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Protein

7-cyano-7-deazaguanine synthase

Gene

queC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ0). Uses ammonia as nitrogen donor.2 Publications

Catalytic activityi

7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O.1 Publication

Cofactori

Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.2 Publications

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi186Zinc1 Publication1
Metal bindingi195Zinc1 Publication1
Metal bindingi198Zinc1 Publication1
Metal bindingi201Zinc1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 20ATP1 PublicationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU13720-MONOMER.
MetaCyc:BSU13720-MONOMER.
BRENDAi6.3.4.20. 658.
UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
7-cyano-7-deazaguanine synthase (EC:6.3.4.201 Publication)
Alternative name(s):
7-cyano-7-carbaguanine synthase
PreQ(0) synthase
Queuosine biosynthesis protein QueC
Gene namesi
Name:queC
Synonyms:ykvJ
Ordered Locus Names:BSU13720
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002468011 – 2197-cyano-7-deazaguanine synthaseAdd BLAST219

Proteomic databases

PaxDbiO31675.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007596.

Structurei

Secondary structure

1219
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi14 – 26Combined sources13
Beta strandi28 – 38Combined sources11
Helixi43 – 53Combined sources11
Beta strandi59 – 63Combined sources5
Helixi65 – 70Combined sources6
Helixi73 – 75Combined sources3
Helixi97 – 112Combined sources16
Beta strandi115 – 118Combined sources4
Helixi130 – 132Combined sources3
Helixi134 – 148Combined sources15
Beta strandi153 – 155Combined sources3
Turni157 – 160Combined sources4
Helixi163 – 172Combined sources10
Helixi176 – 182Combined sources7
Helixi199 – 213Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BL5X-ray2.95A/B/C/D/E/F1-219[»]
ProteinModelPortaliO31675.
SMRiO31675.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO31675.

Family & Domainsi

Sequence similaritiesi

Belongs to the QueC family.Curated

Phylogenomic databases

eggNOGiENOG4105FD2. Bacteria.
COG0603. LUCA.
HOGENOMiHOG000110563.
InParanoidiO31675.
KOiK06920.
OMAiAYGYGCD.
PhylomeDBiO31675.

Family and domain databases

CDDicd01995. ExsB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01633. QueC. 1 hit.
InterProiIPR018317. QueC.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR11587:SF3. PTHR11587:SF3. 1 hit.
PfamiPF06508. QueC. 1 hit.
[Graphical view]
PIRSFiPIRSF006293. ExsB. 1 hit.
TIGRFAMsiTIGR00364. TIGR00364. 1 hit.

Sequencei

Sequence statusi: Complete.

O31675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKEKAIVVF SGGQDSTTCL LWALKEFEEV ETVTFHYNQR HSQEVEVAKS
60 70 80 90 100
IAEKLGVKNH LLDMSLLNQL APNALTRNDI EIEVKDGELP STFVPGRNLV
110 120 130 140 150
FLSFASILAY QIGARHIITG VCETDFSGYP DCRDEFVKSC NVTVNLAMEK
160 170 180 190 200
PFVIHTPLMW LNKAETWKLA DELGALDFVK NNTLTCYNGI IADGCGECPA
210
CHLRSKGYEE YMVMKGERA
Length:219
Mass (Da):24,529
Last modified:January 1, 1998 - v1
Checksum:i146535A4A91EB894
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13245.1.
PIRiA69868.
RefSeqiNP_389255.1. NC_000964.3.
WP_003245417.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13245; CAB13245; BSU13720.
GeneIDi939292.
KEGGibsu:BSU13720.
PATRICi18974513. VBIBacSub10457_1448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13245.1.
PIRiA69868.
RefSeqiNP_389255.1. NC_000964.3.
WP_003245417.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BL5X-ray2.95A/B/C/D/E/F1-219[»]
ProteinModelPortaliO31675.
SMRiO31675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007596.

Proteomic databases

PaxDbiO31675.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13245; CAB13245; BSU13720.
GeneIDi939292.
KEGGibsu:BSU13720.
PATRICi18974513. VBIBacSub10457_1448.

Phylogenomic databases

eggNOGiENOG4105FD2. Bacteria.
COG0603. LUCA.
HOGENOMiHOG000110563.
InParanoidiO31675.
KOiK06920.
OMAiAYGYGCD.
PhylomeDBiO31675.

Enzyme and pathway databases

UniPathwayiUPA00391.
BioCyciBSUB:BSU13720-MONOMER.
MetaCyc:BSU13720-MONOMER.
BRENDAi6.3.4.20. 658.

Miscellaneous databases

EvolutionaryTraceiO31675.

Family and domain databases

CDDicd01995. ExsB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01633. QueC. 1 hit.
InterProiIPR018317. QueC.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR11587:SF3. PTHR11587:SF3. 1 hit.
PfamiPF06508. QueC. 1 hit.
[Graphical view]
PIRSFiPIRSF006293. ExsB. 1 hit.
TIGRFAMsiTIGR00364. TIGR00364. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEC_BACSU
AccessioniPrimary (citable) accession number: O31675
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.