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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation1 Publication

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi111Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi145Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi221Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi225Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi228Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: GO_Central
  • iron ion binding Source: UniProtKB-HAMAP
  • N6-isopentenyladenosine methylthiotransferase activity Source: UniProtKB

GO - Biological processi

  • tRNA methylthiolation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU17010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Synonyms:ymcB
Ordered Locus Names:BSU17010
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001417281 – 509tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST509

Proteomic databases

PaxDbiO31778.
PRIDEiO31778.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009356.

Structurei

3D structure databases

ProteinModelPortaliO31778.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 184MTTase N-terminalUniRule annotationAdd BLAST119
Domaini440 – 503TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
InParanoidiO31778.
KOiK06168.
OMAiCQMNFSD.
PhylomeDBiO31778.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O31778-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEKQKLESG QVHPSDKKSE KDYSKYFEAV YIPPSLKDAK KRGKEAVTYH
60 70 80 90 100
NDFKISEQFK GLGDGRKFYI RTYGCQMNEH DTEVMAGIFM ALGYEATNSV
110 120 130 140 150
DDANVILLNT CAIRENAENK VFGELGHLKA LKKNNPDLIL GVCGCMSQEE
160 170 180 190 200
SVVNRILKKH PFVDMIFGTH NIHRLPELLS EAYLSKEMVV EVWSKEGDVI
210 220 230 240 250
ENLPKVRNGK IKGWVNIMYG CDKFCTYCIV PYTRGKERSR RPEDIIQEVR
260 270 280 290 300
RLASEGYKEI TLLGQNVNAY GKDFEDMTYG LGDLMDELRK IDIPRIRFTT
310 320 330 340 350
SHPRDFDDRL IEVLAKGGNL LDHIHLPVQS GSSEVLKLMA RKYDRERYME
360 370 380 390 400
LVRKIKEAMP NASLTTDIIV GFPNETDEQF EETLSLYREV EFDSAYTFIY
410 420 430 440 450
SPREGTPAAK MKDNVPMRVK KERLQRLNAL VNEISAKKMK EYEGKVVEVL
460 470 480 490 500
VEGESKNNPD ILAGYTEKSK LVNFKGPKEA IGKIVRVKIQ QAKTWSLDGE

MVGEAIEVK
Length:509
Mass (Da):58,170
Last modified:January 1, 1998 - v1
Checksum:i163BBCE3025F196E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13574.1.
PIRiD69884.
RefSeqiNP_389583.1. NC_000964.3.
WP_003244831.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13574; CAB13574; BSU17010.
GeneIDi939451.
KEGGibsu:BSU17010.
PATRICi18975209. VBIBacSub10457_1796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13574.1.
PIRiD69884.
RefSeqiNP_389583.1. NC_000964.3.
WP_003244831.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO31778.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009356.

Proteomic databases

PaxDbiO31778.
PRIDEiO31778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13574; CAB13574; BSU17010.
GeneIDi939451.
KEGGibsu:BSU17010.
PATRICi18975209. VBIBacSub10457_1796.

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
InParanoidiO31778.
KOiK06168.
OMAiCQMNFSD.
PhylomeDBiO31778.

Enzyme and pathway databases

BioCyciBSUB:BSU17010-MONOMER.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIAB_BACSU
AccessioniPrimary (citable) accession number: O31778
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.