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O31801

- YNCF_BACSU

UniProt

O31801 - YNCF_BACSU

Protein

Probable deoxyuridine 5'-triphosphate nucleotidohydrolase YncF

Gene

yncF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.By similarity

    Catalytic activityi

    dUTP + H2O = dUMP + diphosphate.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei76 – 761SubstrateBy similarity

    GO - Molecular functioni

    1. dUTP diphosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dUMP biosynthetic process Source: UniProtKB-UniPathway
    2. dUTP metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU17660-MONOMER.
    UniPathwayiUPA00610; UER00666.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable deoxyuridine 5'-triphosphate nucleotidohydrolase YncF (EC:3.6.1.23)
    Short name:
    dUTPase
    Alternative name(s):
    dUTP pyrophosphatase
    Gene namesi
    Name:yncF
    Ordered Locus Names:BSU17660
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU17660.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 144144Probable deoxyuridine 5'-triphosphate nucleotidohydrolase YncFPRO_0000389001Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.By similarity

    Protein-protein interaction databases

    STRINGi224308.BSU17660.

    Structurei

    Secondary structure

    1
    144
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 119
    Beta strandi24 – 296
    Beta strandi34 – 363
    Beta strandi41 – 455
    Beta strandi48 – 514
    Beta strandi56 – 627
    Helixi66 – 705
    Beta strandi72 – 743
    Beta strandi77 – 815
    Beta strandi93 – 1008
    Beta strandi102 – 1043
    Beta strandi109 – 1179
    Beta strandi122 – 1265
    Turni138 – 1414

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XCDX-ray1.84A/B/C/D/E/F1-144[»]
    2XCEX-ray1.85A/B/C/D/E/F1-144[»]
    4AOOX-ray2.30A/B/C/D1-144[»]
    4AOZX-ray2.05A/B/C1-144[»]
    4APZX-ray2.011/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m1-144[»]
    4B0HX-ray1.18A/B/C1-144[»]
    ProteinModelPortaliO31801.
    SMRiO31801. Positions 2-130.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO31801.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 653Substrate bindingBy similarity
    Regioni80 – 823Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dUTPase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000028966.
    KOiK01520.
    OMAiIEQGDWI.
    OrthoDBiEOG689HXK.
    PhylomeDBiO31801.

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O31801-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMQIKIKYL DETQTRISKI EQGDWIDLRA AEDVTIKKDE FKLVPLGVAM    50
    ELPEGYEAHV VPRSSTYKNF GVIQTNSMGV IDESYKGDND FWFFPAYALR 100
    DTEIKKGDRI CQFRIMKKMP AVELVEVEHL GNEDRGGLGS TGTK 144
    Length:144
    Mass (Da):16,410
    Last modified:January 1, 1998 - v1
    Checksum:i2E15AE84170ED23B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB13650.1.
    PIRiH69888.
    RefSeqiNP_389649.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13650; CAB13650; BSU17660.
    GeneIDi939541.
    KEGGibsu:BSU17660.
    PATRICi18975351. VBIBacSub10457_1867.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL009126 Genomic DNA. Translation: CAB13650.1 .
    PIRi H69888.
    RefSeqi NP_389649.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XCD X-ray 1.84 A/B/C/D/E/F 1-144 [» ]
    2XCE X-ray 1.85 A/B/C/D/E/F 1-144 [» ]
    4AOO X-ray 2.30 A/B/C/D 1-144 [» ]
    4AOZ X-ray 2.05 A/B/C 1-144 [» ]
    4APZ X-ray 2.01 1/2/3/4/5/6/7/8/9/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m 1-144 [» ]
    4B0H X-ray 1.18 A/B/C 1-144 [» ]
    ProteinModelPortali O31801.
    SMRi O31801. Positions 2-130.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU17660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13650 ; CAB13650 ; BSU17660 .
    GeneIDi 939541.
    KEGGi bsu:BSU17660.
    PATRICi 18975351. VBIBacSub10457_1867.

    Organism-specific databases

    GenoListi BSU17660.

    Phylogenomic databases

    HOGENOMi HOG000028966.
    KOi K01520.
    OMAi IEQGDWI.
    OrthoDBi EOG689HXK.
    PhylomeDBi O31801.

    Enzyme and pathway databases

    UniPathwayi UPA00610 ; UER00666 .
    BioCyci BSUB:BSU17660-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O31801.

    Family and domain databases

    Gene3Di 2.70.40.10. 1 hit.
    InterProi IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51283. SSF51283. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    2. "Crystallization and preliminary X-ray analysis of three dUTPases from Gram-positive bacteria."
      Li G.-L., Wang J., Li L.-F., Su X.-D.
      Acta Crystallogr. F 65:339-342(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY CRYSTALLIZATION.

    Entry informationi

    Entry nameiYNCF_BACSU
    AccessioniPrimary (citable) accession number: O31801
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3