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Protein

D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS

Gene

cwlS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably functions as a cell separation enzyme in addition to LytE and LytF.1 Publication

Catalytic activityi

Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A2pm) in 7-(L-Ala-gamma-D-Glu)-meso-A2pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A2pm. It is required that the D-terminal amino and carboxy groups of meso-A2pm are unsubstituted.

pH dependencei

Optimum pH is 6-7 without NaCl at 37 degrees Celsius.1 Publication

Temperature dependencei

Optimum temperature is 48 degrees Celsius in 50 mM MOPS-NaOH buffer (pH 7.0) without NaCl.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei326NucleophileBy similarity1
Active sitei375Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciBSUB:BSU19410-MONOMER

Protein family/group databases

CAZyiCBM50 Carbohydrate-Binding Module Family 50
MEROPSiC40.008

Names & Taxonomyi

Protein namesi
Recommended name:
D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS (EC:3.4.19.11)
Alternative name(s):
Cell wall lytic enzyme associated with cell separation
Gene namesi
Name:cwlS
Synonyms:yojL
Ordered Locus Names:BSU19410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell surface 1 Publication
  • Note: Localized at cell separation sites and cell poles during late vegetative phase.

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

The cell shape is not significantly different from that of the wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001976527 – 414D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlSAdd BLAST388

Proteomic databases

PaxDbiO31852
PRIDEiO31852

Expressioni

Developmental stagei

Transcribed during the late vegetative and early stationary phases.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010716

Structurei

3D structure databases

ProteinModelPortaliO31852
SMRiO31852
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 70LysM 1PROSITE-ProRule annotationAdd BLAST44
Domaini88 – 131LysM 2PROSITE-ProRule annotationAdd BLAST44
Domaini157 – 200LysM 3PROSITE-ProRule annotationAdd BLAST44
Domaini225 – 268LysM 4PROSITE-ProRule annotationAdd BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi135 – 140Poly-Ser6

Domaini

LysM domains are thought to be involved in peptidoglycan binding.

Sequence similaritiesi

Belongs to the peptidase C40 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105K4H Bacteria
COG0791 LUCA
COG1388 LUCA
HOGENOMiHOG000229978
InParanoidiO31852
KOiK19220
OMAiRMITEAK
PhylomeDBiO31852

Family and domain databases

CDDicd00118 LysM, 4 hits
Gene3Di3.10.350.10, 4 hits
InterProiView protein in InterPro
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR000064 NLP_P60_dom
PfamiView protein in Pfam
PF01476 LysM, 4 hits
PF00877 NLPC_P60, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 4 hits
PROSITEiView protein in PROSITE
PS51782 LYSM, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O31852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKIVAGLA VSAVVGSSMA AAPAEAKTIK VKSGDSLWKL SRQYDTTISA
60 70 80 90 100
LKSENKLKST VLYVGQSLKV PESSKKSTTS PSNSSKTSTY TVAYGDSLWM
110 120 130 140 150
IAKNHKMSVS ELKSLNSLSS DLIRPGQKLK IKGTSSSSGS NGSKKNSGSN
160 170 180 190 200
SSGSSKSTYT VKLGDSLWKI ANSLNMTVAE LKTLNGLTSD TLYPKQVLKI
210 220 230 240 250
KGSSSPKNGN SGSKKPSNSN PSKTTTYKVK AGDSLWKIAN RLGVTVQSIR
260 270 280 290 300
DKNNLSSDVL QIGQVLTISG ASKSNPSNPT KPTKPKDNSG SNIQIGSKID
310 320 330 340 350
RMITEAKKYV GVPYRWGGNT PAGFDCSGFI YYLINNVSSI SRLSTAGYWN
360 370 380 390 400
VMQKVSQPSV GDFVFFTTYK SGPSHMGIYL GGGDFIHASS SGVDISNLSN
410
SYWKQRYLGA RSYF
Length:414
Mass (Da):44,235
Last modified:January 1, 1998 - v1
Checksum:i25C5B188221B2F87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026147 Genomic DNA Translation: AAC17860.1
AL009126 Genomic DNA Translation: CAB13833.1
PIRiA69907
RefSeqiNP_389823.1, NC_000964.3
WP_004399265.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB13833; CAB13833; BSU19410
GeneIDi939459
KEGGibsu:BSU19410
PATRICifig|224308.179.peg.2122

Similar proteinsi

Entry informationi

Entry nameiCWLS_BACSU
AccessioniPrimary (citable) accession number: O31852
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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