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Protein

Serine protease AprX

Gene

aprX

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Displays serine protease activity. Seems to have a broad substrate specificity.1 Publication

Enzyme regulationi

Is completely inhibited by phenylmethanesulphonylfluoride (PMSF) in vitro.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Charge relay systemBy similarity1
Active sitei187Charge relay systemBy similarity1
Active sitei384Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciBSUB:BSU17260-MONOMER.

Protein family/group databases

MEROPSiS08.137.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease AprX (EC:3.4.21.-)
Gene namesi
Name:aprX
Ordered Locus Names:BSU17260
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Is leaked to the culture medium during the late stationary phase owing to cell lysis.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show normal growth and sporulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003907781 – 442Serine protease AprXAdd BLAST442

Proteomic databases

PaxDbiO31788.

Expressioni

Inductioni

Is expressed during the late stationary phase of growth. Is down-regulated by SinR.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009496.

Structurei

3D structure databases

ProteinModelPortaliO31788.
SMRiO31788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 437Peptidase S8Add BLAST288

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Phylogenomic databases

eggNOGiENOG4105RX7. Bacteria.
COG1404. LUCA.
HOGENOMiHOG000199176.
InParanoidiO31788.
KOiK17734.
OMAiFNKINCC.
PhylomeDBiO31788.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O31788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGYSMVQMV RANAHKLDWP LRETVLQLYK PFKWTPCFLH KFFETKLQNR
60 70 80 90 100
KKMSVIIEFE EGCHETGFQM AGEVLQKEKR SKLKSRFNKI NCCSAEVTPS
110 120 130 140 150
ALHSLLSECS NIRKVYLNRE VKALLDTATE ASHAKEVVRN GQTLTGKGVT
160 170 180 190 200
VAVVDTGIYP HPDLEGRIIG FADMVNQKTE PYDDNGHGTH CAGDVASSGA
210 220 230 240 250
SSSGQYRGPA PEANLIGVKV LNKQGSGTLA DIIEGVEWCI QYNEDNPDEP
260 270 280 290 300
IDIMSMSLGG DALRYDHEQE DPLVRAVEEA WSAGIVVCVA AGNSGPDSQT
310 320 330 340 350
IASPGVSEKV ITVGALDDNN TASSDDDTVA SFSSRGPTVY GKEKPDILAP
360 370 380 390 400
GVNIISLRSP NSYIDKLQKS SRVGSQYFTM SGTSMATPIC AGIAALILQQ
410 420 430 440
NPDLTPDEVK ELLKNGTDKW KDEDPNIYGA GAVNAENSVP GQ
Length:442
Mass (Da):47,906
Last modified:January 1, 1998 - v1
Checksum:iB996446AE87BADF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13610.1.
PIRiA69587.
RefSeqiNP_389608.1. NC_000964.3.
WP_009967303.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13610; CAB13610; BSU17260.
GeneIDi940056.
KEGGibsu:BSU17260.
PATRICi18975261. VBIBacSub10457_1822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13610.1.
PIRiA69587.
RefSeqiNP_389608.1. NC_000964.3.
WP_009967303.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO31788.
SMRiO31788.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009496.

Protein family/group databases

MEROPSiS08.137.

Proteomic databases

PaxDbiO31788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13610; CAB13610; BSU17260.
GeneIDi940056.
KEGGibsu:BSU17260.
PATRICi18975261. VBIBacSub10457_1822.

Phylogenomic databases

eggNOGiENOG4105RX7. Bacteria.
COG1404. LUCA.
HOGENOMiHOG000199176.
InParanoidiO31788.
KOiK17734.
OMAiFNKINCC.
PhylomeDBiO31788.

Enzyme and pathway databases

BioCyciBSUB:BSU17260-MONOMER.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPRX_BACSU
AccessioniPrimary (citable) accession number: O31788
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.