Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O32032

- PBPI_BACSU

UniProt

O32032 - PBPI_BACSU

Protein

Penicillin-binding protein 4B

Gene

pbpI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Penicillin-binding protein with an unknown catalytic activity. Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active site of these proteins, thereby interrupting normal cell wall synthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei304 – 3041Acyl-ester intermediateBy similarity

    GO - Molecular functioni

    1. penicillin binding Source: InterPro
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Sporulation

    Enzyme and pathway databases

    BioCyciBSUB:BSU27310-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penicillin-binding protein 4B
    Short name:
    PBP-4B
    Short name:
    PBP4b
    Alternative name(s):
    Penicillin-binding protein I
    Gene namesi
    Name:pbpI
    Synonyms:yrrR
    Ordered Locus Names:BSU27310
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU27310. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Spores have normal morphology, heat-resistance, cortex structure, and germination and outgrowth properties.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Penicillin-binding protein 4BPRO_0000360667Add
    BLAST

    Proteomic databases

    PaxDbiO32032.

    Expressioni

    Developmental stagei

    Sporulation specific. Expression starts 1 to 2 hours after the initiation of sporulation. Found only in mother cells.

    Interactioni

    Protein-protein interaction databases

    IntActiO32032. 1 interaction.
    STRINGi224308.BSU27310.

    Structurei

    3D structure databases

    ProteinModelPortaliO32032.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 584552ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the transpeptidase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0768.
    HOGENOMiHOG000097173.
    OMAiKWFAGYF.
    OrthoDBiEOG6N0HHV.
    PhylomeDBiO32032.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    O32032-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKISKRMKLA VIAFLIVFFL LLLRLAEIQL FFTESFSKKK INLIQESVKQ    50
    RTEEVLISDG RGSFLDRNGR ALTGQSEPAV VLFPFLLTQD WPIKKVADIL 100
    GMSEDDLRQT LGQAKKPVIL QQKKIKTLSK QSITKINSLK YPGIYGVYME 150
    NEDKPSLASH TIGSTNQDPA LLRKKYPDKE SLPITTEIGT TGLERTFDEF 200
    LLPEQDTKLL YHVDGKGNPL FGMDVKYTAE ANTFYPLQIK TTIDQSIQKA 250
    MEEVLDEQGL KKGGAVLLDI ENSSVLGIVS KPDADVSRQN TLQNYMLTPI 300
    YPGSVFKTVI AAAAIENNMV KPSQTFNCNL NLYGEPGDDK GTLSFDESFA 350
    QSCNYTFTSL AEQLMKKDSS VIEDMSEKLA LTDRAGWEGK LYHETDFRQL 400
    YNEKSGVIWG DEKDKSVKKA IAQTAIGQKN VKVTPLEVAN MMATIARGGE 450
    KRQVKIAEQI EYKNGTTLVT FKDQKLKGET IDKYTSQQLQ KILRRVVESP 500
    SGTGRRFQDL PYTVAGKSGT AQTGKLSKEK ETLYEKWFAG YFPADKPKYA 550
    LVVLHMDTPG DKALTNSVFY DIVKKVHEIE INQK 584
    Length:584
    Mass (Da):65,522
    Last modified:January 1, 1998 - v1
    Checksum:i889140C9E9D69A13
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB14673.1.
    PIRiA69980.
    RefSeqiNP_390609.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14673; CAB14673; BSU27310.
    GeneIDi936016.
    KEGGibsu:BSU27310.
    PATRICi18977318. VBIBacSub10457_2848.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB14673.1 .
    PIRi A69980.
    RefSeqi NP_390609.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali O32032.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O32032. 1 interaction.
    STRINGi 224308.BSU27310.

    Proteomic databases

    PaxDbi O32032.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14673 ; CAB14673 ; BSU27310 .
    GeneIDi 936016.
    KEGGi bsu:BSU27310.
    PATRICi 18977318. VBIBacSub10457_2848.

    Organism-specific databases

    GenoListi BSU27310. [Micado ]

    Phylogenomic databases

    eggNOGi COG0768.
    HOGENOMi HOG000097173.
    OMAi KWFAGYF.
    OrthoDBi EOG6N0HHV.
    PhylomeDBi O32032.

    Enzyme and pathway databases

    BioCyci BSUB:BSU27310-MONOMER.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    2. "A mother cell-specific class B penicillin-binding protein, PBP4b, in Bacillus subtilis."
      Wei Y., McPherson D.C., Popham D.L.
      J. Bacteriol. 186:258-261(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPBPI_BACSU
    AccessioniPrimary (citable) accession number: O32032
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3