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Protein

Sporulation kinase D

Gene

kinD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates the sporulation-regulatory protein spo0F and, to a minor extent, is responsible for heterogeneous expression of spo0A during logarithmical growth. Also phosphorylates spo0A under biofilm growth conditions.3 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processSporulation, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU13660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation kinase D (EC:2.7.13.3)
Alternative name(s):
Sensor histidine kinase D
Gene namesi
Name:kinD
Synonyms:ykvD
Ordered Locus Names:BSU13660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Transmembranei250 – 270HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene sporulate normally in spo0F mutants.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003752631 – 506Sporulation kinase DAdd BLAST506

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei301Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO31671.

Expressioni

Inductioni

Expressed during growth and early stationary phase.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007566.

Structurei

Secondary structure

1506
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 79Combined sources40
Helixi86 – 97Combined sources12
Beta strandi103 – 110Combined sources8
Beta strandi114 – 120Combined sources7
Helixi132 – 140Combined sources9
Turni152 – 154Combined sources3
Beta strandi156 – 166Combined sources11
Beta strandi172 – 181Combined sources10
Helixi182 – 192Combined sources11
Beta strandi198 – 202Combined sources5
Beta strandi207 – 212Combined sources6
Helixi214 – 217Combined sources4
Beta strandi223 – 226Combined sources4
Beta strandi228 – 230Combined sources3
Beta strandi232 – 236Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JGOX-ray2.28A/B37-250[»]
4JGPX-ray2.03A/B37-250[»]
4JGQX-ray2.63A/B37-250[»]
4JGRX-ray2.40A/B37-250[»]
ProteinModelPortaliO31671.
SMRiO31671.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO31671.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini298 – 505Histidine kinasePROSITE-ProRule annotationAdd BLAST208

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000086165.
InParanoidiO31671.
KOiK13532.
OMAiYNGHIFI.
PhylomeDBiO31671.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR033479. dCache_1.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
PfamiView protein in Pfam
PF02743. dCache_1. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiView protein in PROSITE
PS50109. HIS_KIN. 1 hit.

Sequencei

Sequence statusi: Complete.

O31671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLERCKLKIL KGACGRVKLY IILVVIPAIV ISFFVYEKEK DTIAAEHKQE
60 70 80 90 100
ASVLLNLHRN KINYLIGETM ARMTSLSIAI DRPVDIKKMQ SILEKTFDSE
110 120 130 140 150
PRFSGLYFLN AKGDVTASTT ELKTKVNLAD RSFFIKAKET KKTVISDSYS
160 170 180 190 200
SRITGQPIFT ICVPVLDSKR NVTDYLVAAI QIDYLKNLIN LLSPDVYIEV
210 220 230 240 250
VNQDGKMIFA SGQASHAEDQ KPVSGYLDDI SWNMKVYPNP VTIEELSKSL
260 270 280 290 300
VLPLSCIIVL LNILFILVLY YLLKRQTQLE RSENEAQKLE LIGTLAASTA
310 320 330 340 350
HEIRNPLTGI SGFIQLLQKK YKGEEDQLYF SIIEQEIKRI NQIVSEFLVL
360 370 380 390 400
GKPTAEKWEL NSLQDIIGEI MPIIYSEGNL YNVEVELQYL TEQPLLVKCT
410 420 430 440 450
KDHIKQVILN VAKNGLESMP EGGKLTISLG ALDKKAIIKV VDNGEGISQE
460 470 480 490 500
MLDHIFLPFV TSKEKGTGLG LVVCKRIVLM YGGSIHIESE VRRGTEVTIT

LPVSAS
Length:506
Mass (Da):56,704
Last modified:January 1, 1998 - v1
Checksum:i4A93A6A4500C179A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13239.1.
PIRiF69867.
RefSeqiNP_389249.1. NC_000964.3.
WP_010886497.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13239; CAB13239; BSU13660.
GeneIDi939300.
KEGGibsu:BSU13660.
PATRICifig|224308.43.peg.1442.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiKIND_BACSU
AccessioniPrimary (citable) accession number: O31671
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: January 1, 1998
Last modified: July 5, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references