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Protein

Plipastatin synthase subunit E

Gene

ppsE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is a multifunctional enzyme, able to activate and polymerize the amino acid Ile as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The activation sites for this amino acid consist of individual domains.1 Publication

Cofactori

pantetheine 4'-phosphateCuratedNote: Binds 1 phosphopantetheine covalently.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processAntibiotic biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU18300-MONOMER

Protein family/group databases

ESTHERibacsu-PPSE Thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Plipastatin synthase subunit E (EC:2.3.1.-)
Alternative name(s):
Peptide synthase 5
Including the following 1 domains:
ATP-dependent isoleucine adenylase
Short name:
IsoA
Alternative name(s):
Isoleucine activase
Gene namesi
Name:ppsE
Synonyms:pps5
Ordered Locus Names:BSU18300
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003608451 – 1279Plipastatin synthase subunit EAdd BLAST1279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1010O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiO31827
PRIDEiO31827

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010086

Structurei

3D structure databases

ProteinModelPortaliO31827
SMRiO31827
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini975 – 1050CarrierPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 1047Domain (isoleucine-activating)Add BLAST1034
Regioni14 – 317CondensationAdd BLAST304
Regioni503 – 898AdenylationAdd BLAST396
Regioni1068 – 1273ThioesteraseAdd BLAST206

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3319 LUCA
HOGENOMiHOG000229993
InParanoidiO31827
KOiK15668
OMAiAHHIACD
PhylomeDBiO31827

Family and domain databases

Gene3Di1.10.1200.10, 1 hit
3.30.559.10, 2 hits
3.40.50.1820, 2 hits
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR029058 AB_hydrolase
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020459 AMP-binding
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR020806 PKS_PP-bd
IPR020802 PKS_thioesterase
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR001031 Thioesterase
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PF00668 Condensation, 1 hit
PF00550 PP-binding, 1 hit
PF00975 Thioesterase, 1 hit
PRINTSiPR00154 AMPBINDING
SMARTiView protein in SMART
SM00823 PKS_PP, 1 hit
SM00824 PKS_TE, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit

Sequencei

Sequence statusi: Complete.

O31827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKGADTMNT IKKIKNIYPL SHMQEGMLFH SFLRKEEGAY VEQSLFTIKG
60 70 80 90 100
SLSYDWFQRS IQAIIDRHDI FRTVFLPHVP HLSGPRQVVM TEREFHLNSE
110 120 130 140 150
DISHLPTNDQ NEYIERFKEK DKQKGFDLQK DMLMRISLFK TAKDEHVCIW
160 170 180 190 200
SHHHILMDGW CLGIVMQEFM QIYQSIHAGK PLSLDPVRPY STYISWLTNR
210 220 230 240 250
DKEKAAAYWD TYLKNYSAPS PLPRVSDKET KESYHREDLI FSLNKPLTDK
260 270 280 290 300
LKETAKQHGV TLATLIQAVW GVMLQQYNRT DDVVFGAVVS GRPSEIPGVE
310 320 330 340 350
QMIGLFINTI PIRIKTHQDE TFHELLIRCQ KEMLEAEPFT CQPLFDIQAN
360 370 380 390 400
TALKQELIDH IIVFENYPLQ QKIADSADQT DSPLQIDQVQ VSEQSGYNFN
410 420 430 440 450
LVVAPGEELV IKFSYNAFVY DAAWISCIKR QFTQALSTAA QHPHMPIADF
460 470 480 490 500
SFLDATEKEQ IVTQFNNTKT EYPKNHTIID LFREQAEKTP DHTALVYGNM
510 520 530 540 550
SISYKELDKR SNALARELIQ KGFRKNETAG ILAAHSPEFM ISVLAVLKAG
560 570 580 590 600
GAYLPLDAEL PPERVSFMLE ETQAKMLIVQ KGLEQNAAFS GTCIISDAQG
610 620 630 640 650
LMEENDIPIN ISSSPDDLAY IMYTSGSTGR PKGVMITNRN VVSLVRNSNY
660 670 680 690 700
TSASGDDRFI MTGSISFDAV TFEMFGALLN GASLHIIDKS TMLTPDRFGA
710 720 730 740 750
YLLENDITVL FLTTALFNQL AQVRADMFRG LHTLYVGGEA LSPALMNAVR
760 770 780 790 800
HACPDLALHN IYGPTENTTF STFFEMKRDY AGPIPIGKPI SNSTAYILDT
810 820 830 840 850
KGRLLPIGVP GELCVGGDGV AKGYLNRVDL TNAVFSPHPF LPGERIYRTG
860 870 880 890 900
DLARWLPDGN LEYISRIDRQ MKIRGKRIEP AEIEARLLEM EGVQEAAVTL
910 920 930 940 950
REKDGEAQLY THYVGDHKKT DTDFRADLAR VLPDYMIPQH WVRVERMPLT
960 970 980 990 1000
GNGKIDRSAL PIPENKPAKR QNIILPRNLV EEELANIWKQ VLGVNTISID
1010 1020 1030 1040 1050
DDFFAIGGHS LRALQVIHTL KHQQNIDIPI DFLFEHPTIA QLAEKLYSKQ
1060 1070 1080 1090 1100
LTAANEQHVI KLNQHGAQNL FCFPPISGFG IYFKDLALLL NEKAAVYGFH
1110 1120 1130 1140 1150
FIEQDTRIEQ YVNCMTDIQP EGPYVLLGYS AGGNLAFEVA QAMERKGLEV
1160 1170 1180 1190 1200
SDFIIVDAYL KEQPLPIDTG NDESAAYLPE AVREKVMKKK RNYQEYWAQL
1210 1220 1230 1240 1250
LNEGHIKASI HFIEAGIHPE TSGHTGLTKW EGACGNYSEY TGFGAHKDML
1260 1270
EGTYAEKNAD IILDILEKIT SNQVILHKR
Length:1,279
Mass (Da):144,619
Last modified:January 1, 1998 - v1
Checksum:i22EA638DEF0CEBE0
GO

Sequence cautioni

The sequence CAA74213 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13917 Genomic DNA Translation: CAA74213.1 Different initiation.
AL009126 Genomic DNA Translation: CAB13713.1
PIRiE69681
RefSeqiNP_389712.1, NC_000964.3
WP_010886522.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13713; CAB13713; BSU18300
GeneIDi939956
KEGGibsu:BSU18300
PATRICifig|224308.43.peg.1940

Similar proteinsi

Entry informationi

Entry nameiPPSE_BACSU
AccessioniPrimary (citable) accession number: O31827
Secondary accession number(s): Q799M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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