Reviewed, UniProtKB/Swiss-Prot O32041 (YRVJ_BACSU)

Names and origin

Protein names

Recommended name:
    Putative N-acetylmuramoyl-L-alanine amidase yrvJ
    EC=3.5.1.28

Gene names

Name:	yrvJ
Ordered Locus Names:	BSU27580

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Probably involved in cell-wall metabolism By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted › cell wall By similarity.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 518	491	Putative N-acetylmuramoyl-L-alanine amidase yrvJ		PRO_0000360805

Sequences

	Sequence		Length	Mass (Da)
	O32041-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8FE642A3D7724135	FASTA	518	55,501
	10 20 30 40 50 60 MNKKYFVLIV CIIFTSALFP TFSSVTAAQG EAVIATDEMN VRSGPGLSYG ITAEVKKGER 70 80 90 100 110 120 YPILKEDGDW VQIQLGSGEK GWVVSWLITK EDQASTSSSG SSDTVTSTDP DLRMRSGPGT 130 140 150 160 170 180 SYEVIGKFPQ GSQASVIDKD SGWIKISYHS ATGWVSSEYV TSGGSSSASD ESDQTEDSGA 190 200 210 220 230 240 STTGTVGVSS LNVRASASHD AAIITKLDRG TKLTVLNEKN GWAHIEVNGL KGWVASHYLL 250 260 270 280 290 300 TSSVPADDSA NAGSSSSAKK AYIMYGGTNL RSDASTSASI VERAAKGDSY TITGSKGSWY 310 320 330 340 350 360 EIKLDNGQTA YVANWVVQTS KSAEEAGEPP VSDSPSGNGS LNNKTIIVDP GHGGKDSGTI 370 380 390 400 410 420 GYSGKFEKNL TIKTAKLLAS KLRSAGADVY VTRQDDTFVS LQSRVSTSHY RNADAFISIH 430 440 450 460 470 480 YDSYADTSTR GSTAYYYSPA KDQELASDVH SEVVKRSSIP DRGVLFGDYY VLRENRQPAM 490 500 510 LYELGYVSHP QEEAIVHSNS YQEKVTDGIE SGLEKYFQ

References

[1]

"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB14717.1.
PIR	B69981.
RefSeq	NP_390636.1.
3D structure databases
HSSP	HSSP built from PDB template 1NQM based on UniProtKB P22629.
ModBase	Search...
Genome annotation databases
GeneID	937544.
GenomeReviews	Gene locus BSU27580 in contig AL009126_GR.
KEGG	bsu:BSU27580.
NMPDR	fig|224308.1.peg.2761.
Organism-specific databases
SubtiList	BG13806. yrvJ. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O32041.
OMA	YVANWVV.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2754-MON.
Family and domain databases
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. IPR017293. N-acetylmuramoyl-L-ala_amidase. IPR013247. SH3_3. IPR003646. SH3_bac. [Graphical view]
Gene3D	G3DSA:3.40.630.40. Cell_wall_OHase/autolysin_cat. 1 hit.
Pfam	PF01520. Amidase_3. 1 hit. PF08239. SH3_3. 4 hits. [Graphical view]
PIRSF	PIRSF037846. Autolysin_YrvJ_prd. 1 hit.
SMART	SM00646. Ami_3. 1 hit. SM00287. SH3b. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YRVJ_BACSU

Accession

Primary (citable) accession number: O32041

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)