Reviewed, UniProtKB/Swiss-Prot O31678 (QUEF_BACSU)

Names and originHide

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
PreQ(0) reductase
NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Synonyms:	ykvM
Ordered Locus Names:	BSU13750

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributesHide

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)Hide

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). Ref.3
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subunit structure	Homododecamer. Ref.3
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

OntologiesHide

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000162957

SequencesHide

	Sequence		Length	Mass (Da)
	O31678-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E1CEF14D1EAA007D	FASTA	165	19,375
	10 20 30 40 50 60 MTTRKESELE GVTLLGNQGT NYLFEYAPDV LESFPNKHVN RDYFVKFNCP EFTSLCPKTG 70 80 90 100 110 120 QPDFATIYIS YIPDEKMVES KSLKLYLFSF RNHGDFHEDC MNIIMNDLIE LMDPRYIEVW 130 140 150 160 GKFTPRGGIS IDPYTNYGKP GTKYEKMAEY RMMNHDLYPE TIDNR

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine." Reader J.S., Metzgar D., Schimmel P., de Crecy-Lagard V. J. Biol. Chem. 279:6280-6285(2004) [PubMed: 14660578] [Abstract] Cited for: INVOLVEMENT IN QUEUOSINE BIOSYNTHESIS, GENE NAME.
[3]	"From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold." Van Lanen S.G., Reader J.S., Swairjo M.A., de Crecy-Lagard V., Lee B., Iwata-Reuyl D. Proc. Natl. Acad. Sci. U.S.A. 102:4264-4269(2005) [PubMed: 15767583] [Abstract] Cited for: FUNCTION, SUBUNIT.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	AL009126 Genomic DNA. Translation: CAB13248.1.
PIR	D69868.
RefSeq	NP_389258.1.
3D structure databases
ModBase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001500; EBBACP00000001500; EBBACG00000001498.
GeneID	939296.
GenomeReviews	Gene locus BSU13750 in contig AL009126_GR.
KEGG	bsu:BSU13750.
NMPDR	fig|224308.1.peg.1377.
Organism-specific databases
GenoList	BSU13750. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG294103.
OMA	CINTILL.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	SUBTI:BSU13750-MONOMER.
BRENDA	1.7.1.13. 150.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry informationHide

Entry name

QUEF_BACSU

Accession

Primary (citable) accession number: O31678

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	January 1, 1998
Last modified:	July 13, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)