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Protein

D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS

Gene

cwlS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably functions as a cell separation enzyme in addition to LytE and LytF.1 Publication

Catalytic activityi

Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A2pm) in 7-(L-Ala-gamma-D-Glu)-meso-A2pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A2pm. It is required that the D-terminal amino and carboxy groups of meso-A2pm are unsubstituted.

pH dependencei

Optimum pH is 6-7 without NaCl at 37 degrees Celsius.1 Publication

Temperature dependencei

Optimum temperature is 48 degrees Celsius in 50 mM MOPS-NaOH buffer (pH 7.0) without NaCl.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei326 – 3261NucleophileBy similarity
Active sitei375 – 3751Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciBSUB:BSU19410-MONOMER.

Protein family/group databases

CAZyiCBM50. Carbohydrate-Binding Module Family 50.
MEROPSiC40.008.

Names & Taxonomyi

Protein namesi
Recommended name:
D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS (EC:3.4.19.11)
Alternative name(s):
Cell wall lytic enzyme associated with cell separation
Gene namesi
Name:cwlS
Synonyms:yojL
Ordered Locus Names:BSU19410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell surface 1 Publication

  • Note: Localized at cell separation sites and cell poles during late vegetative phase.

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

The cell shape is not significantly different from that of the wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 414388D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlSPRO_0000019765Add
BLAST

Proteomic databases

PaxDbiO31852.

Expressioni

Developmental stagei

Transcribed during the late vegetative and early stationary phases.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010716.

Structurei

3D structure databases

ProteinModelPortaliO31852.
SMRiO31852. Positions 27-73, 88-132, 224-269, 298-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 7044LysM 1PROSITE-ProRule annotationAdd
BLAST
Domaini88 – 13144LysM 2PROSITE-ProRule annotationAdd
BLAST
Domaini157 – 20044LysM 3PROSITE-ProRule annotationAdd
BLAST
Domaini225 – 26844LysM 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi135 – 1406Poly-Ser

Domaini

LysM domains are thought to be involved in peptidoglycan binding.

Sequence similaritiesi

Belongs to the peptidase C40 family.Curated
Contains 4 LysM domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105K4H. Bacteria.
COG0791. LUCA.
COG1388. LUCA.
HOGENOMiHOG000229978.
InParanoidiO31852.
KOiK19220.
OMAiYYLINNV.
PhylomeDBiO31852.

Family and domain databases

Gene3Di3.10.350.10. 4 hits.
3.90.1720.10. 1 hit.
InterProiIPR018392. LysM_dom.
IPR000064. NLP_P60_dom.
[Graphical view]
PfamiPF01476. LysM. 4 hits.
PF00877. NLPC_P60. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 4 hits.
[Graphical view]
SUPFAMiSSF54106. SSF54106. 4 hits.
PROSITEiPS51782. LYSM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O31852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKIVAGLA VSAVVGSSMA AAPAEAKTIK VKSGDSLWKL SRQYDTTISA
60 70 80 90 100
LKSENKLKST VLYVGQSLKV PESSKKSTTS PSNSSKTSTY TVAYGDSLWM
110 120 130 140 150
IAKNHKMSVS ELKSLNSLSS DLIRPGQKLK IKGTSSSSGS NGSKKNSGSN
160 170 180 190 200
SSGSSKSTYT VKLGDSLWKI ANSLNMTVAE LKTLNGLTSD TLYPKQVLKI
210 220 230 240 250
KGSSSPKNGN SGSKKPSNSN PSKTTTYKVK AGDSLWKIAN RLGVTVQSIR
260 270 280 290 300
DKNNLSSDVL QIGQVLTISG ASKSNPSNPT KPTKPKDNSG SNIQIGSKID
310 320 330 340 350
RMITEAKKYV GVPYRWGGNT PAGFDCSGFI YYLINNVSSI SRLSTAGYWN
360 370 380 390 400
VMQKVSQPSV GDFVFFTTYK SGPSHMGIYL GGGDFIHASS SGVDISNLSN
410
SYWKQRYLGA RSYF
Length:414
Mass (Da):44,235
Last modified:January 1, 1998 - v1
Checksum:i25C5B188221B2F87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026147 Genomic DNA. Translation: AAC17860.1.
AL009126 Genomic DNA. Translation: CAB13833.1.
PIRiA69907.
RefSeqiNP_389823.1. NC_000964.3.
WP_004399265.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB13833; CAB13833; BSU19410.
GeneIDi939459.
KEGGibsu:BSU19410.
PATRICi18975733. VBIBacSub10457_2057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026147 Genomic DNA. Translation: AAC17860.1.
AL009126 Genomic DNA. Translation: CAB13833.1.
PIRiA69907.
RefSeqiNP_389823.1. NC_000964.3.
WP_004399265.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO31852.
SMRiO31852. Positions 27-73, 88-132, 224-269, 298-414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010716.

Protein family/group databases

CAZyiCBM50. Carbohydrate-Binding Module Family 50.
MEROPSiC40.008.

Proteomic databases

PaxDbiO31852.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13833; CAB13833; BSU19410.
GeneIDi939459.
KEGGibsu:BSU19410.
PATRICi18975733. VBIBacSub10457_2057.

Phylogenomic databases

eggNOGiENOG4105K4H. Bacteria.
COG0791. LUCA.
COG1388. LUCA.
HOGENOMiHOG000229978.
InParanoidiO31852.
KOiK19220.
OMAiYYLINNV.
PhylomeDBiO31852.

Enzyme and pathway databases

BioCyciBSUB:BSU19410-MONOMER.

Family and domain databases

Gene3Di3.10.350.10. 4 hits.
3.90.1720.10. 1 hit.
InterProiIPR018392. LysM_dom.
IPR000064. NLP_P60_dom.
[Graphical view]
PfamiPF01476. LysM. 4 hits.
PF00877. NLPC_P60. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 4 hits.
[Graphical view]
SUPFAMiSSF54106. SSF54106. 4 hits.
PROSITEiPS51782. LYSM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCWLS_BACSU
AccessioniPrimary (citable) accession number: O31852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.