Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O31852 (CWLS_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS

EC=3.4.19.11
Alternative name(s):
Cell wall lytic enzyme associated with cell separation
Gene names
Name:cwlS
Synonyms:yojL
Ordered Locus Names:BSU19410
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably functions as a cell separation enzyme in addition to LytE and LytF. Ref.3

Catalytic activity

Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A2pm) in 7-(L-Ala-gamma-D-Glu)-meso-A2pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A2pm. It is required that the D-terminal amino and carboxy groups of meso-A2pm are unsubstituted.

Subcellular location

Cell surface. Note: Localized at cell separation sites and cell poles during late vegetative phase. Ref.3

Developmental stage

Transcribed during the late vegetative and early stationary phases. Ref.3

Domain

LysM repeats are thought to be involved in peptidoglycan binding.

Disruption phenotype

The cell shape is not significantly different from that of the wild-type. Ref.3

Sequence similarities

Belongs to the peptidase C40 family.

Contains 4 LysM repeats.

Biophysicochemical properties

pH dependence:

Optimum pH is 6-7 without NaCl at 37 degrees Celsius. Ref.3

Temperature dependence:

Optimum temperature is 48 degrees Celsius in 50 mM MOPS-NaOH buffer (pH 7.0) without NaCl.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   DomainRepeat
Signal
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell wall macromolecule catabolic process

Inferred from electronic annotation. Source: InterPro

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell surface

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 414388D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS
PRO_0000019765

Regions

Repeat29 – 7143LysM 1
Repeat90 – 13243LysM 2
Repeat159 – 20143LysM 3
Repeat227 – 26943LysM 4
Compositional bias135 – 1406Poly-Ser

Sites

Active site3261Nucleophile By similarity
Active site3751Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
O31852 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 25C5B188221B2F87

FASTA41444,235
        10         20         30         40         50         60 
MKKKIVAGLA VSAVVGSSMA AAPAEAKTIK VKSGDSLWKL SRQYDTTISA LKSENKLKST 

        70         80         90        100        110        120 
VLYVGQSLKV PESSKKSTTS PSNSSKTSTY TVAYGDSLWM IAKNHKMSVS ELKSLNSLSS 

       130        140        150        160        170        180 
DLIRPGQKLK IKGTSSSSGS NGSKKNSGSN SSGSSKSTYT VKLGDSLWKI ANSLNMTVAE 

       190        200        210        220        230        240 
LKTLNGLTSD TLYPKQVLKI KGSSSPKNGN SGSKKPSNSN PSKTTTYKVK AGDSLWKIAN 

       250        260        270        280        290        300 
RLGVTVQSIR DKNNLSSDVL QIGQVLTISG ASKSNPSNPT KPTKPKDNSG SNIQIGSKID 

       310        320        330        340        350        360 
RMITEAKKYV GVPYRWGGNT PAGFDCSGFI YYLINNVSSI SRLSTAGYWN VMQKVSQPSV 

       370        380        390        400        410 
GDFVFFTTYK SGPSHMGIYL GGGDFIHASS SGVDISNLSN SYWKQRYLGA RSYF 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the Bacillus subtilis 168 chromosome region between the sspC and odhA loci (184 degrees-180 degrees)."
Ghim S.-Y., Choi S.-K., Shin B.-S., Jeong Y.-M., Sorokin A., Ehrlich S.D., Park S.-H.
DNA Res. 5:195-201(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"A new D,L-endopeptidase gene product, YojL (renamed CwlS), plays a role in cell separation with LytE and LytF in Bacillus subtilis."
Fukushima T., Afkham A., Kurosawa S., Tanabe T., Yamamoto H., Sekiguchi J.
J. Bacteriol. 188:5541-5550(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF026147 Genomic DNA. Translation: AAC17860.1.
AL009126 Genomic DNA. Translation: CAB13833.1.
PIRA69907.
RefSeqNP_389823.1. NC_000964.3.

3D structure databases

ProteinModelPortalO31852.
SMRO31852. Positions 27-73, 88-132, 224-269, 298-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU19410.

Protein family/group databases

MEROPSC40.008.

Proteomic databases

PaxDbO31852.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13833; CAB13833; BSU19410.
GeneID939459.
KEGGbsu:BSU19410.
PATRIC18975733. VBIBacSub10457_2057.

Organism-specific databases

GenoListBSU19410. [Micado]

Phylogenomic databases

eggNOGCOG0791.
HOGENOMHOG000229978.
OMAGSKIDRM.
OrthoDBEOG6GTZPF.
ProtClustDBCLSK886953.

Enzyme and pathway databases

BioCycBSUB:BSU19410-MONOMER.

Family and domain databases

Gene3D3.10.350.10. 4 hits.
3.90.1720.10. 1 hit.
InterProIPR018392. LysM_dom.
IPR000064. NLP_P60_dom.
[Graphical view]
PfamPF01476. LysM. 4 hits.
PF00877. NLPC_P60. 1 hit.
[Graphical view]
SMARTSM00257. LysM. 4 hits.
[Graphical view]
SUPFAMSSF54106. SSF54106. 4 hits.
ProtoNetSearch...

Entry information

Entry nameCWLS_BACSU
AccessionPrimary (citable) accession number: O31852
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList