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Protein

Protein translocase subunit SecDF

Gene

secDF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient translocation of secretory pre-proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane.1 Publication
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU27650-MONOMER.

Protein family/group databases

TCDBi2.A.6.4.2. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecDF
Gene namesi
Name:secDF
Ordered Locus Names:BSU27650
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Found uniformly distributed in both the mother cell and forespore following sporulation.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Cytoplasmic1 Publication
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Topological domaini27 – 254228Extracellular1 PublicationAdd
BLAST
Transmembranei255 – 27521HelicalSequence analysisAdd
BLAST
Topological domaini276 – 2783Cytoplasmic1 Publication
Transmembranei279 – 29921HelicalSequence analysisAdd
BLAST
Topological domaini300 – 3012Extracellular1 Publication
Transmembranei302 – 32221HelicalSequence analysisAdd
BLAST
Topological domaini323 – 35331Cytoplasmic1 PublicationAdd
BLAST
Transmembranei354 – 37421HelicalSequence analysisAdd
BLAST
Topological domaini375 – 3806Extracellular1 Publication
Transmembranei381 – 40121HelicalSequence analysisAdd
BLAST
Topological domaini402 – 45352Cytoplasmic1 PublicationAdd
BLAST
Transmembranei454 – 47421HelicalSequence analysisAdd
BLAST
Topological domaini475 – 56995Extracellular1 PublicationAdd
BLAST
Transmembranei570 – 59021HelicalSequence analysisAdd
BLAST
Topological domaini591 – 5933Cytoplasmic1 Publication
Transmembranei594 – 61421HelicalSequence analysisAdd
BLAST
Topological domaini615 – 6217Extracellular1 Publication
Transmembranei622 – 64221HelicalSequence analysisAdd
BLAST
Topological domaini643 – 67735Cytoplasmic1 PublicationAdd
BLAST
Transmembranei678 – 69821HelicalSequence analysisAdd
BLAST
Topological domaini699 – 6991Extracellular1 Publication
Transmembranei700 – 72021HelicalSequence analysisAdd
BLAST
Topological domaini721 – 73717Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show a cold-sensitive phenotype and a filamentous morphology.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 737737Protein translocase subunit SecDFPRO_0000384402Add
BLAST

Proteomic databases

PaxDbiO32047.

Expressioni

Inductioni

Shows maximal expression at the beginning of post-exponential growth phase and increased expression by glucose in the post-exponential growth phase when cells are cultured on rich medium. Expressed constitutively during growth in minimal medium.1 Publication

Interactioni

Subunit structurei

Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary protein SecDF. Other proteins may also be involved (By similarity).By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015111.

Structurei

3D structure databases

ProteinModelPortaliO32047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 416416SecDAdd
BLAST
Regioni446 – 737292SecFAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the SecD/SecF family. SecD subfamily.Curated
In the C-terminal section; belongs to the SecD/SecF family. SecF subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3D. Bacteria.
COG0341. LUCA.
COG0342. LUCA.
HOGENOMiHOG000018636.
InParanoidiO32047.
KOiK12257.
OMAiAVPLWGM.
OrthoDBiEOG6N0HJT.
PhylomeDBiO32047.

Family and domain databases

HAMAPiMF_01463_B. SecD_B.
MF_01464_B. SecF_B.
InterProiIPR005791. SecD.
IPR022813. SecD/SecF_arch_bac.
IPR022645. SecD/SecF_bac.
IPR022646. SecD/SecF_CS.
IPR005665. SecF_bac.
[Graphical view]
PfamiPF07549. Sec_GG. 1 hit.
PF02355. SecD_SecF. 2 hits.
[Graphical view]
PRINTSiPR01755. SECFTRNLCASE.
TIGRFAMsiTIGR00916. 2A0604s01. 2 hits.
TIGR00966. 3a0501s07. 1 hit.
TIGR01129. secD. 1 hit.

Sequencei

Sequence statusi: Complete.

O32047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKGRLIAFF LFVLLIGTGL GYFTKPAANN ITLGLDLQGG FEVLYDVQPV
60 70 80 90 100
KKGDKITKDV LVSTVEALNR RANVLGVSEP NIQIEGNNRI RVQLAGVTNQ
110 120 130 140 150
NRAREILATE AQLSFRDAND KELLNGADLV ENGAKQTYDS TTNEPIVTIK
160 170 180 190 200
LKDADKFGEV TKKVMKMAPN NQLVIWLDYD KGDSFKKEVQ KEHPKFVSAP
210 220 230 240 250
NVSQELNTTD VKIEGHFTAQ EAKDLASILN AGALPVKLTE KYSTSVGAQF
260 270 280 290 300
GQQALHDTVF AGIVGIAIIF LFMLFYYRLP GLIAVITLSV YIYITLQIFD
310 320 330 340 350
WMNAVLTLPG IAALILGVGM AVDANIITYE RIKEELKLGK SVRSAFRSGN
360 370 380 390 400
RRSFATIFDA NITTIIAAVV LFIFGTSSVK GFATMLILSI LTSFITAVFL
410 420 430 440 450
SRFLLALLVE SRWLDRKKGW FGVNKKHIMD IQDTDENTEP HTPFQKWDFT
460 470 480 490 500
SKRKYFFIFS SAVTVAGIII LLVFRLNLGI DFASGARIEV QSDHKLTTEQ
510 520 530 540 550
VEKDFESLGM DPDTVVLSGE KSNIGVARFV GVPDKETIAK VKTYFKDKYG
560 570 580 590 600
SDPNVSTVSP TVGKELARNA LYAVAIASIG IIIYVSIRFE YKMAIAAIAS
610 620 630 640 650
LLYDAFFIVT FFSITRLEVD VTFIAAILTI IGYSINDTIV TFDRVREHMK
660 670 680 690 700
KRKPKTFADL NHIVNLSLQQ TFTRSINTVL TVVIVVVTLL IFGASSITNF
710 720 730
SIALLVGLLT GVYSSLYIAA QIWLAWKGRE LKKDSAQ
Length:737
Mass (Da):81,654
Last modified:August 1, 1999 - v2
Checksum:i54C397E939779177
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024506 Genomic DNA. Translation: AAC31122.1.
AL009126 Genomic DNA. Translation: CAB14724.2.
PIRiH69704.
RefSeqiNP_390643.1. NC_000964.3.
WP_003245970.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14724; CAB14724; BSU27650.
GeneIDi937938.
KEGGibsu:BSU27650.
PATRICi18977394. VBIBacSub10457_2886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024506 Genomic DNA. Translation: AAC31122.1.
AL009126 Genomic DNA. Translation: CAB14724.2.
PIRiH69704.
RefSeqiNP_390643.1. NC_000964.3.
WP_003245970.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO32047.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015111.

Protein family/group databases

TCDBi2.A.6.4.2. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiO32047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14724; CAB14724; BSU27650.
GeneIDi937938.
KEGGibsu:BSU27650.
PATRICi18977394. VBIBacSub10457_2886.

Phylogenomic databases

eggNOGiENOG4105C3D. Bacteria.
COG0341. LUCA.
COG0342. LUCA.
HOGENOMiHOG000018636.
InParanoidiO32047.
KOiK12257.
OMAiAVPLWGM.
OrthoDBiEOG6N0HJT.
PhylomeDBiO32047.

Enzyme and pathway databases

BioCyciBSUB:BSU27650-MONOMER.

Family and domain databases

HAMAPiMF_01463_B. SecD_B.
MF_01464_B. SecF_B.
InterProiIPR005791. SecD.
IPR022813. SecD/SecF_arch_bac.
IPR022645. SecD/SecF_bac.
IPR022646. SecD/SecF_CS.
IPR005665. SecF_bac.
[Graphical view]
PfamiPF07549. Sec_GG. 1 hit.
PF02355. SecD_SecF. 2 hits.
[Graphical view]
PRINTSiPR01755. SECFTRNLCASE.
TIGRFAMsiTIGR00916. 2A0604s01. 2 hits.
TIGR00966. 3a0501s07. 1 hit.
TIGR01129. secD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins."
    Bolhuis A., Broekhuizen C.P., Sorokin A., van Roosmalen M.L., Venema G., Bron S., Quax W.J., van Dijl J.M.
    J. Biol. Chem. 273:21217-21224(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PROTEIN SECRETION, TOPOLOGY, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Translocation of proteins across the cell envelope of Gram-positive bacteria."
    van Wely K.H.M., Swaving J., Freudl R., Driessen A.J.M.
    FEMS Microbiol. Rev. 25:437-454(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  4. "Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa."
    Rubio A., Jiang X., Pogliano K.
    J. Bacteriol. 187:5000-5002(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: 168 / PY79.

Entry informationi

Entry nameiSECDF_BACSU
AccessioniPrimary (citable) accession number: O32047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: August 1, 1999
Last modified: February 17, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.