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Protein

Acireductone dioxygenase

Gene

mtnD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.1 Publication

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)propanoate + formate + CO.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationCuratedNote: Binds 1 Fe cation per monomer.Curated
  • Ni2+CuratedNote: Binds 1 nickel ion per monomer.Curated

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (mtnA)
  2. Methylthioribulose-1-phosphate dehydratase (mtnB)
  3. 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW)
  4. 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX)
  5. Acireductone dioxygenase (mtnD)
  6. Transaminase MtnE (mtnE)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei99May play a role in metal incorporation in vivoBy similarity1
Metal bindingi100Iron; alternateBy similarity1
Metal bindingi100Nickel; alternateBy similarity1
Metal bindingi102Iron; alternateBy similarity1
Metal bindingi102Nickel; alternateBy similarity1
Sitei105May play a role in transmitting local conformational changesBy similarity1
Metal bindingi106Iron; alternateBy similarity1
Metal bindingi106Nickel; alternateBy similarity1
Sitei108Important to generate the dianionBy similarity1
Metal bindingi145Iron; alternateBy similarity1
Metal bindingi145Nickel; alternateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Nickel

Enzyme and pathway databases

BioCyciBSUB:BSU13620-MONOMER.
UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
Acireductone dioxygenase
Alternative name(s):
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Short name:
DHK-MTPene dioxygenase
Acireductone dioxygenase (Fe(2+)-requiring) (EC:1.13.11.54)
Short name:
ARD'
Short name:
Fe-ARD
Acireductone dioxygenase (Ni(2+)-requiring) (EC:1.13.11.53)
Short name:
ARD
Short name:
Ni-ARD
Gene namesi
Name:mtnD
Synonyms:ykrZ
Ordered Locus Names:BSU13620
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001629411 – 178Acireductone dioxygenaseAdd BLAST178

Proteomic databases

PaxDbiO31669.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiO31669. 1 interactor.
MINTiMINT-8366236.
STRINGi224308.Bsubs1_010100007546.

Structurei

3D structure databases

ProteinModelPortaliO31669.
SMRiO31669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108CAF. Bacteria.
COG1791. LUCA.
HOGENOMiHOG000201072.
InParanoidiO31669.
KOiK08967.
OMAiCHTHADD.
PhylomeDBiO31669.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01682. Salvage_MtnD. 1 hit.
InterProiIPR004313. ARD.
IPR023956. ARD_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

O31669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIRIHDEA NTTIENQEEV ASFLDSQEVI YEQWDITRLP EHLSEKYDLT
60 70 80 90 100
EEEKQQILDT FETEIKDIST RRGYKAQDVI SLSDSNPKLD ELLENFKREH
110 120 130 140 150
HHTDDEVRFI VSGHGIFVIQ GQDGTFFDVR LNPGDLISVP ENIRHYFTLQ
160 170
EDRKVVAVRI FVTTEGWVPI YEKDSVNQ
Length:178
Mass (Da):20,824
Last modified:January 1, 1998 - v1
Checksum:i4E29680F99D33594
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13235.1.
PIRiB69864.
RefSeqiNP_389245.1. NC_000964.3.
WP_003244952.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13235; CAB13235; BSU13620.
GeneIDi939322.
KEGGibsu:BSU13620.
PATRICi18974493. VBIBacSub10457_1438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13235.1.
PIRiB69864.
RefSeqiNP_389245.1. NC_000964.3.
WP_003244952.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO31669.
SMRiO31669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO31669. 1 interactor.
MINTiMINT-8366236.
STRINGi224308.Bsubs1_010100007546.

Proteomic databases

PaxDbiO31669.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13235; CAB13235; BSU13620.
GeneIDi939322.
KEGGibsu:BSU13620.
PATRICi18974493. VBIBacSub10457_1438.

Phylogenomic databases

eggNOGiENOG4108CAF. Bacteria.
COG1791. LUCA.
HOGENOMiHOG000201072.
InParanoidiO31669.
KOiK08967.
OMAiCHTHADD.
PhylomeDBiO31669.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878.
BioCyciBSUB:BSU13620-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01682. Salvage_MtnD. 1 hit.
InterProiIPR004313. ARD.
IPR023956. ARD_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTND_BACSU
AccessioniPrimary (citable) accession number: O31669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.