Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O32053 (TGT_BACSU)

Last modified June 16, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme
Gene names
Name: tgt
Ordered Locus Names: BSU27710
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Hide | Top

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.

Catalytic activity

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168

[tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Hide | Top

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionqueuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Queuine tRNA-ribosyltransferase HAMAP MF_00168
PRO_0000135452

Sites

Active site961Nucleophile By similarity
Metal binding3081Zinc By similarity
Metal binding3101Zinc By similarity
Metal binding3131Zinc By similarity
Metal binding3391Zinc By similarity
Binding site971Substrate By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
O32053-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 76AD1B92883C6D36

FASTA38143,711
        10         20         30         40         50         60 
MAEQPIRYEF IKECKQTGAR LGKVHTPHGS FETPVFMPVG TLATVKTMSP EELKAMDAGI 

        70         80         90        100        110        120 
ILSNTYHLWL RPGQDIVKEA GGLHKFMNWD RAILTDSGGF QVFSLSKFRN IEEEGVHFRN 

       130        140        150        160        170        180 
HLNGDKLFLS PEKAMEIQNA LGSDIMMAFD ECPPYPAEYD YMKRSVERTS RWAERCLNAH 

       190        200        210        220        230        240 
NRQDEQGLFG IVQGGEYEDL RTQSAKDLIS LDFPGYAIGG LSVGEPKDVM NRVLEFTTPL 

       250        260        270        280        290        300 
LPKDKPRYLM GVGSPDALID GAIRGVDMFD CVLPTRIARN GTVFTAEGRL NMKNAKFERD 

       310        320        330        340        350        360 
FRPIDEECDC YTCKNYTRAY IRHLIRCNET FGLRLTTYHN LHFLLHLMEQ VRQAIREDRL 

       370        380 
GDFREEFFER YGYNKPNAKS F

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"A 17.8 kb segment in the spoVB-nadC region of the Bacillus subtilis 168 chromosome: sequencing and ruv operon identification."
Tosato V., Bolotin A., Bertani I., Valentino I., Bruschi C.V.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Hide | Top

Cross-references

Sequence databases

Y15896 Genomic DNA. Translation: CAB75333.1.
AL009126 Genomic DNA. Translation: CAB14731.1.
PIRB69722.
RefSeqNP_390649.1.

3D structure databases

HSSPHSSP built from PDB template 1K4G based on UniProtKB P28720.
ModBaseSearch...

Genome annotation databases

GeneID937528.
GenomeReviewsGene locus BSU27710 in contig AL009126_GR.
KEGGbsu:BSU27710.
NMPDRfig|224308.1.peg.2774.

Organism-specific databases

SubtiListBG12686. tgt. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMO32053.
OMAO32053. MAFDQCP.

Enzyme and pathway databases

BioCycBSUB224308:BSU2767-MON.
BRENDA2.4.2.29. 150.

Family and domain databases

HAMAPMF_00168.
[Tree]
InterProIPR004803. QtRNA_ribo_trans.
IPR002616. tRNA_ribo_trans.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Hide | Top

Entry information

Entry nameTGT_BACSU
AccessionPrimary (citable) accession number: O32053
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents