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O31852

- CWLS_BACSU

UniProt

O31852 - CWLS_BACSU

Protein

D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS

Gene

cwlS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Probably functions as a cell separation enzyme in addition to LytE and LytF.1 Publication

    Catalytic activityi

    Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A2pm) in 7-(L-Ala-gamma-D-Glu)-meso-A2pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A2pm. It is required that the D-terminal amino and carboxy groups of meso-A2pm are unsubstituted.

    pH dependencei

    Optimum pH is 6-7 without NaCl at 37 degrees Celsius.1 Publication

    Temperature dependencei

    Optimum temperature is 48 degrees Celsius in 50 mM MOPS-NaOH buffer (pH 7.0) without NaCl.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei326 – 3261NucleophileBy similarity
    Active sitei375 – 3751Proton acceptorBy similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciBSUB:BSU19410-MONOMER.

    Protein family/group databases

    MEROPSiC40.008.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS (EC:3.4.19.11)
    Alternative name(s):
    Cell wall lytic enzyme associated with cell separation
    Gene namesi
    Name:cwlS
    Synonyms:yojL
    Ordered Locus Names:BSU19410
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU19410. [Micado]

    Subcellular locationi

    Cell surface 1 Publication
    Note: Localized at cell separation sites and cell poles during late vegetative phase.

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell

    Pathology & Biotechi

    Disruption phenotypei

    The cell shape is not significantly different from that of the wild-type.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 414388D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlSPRO_0000019765Add
    BLAST

    Proteomic databases

    PaxDbiO31852.

    Expressioni

    Developmental stagei

    Transcribed during the late vegetative and early stationary phases.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU19410.

    Structurei

    3D structure databases

    ProteinModelPortaliO31852.
    SMRiO31852. Positions 27-73, 88-132, 224-269, 298-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati29 – 7143LysM 1Add
    BLAST
    Repeati90 – 13243LysM 2Add
    BLAST
    Repeati159 – 20143LysM 3Add
    BLAST
    Repeati227 – 26943LysM 4Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi135 – 1406Poly-Ser

    Domaini

    LysM repeats are thought to be involved in peptidoglycan binding.

    Sequence similaritiesi

    Belongs to the peptidase C40 family.Curated
    Contains 4 LysM repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG0791.
    HOGENOMiHOG000229978.
    OMAiSTHIRVD.
    OrthoDBiEOG6GTZPF.
    PhylomeDBiO31852.

    Family and domain databases

    Gene3Di3.10.350.10. 4 hits.
    3.90.1720.10. 1 hit.
    InterProiIPR018392. LysM_dom.
    IPR000064. NLP_P60_dom.
    [Graphical view]
    PfamiPF01476. LysM. 4 hits.
    PF00877. NLPC_P60. 1 hit.
    [Graphical view]
    SMARTiSM00257. LysM. 4 hits.
    [Graphical view]
    SUPFAMiSSF54106. SSF54106. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O31852-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKIVAGLA VSAVVGSSMA AAPAEAKTIK VKSGDSLWKL SRQYDTTISA    50
    LKSENKLKST VLYVGQSLKV PESSKKSTTS PSNSSKTSTY TVAYGDSLWM 100
    IAKNHKMSVS ELKSLNSLSS DLIRPGQKLK IKGTSSSSGS NGSKKNSGSN 150
    SSGSSKSTYT VKLGDSLWKI ANSLNMTVAE LKTLNGLTSD TLYPKQVLKI 200
    KGSSSPKNGN SGSKKPSNSN PSKTTTYKVK AGDSLWKIAN RLGVTVQSIR 250
    DKNNLSSDVL QIGQVLTISG ASKSNPSNPT KPTKPKDNSG SNIQIGSKID 300
    RMITEAKKYV GVPYRWGGNT PAGFDCSGFI YYLINNVSSI SRLSTAGYWN 350
    VMQKVSQPSV GDFVFFTTYK SGPSHMGIYL GGGDFIHASS SGVDISNLSN 400
    SYWKQRYLGA RSYF 414
    Length:414
    Mass (Da):44,235
    Last modified:January 1, 1998 - v1
    Checksum:i25C5B188221B2F87
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF026147 Genomic DNA. Translation: AAC17860.1.
    AL009126 Genomic DNA. Translation: CAB13833.1.
    PIRiA69907.
    RefSeqiNP_389823.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13833; CAB13833; BSU19410.
    GeneIDi939459.
    KEGGibsu:BSU19410.
    PATRICi18975733. VBIBacSub10457_2057.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF026147 Genomic DNA. Translation: AAC17860.1 .
    AL009126 Genomic DNA. Translation: CAB13833.1 .
    PIRi A69907.
    RefSeqi NP_389823.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali O31852.
    SMRi O31852. Positions 27-73, 88-132, 224-269, 298-414.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU19410.

    Protein family/group databases

    MEROPSi C40.008.

    Proteomic databases

    PaxDbi O31852.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13833 ; CAB13833 ; BSU19410 .
    GeneIDi 939459.
    KEGGi bsu:BSU19410.
    PATRICi 18975733. VBIBacSub10457_2057.

    Organism-specific databases

    GenoListi BSU19410. [Micado ]

    Phylogenomic databases

    eggNOGi COG0791.
    HOGENOMi HOG000229978.
    OMAi STHIRVD.
    OrthoDBi EOG6GTZPF.
    PhylomeDBi O31852.

    Enzyme and pathway databases

    BioCyci BSUB:BSU19410-MONOMER.

    Family and domain databases

    Gene3Di 3.10.350.10. 4 hits.
    3.90.1720.10. 1 hit.
    InterProi IPR018392. LysM_dom.
    IPR000064. NLP_P60_dom.
    [Graphical view ]
    Pfami PF01476. LysM. 4 hits.
    PF00877. NLPC_P60. 1 hit.
    [Graphical view ]
    SMARTi SM00257. LysM. 4 hits.
    [Graphical view ]
    SUPFAMi SSF54106. SSF54106. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the Bacillus subtilis 168 chromosome region between the sspC and odhA loci (184 degrees-180 degrees)."
      Ghim S.-Y., Choi S.-K., Shin B.-S., Jeong Y.-M., Sorokin A., Ehrlich S.D., Park S.-H.
      DNA Res. 5:195-201(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "A new D,L-endopeptidase gene product, YojL (renamed CwlS), plays a role in cell separation with LytE and LytF in Bacillus subtilis."
      Fukushima T., Afkham A., Kurosawa S., Tanabe T., Yamamoto H., Sekiguchi J.
      J. Bacteriol. 188:5541-5550(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
      Strain: 168.

    Entry informationi

    Entry nameiCWLS_BACSU
    AccessioniPrimary (citable) accession number: O31852
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3