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O31852

- CWLS_BACSU

UniProt

O31852 - CWLS_BACSU

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Protein

D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS

Gene

cwlS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Probably functions as a cell separation enzyme in addition to LytE and LytF.1 Publication

Catalytic activityi

Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A2pm) in 7-(L-Ala-gamma-D-Glu)-meso-A2pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A2pm. It is required that the D-terminal amino and carboxy groups of meso-A2pm are unsubstituted.

pH dependencei

Optimum pH is 6-7 without NaCl at 37 degrees Celsius.1 Publication

Temperature dependencei

Optimum temperature is 48 degrees Celsius in 50 mM MOPS-NaOH buffer (pH 7.0) without NaCl.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei326 – 3261NucleophileBy similarity
Active sitei375 – 3751Proton acceptorBy similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciBSUB:BSU19410-MONOMER.

Protein family/group databases

MEROPSiC40.008.

Names & Taxonomyi

Protein namesi
Recommended name:
D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS (EC:3.4.19.11)
Alternative name(s):
Cell wall lytic enzyme associated with cell separation
Gene namesi
Name:cwlS
Synonyms:yojL
Ordered Locus Names:BSU19410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU19410. [Micado]

Subcellular locationi

Cell surface 1 Publication
Note: Localized at cell separation sites and cell poles during late vegetative phase.

Pathology & Biotechi

Disruption phenotypei

The cell shape is not significantly different from that of the wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 414388D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlSPRO_0000019765Add
BLAST

Proteomic databases

PaxDbiO31852.

Expressioni

Developmental stagei

Transcribed during the late vegetative and early stationary phases.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU19410.

Structurei

3D structure databases

ProteinModelPortaliO31852.
SMRiO31852. Positions 27-73, 88-132, 224-269, 298-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati29 – 7143LysM 1Add
BLAST
Repeati90 – 13243LysM 2Add
BLAST
Repeati159 – 20143LysM 3Add
BLAST
Repeati227 – 26943LysM 4Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi135 – 1406Poly-Ser

Domaini

LysM repeats are thought to be involved in peptidoglycan binding.

Sequence similaritiesi

Belongs to the peptidase C40 family.Curated
Contains 4 LysM repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG0791.
HOGENOMiHOG000229978.
InParanoidiO31852.
OMAiSTHIRVD.
OrthoDBiEOG6GTZPF.
PhylomeDBiO31852.

Family and domain databases

Gene3Di3.10.350.10. 4 hits.
3.90.1720.10. 1 hit.
InterProiIPR018392. LysM_dom.
IPR000064. NLP_P60_dom.
[Graphical view]
PfamiPF01476. LysM. 4 hits.
PF00877. NLPC_P60. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 4 hits.
[Graphical view]
SUPFAMiSSF54106. SSF54106. 4 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O31852-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKKIVAGLA VSAVVGSSMA AAPAEAKTIK VKSGDSLWKL SRQYDTTISA
60 70 80 90 100
LKSENKLKST VLYVGQSLKV PESSKKSTTS PSNSSKTSTY TVAYGDSLWM
110 120 130 140 150
IAKNHKMSVS ELKSLNSLSS DLIRPGQKLK IKGTSSSSGS NGSKKNSGSN
160 170 180 190 200
SSGSSKSTYT VKLGDSLWKI ANSLNMTVAE LKTLNGLTSD TLYPKQVLKI
210 220 230 240 250
KGSSSPKNGN SGSKKPSNSN PSKTTTYKVK AGDSLWKIAN RLGVTVQSIR
260 270 280 290 300
DKNNLSSDVL QIGQVLTISG ASKSNPSNPT KPTKPKDNSG SNIQIGSKID
310 320 330 340 350
RMITEAKKYV GVPYRWGGNT PAGFDCSGFI YYLINNVSSI SRLSTAGYWN
360 370 380 390 400
VMQKVSQPSV GDFVFFTTYK SGPSHMGIYL GGGDFIHASS SGVDISNLSN
410
SYWKQRYLGA RSYF
Length:414
Mass (Da):44,235
Last modified:January 1, 1998 - v1
Checksum:i25C5B188221B2F87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026147 Genomic DNA. Translation: AAC17860.1.
AL009126 Genomic DNA. Translation: CAB13833.1.
PIRiA69907.
RefSeqiNP_389823.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13833; CAB13833; BSU19410.
GeneIDi939459.
KEGGibsu:BSU19410.
PATRICi18975733. VBIBacSub10457_2057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026147 Genomic DNA. Translation: AAC17860.1 .
AL009126 Genomic DNA. Translation: CAB13833.1 .
PIRi A69907.
RefSeqi NP_389823.1. NC_000964.3.

3D structure databases

ProteinModelPortali O31852.
SMRi O31852. Positions 27-73, 88-132, 224-269, 298-414.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU19410.

Protein family/group databases

MEROPSi C40.008.

Proteomic databases

PaxDbi O31852.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13833 ; CAB13833 ; BSU19410 .
GeneIDi 939459.
KEGGi bsu:BSU19410.
PATRICi 18975733. VBIBacSub10457_2057.

Organism-specific databases

GenoListi BSU19410. [Micado ]

Phylogenomic databases

eggNOGi COG0791.
HOGENOMi HOG000229978.
InParanoidi O31852.
OMAi STHIRVD.
OrthoDBi EOG6GTZPF.
PhylomeDBi O31852.

Enzyme and pathway databases

BioCyci BSUB:BSU19410-MONOMER.

Family and domain databases

Gene3Di 3.10.350.10. 4 hits.
3.90.1720.10. 1 hit.
InterProi IPR018392. LysM_dom.
IPR000064. NLP_P60_dom.
[Graphical view ]
Pfami PF01476. LysM. 4 hits.
PF00877. NLPC_P60. 1 hit.
[Graphical view ]
SMARTi SM00257. LysM. 4 hits.
[Graphical view ]
SUPFAMi SSF54106. SSF54106. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the Bacillus subtilis 168 chromosome region between the sspC and odhA loci (184 degrees-180 degrees)."
    Ghim S.-Y., Choi S.-K., Shin B.-S., Jeong Y.-M., Sorokin A., Ehrlich S.D., Park S.-H.
    DNA Res. 5:195-201(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "A new D,L-endopeptidase gene product, YojL (renamed CwlS), plays a role in cell separation with LytE and LytF in Bacillus subtilis."
    Fukushima T., Afkham A., Kurosawa S., Tanabe T., Yamamoto H., Sekiguchi J.
    J. Bacteriol. 188:5541-5550(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: 168.

Entry informationi

Entry nameiCWLS_BACSU
AccessioniPrimary (citable) accession number: O31852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3