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Protein

Proline--tRNA ligase

Gene

proS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU16570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:BSU16570
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Proline--tRNA ligasePRO_0000139320Add
BLAST

Proteomic databases

PaxDbiO31755.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiO31755. 1 interaction.
MINTiMINT-8365685.
STRINGi224308.Bsubs1_010100009131.

Structurei

3D structure databases

ProteinModelPortaliO31755.
SMRiO31755. Positions 1-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C90. Bacteria.
COG0442. LUCA.
HOGENOMiHOG000076893.
InParanoidiO31755.
KOiK01881.
OMAiIQPAELW.
PhylomeDBiO31755.

Family and domain databases

CDDicd00779. ProRS_core_prok. 1 hit.
Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR033730. ProRS_core_prok.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PIRSFiPIRSF001535. ProRS_1. 1 hit.
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O31755-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQSLTLIPT LREVPADAEA KSHQLLLRAG FIRQNTSGVY SYMPLAYKVI
60 70 80 90 100
QNIQQIVREE MEKIDAVEML MPALQQAETW QESGRWYTYG PELMRLKDRH
110 120 130 140 150
GREFALGATH EEVITSLVRD EVKSYKRLPL TLYQIQSKFR DEKRPRFGLL
160 170 180 190 200
RGREFIMKDA YSFHASAESL DETYQKMYEA YSNIFARCGI NVRPVIADSG
210 220 230 240 250
AMGGKDTHEF MALSAIGEDT IAYSDESQYA ANIEMAEVLH QEVPSDEEPK
260 270 280 290 300
ALEKVHTPNV KTIEELTAFL QVSAEACIKS VLFKADDRFV LVLVRGDHEV
310 320 330 340 350
NDIKVKNLLH AEVVELATHE EVIQQLGTEP GFVGPVGIHQ DVEVYADQAV
360 370 380 390 400
KAMVNAVAGA NEGDHHYKNV NVNRDAQIKE FADLRFIKEG DPSPDGKGTI
410 420 430 440 450
RFAEGIEVGQ VFKLGTRYSE AMNATYLDEN GRAQPMLMGC YGIGVSRTLS
460 470 480 490 500
AIAEQHHDEK GLIWPKSVAP YDLHILALNM KNDGQRELAE KLYADLKAEG
510 520 530 540 550
YEVLYDDRAE RAGVKFADSD LIGLPIRITV GKRADEGIVE VKIRQTGEST
560
EISVDELSAF ISKQ
Length:564
Mass (Da):63,305
Last modified:January 1, 1998 - v1
Checksum:iC8068EE6072C4F07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13530.1.
PIRiG69682.
RefSeqiNP_389539.1. NC_000964.3.
WP_003231918.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13530; CAB13530; BSU16570.
GeneIDi939617.
KEGGibsu:BSU16570.
PATRICi18975121. VBIBacSub10457_1752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13530.1.
PIRiG69682.
RefSeqiNP_389539.1. NC_000964.3.
WP_003231918.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO31755.
SMRiO31755. Positions 1-563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO31755. 1 interaction.
MINTiMINT-8365685.
STRINGi224308.Bsubs1_010100009131.

Proteomic databases

PaxDbiO31755.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13530; CAB13530; BSU16570.
GeneIDi939617.
KEGGibsu:BSU16570.
PATRICi18975121. VBIBacSub10457_1752.

Phylogenomic databases

eggNOGiENOG4105C90. Bacteria.
COG0442. LUCA.
HOGENOMiHOG000076893.
InParanoidiO31755.
KOiK01881.
OMAiIQPAELW.
PhylomeDBiO31755.

Enzyme and pathway databases

BioCyciBSUB:BSU16570-MONOMER.

Family and domain databases

CDDicd00779. ProRS_core_prok. 1 hit.
Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR033730. ProRS_core_prok.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PIRSFiPIRSF001535. ProRS_1. 1 hit.
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYP_BACSU
AccessioniPrimary (citable) accession number: O31755
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.