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Protein

ATP-dependent Clp protease ATP-binding subunit ClpE

Gene

clpE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus a role in overall protein quality control in response to heat stress.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3230C4-typeAdd
BLAST
Nucleotide bindingi119 – 1268ATPSequence analysis
Nucleotide bindingi446 – 4538ATPSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU13700-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease ATP-binding subunit ClpE
Alternative name(s):
ATPase ClpE
Heat shock protein HSP1
Gene namesi
Name:clpE
Ordered Locus Names:BSU13700
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene display a retardation of CtsR-dependent gene induction as well as a delayed restoration of the repressed stage. They also show a significant delay in disaggregation of heat-generated insoluble protein aggregates.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi29 – 324CETC → SETS: 10% of wild-type ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 699699ATP-dependent Clp protease ATP-binding subunit ClpEPRO_0000390779Add
BLAST

Post-translational modificationi

ClpE is a very short-lived protein, that is, at least in the soluble cell fraction, degraded mainly by ClpCP.

Proteomic databases

PaxDbiO31673.
PRIDEiO31673.

Expressioni

Inductioni

By heat shock via the relief of repression carried out by the transcriptional regulator CtsR.2 Publications

Interactioni

Subunit structurei

Interacts with ClpP.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007586.

Structurei

3D structure databases

ProteinModelPortaliO31673.
SMRiO31673. Positions 65-698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini325 – 36036UVRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ClpA/ClpB family. ClpE subfamily.Curated
Contains 1 UVR domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3230C4-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0542. LUCA.
HOGENOMiHOG000218210.
InParanoidiO31673.
KOiK03697.
OMAiEWVEDEM.
PhylomeDBiO31673.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
PF02151. UVR. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O31673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCQHCHQNE ATIRLNMQIN SVHKQMVLCE TCYNELTRKP SMSMGPQSFG
60 70 80 90 100
FPFEQAFQPK EQSAAKQSEK KGLLDELAQN ITNGAKAGLI DPVIGRDDEV
110 120 130 140 150
ARVIEILNRR NKNNPVLIGE PGVGKTAIAE GLALKIAEGD VPNKLKNKEL
160 170 180 190 200
YLLDVASLVA NTGIRGQFEE RMKQLITELK ERKNVILFID EIHLLVGAGS
210 220 230 240 250
AEGSMDAGNI LKPALARGEL QVIGATTLKE YRQIEKDAAL ERRFQPVMVQ
260 270 280 290 300
EPSIEQAILI LQGIKDKYEA YHGVTFSDEA IKACVTLSSR YIQDRHLPDK
310 320 330 340 350
AIDLLDEAGS KANLLIDELN DEDAAERLTA IEAEKTKALE EENYELAAKL
360 370 380 390 400
RDEELALEKK LNSSSAHTAV TVEAEHIQEI VEQKTGIPVG KLQADEQTKM
410 420 430 440 450
KELEAKLHER VIGQEAAVQK VAKAVRRSRA GLKSKNRPVG SFLFVGPTGV
460 470 480 490 500
GKTELSKTLA DELFGTKDAI IRLDMSEYME KHAVSKIIGS PPGYVGHEEA
510 520 530 540 550
GQLTEKVRRN PYSIVLLDEI EKAHPDVQHM FLQIMEDGRL TDSQGRTVSF
560 570 580 590 600
KDTVIIMTSN AGAGEKQTKV GFQSDDSVIE EQTLIDSLSM FFKPEFLNRF
610 620 630 640 650
DSIIEFRSLE KEHLVKIVSL LLGELEETLA ERGISLNVTD EAKEKIAELG
660 670 680 690
YHPSFGARPL RRTIQEWVED EMTDLLLDNG EITSFHVILE DDKIKVRAK
Length:699
Mass (Da):77,906
Last modified:January 1, 1998 - v1
Checksum:i5F45A749CF42A0AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13243.1.
PIRiA69601.
RefSeqiNP_389253.1. NC_000964.3.
WP_009967104.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13243; CAB13243; BSU13700.
GeneIDi939289.
KEGGibsu:BSU13700.
PATRICi18974509. VBIBacSub10457_1446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13243.1.
PIRiA69601.
RefSeqiNP_389253.1. NC_000964.3.
WP_009967104.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO31673.
SMRiO31673. Positions 65-698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007586.

Proteomic databases

PaxDbiO31673.
PRIDEiO31673.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13243; CAB13243; BSU13700.
GeneIDi939289.
KEGGibsu:BSU13700.
PATRICi18974509. VBIBacSub10457_1446.

Phylogenomic databases

eggNOGiCOG0542. LUCA.
HOGENOMiHOG000218210.
InParanoidiO31673.
KOiK03697.
OMAiEWVEDEM.
PhylomeDBiO31673.

Enzyme and pathway databases

BioCyciBSUB:BSU13700-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
PF02151. UVR. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPE_BACSU
AccessioniPrimary (citable) accession number: O31673
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.