon identification." Tosato V., Bolotin A., Bertani I., Valentino I., Bruschi C.V. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y15896 Genomic DNA. Translation: CAB75333.1. AL009126 Genomic DNA. Translation: CAB14731.1. |
| PIR | B69722. |
| RefSeq | NP_390649.1. NC_000964.3. |
3D structure databases | |
| ProteinModelPortal | O32053. |
| SMR | O32053. Positions 6-372. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O32053. 1 interaction. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000000945; EBBACP00000000945; EBBACG00000000943. |
| GeneID | 937528. |
| GenomeReviews | Gene locus BSU27710 in contig AL009126_GR. |
| KEGG | bsu:BSU27710. |
| NMPDR | fig|224308.1.peg.2774. |
| PATRIC | 18977408. VBIBacSub10457_2893. |
Organism-specific databases | |
| GenoList | BSU27710. [Micado] |
Phylogenomic databases | |
| GeneTree | EBGT00050000001431. |
| HOGENOM | HBG629929. |
| OMA | FMPVGTV. |
| PhylomeDB | O32053. |
| ProtClustDB | PRK00112. |
Enzyme and pathway databases | |
| BioCyc | BSUB:BSU27710-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00168. Q_tRNA_Tgt. [Tree] |
| InterPro | IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view] |
| Gene3D | G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit. |
| KO | K00773. |
| PANTHER | PTHR11962. tRNA_ribo_trans. 1 hit. |
| Pfam | PF01702. TGT. 1 hit. [Graphical view] |
| SUPFAM | SSF51713. tRNA_ribo_trans. 1 hit. |
| TIGRFAMs | TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. Tgt_general. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | TGT_BACSU | ||||||||
| Accession | Primary (citable) accession number: O32053 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |