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Protein

Acireductone dioxygenase

Gene

mtnD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.1 Publication

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)propanoate + formate + CO.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationCuratedNote: Binds 1 Fe cation per monomer.Curated
  • Ni2+CuratedNote: Binds 1 nickel ion per monomer.Curated

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (mtnA)
  2. Methylthioribulose-1-phosphate dehydratase (mtnB)
  3. 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW)
  4. 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX)
  5. Acireductone dioxygenase (mtnD)
  6. Transaminase MtnE (mtnE)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei99May play a role in metal incorporation in vivoBy similarity1
Metal bindingi100Iron; alternateBy similarity1
Metal bindingi100Nickel; alternateBy similarity1
Metal bindingi102Iron; alternateBy similarity1
Metal bindingi102Nickel; alternateBy similarity1
Sitei105May play a role in transmitting local conformational changesBy similarity1
Metal bindingi106Iron; alternateBy similarity1
Metal bindingi106Nickel; alternateBy similarity1
Sitei108Important to generate the dianionBy similarity1
Metal bindingi145Iron; alternateBy similarity1
Metal bindingi145Nickel; alternateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandIron, Metal-binding, Nickel

Enzyme and pathway databases

BioCyciBSUB:BSU13620-MONOMER
UniPathwayiUPA00904; UER00878

Names & Taxonomyi

Protein namesi
Recommended name:
Acireductone dioxygenase
Alternative name(s):
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Short name:
DHK-MTPene dioxygenase
Acireductone dioxygenase (Fe(2+)-requiring) (EC:1.13.11.54)
Short name:
ARD'
Short name:
Fe-ARD
Acireductone dioxygenase (Ni(2+)-requiring) (EC:1.13.11.53)
Short name:
ARD
Short name:
Ni-ARD
Gene namesi
Name:mtnD
Synonyms:ykrZ
Ordered Locus Names:BSU13620
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001629411 – 178Acireductone dioxygenaseAdd BLAST178

Proteomic databases

PaxDbiO31669
PRIDEiO31669

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiO31669, 1 interactor
MINTiO31669
STRINGi224308.Bsubs1_010100007546

Structurei

3D structure databases

ProteinModelPortaliO31669
SMRiO31669
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108CAF Bacteria
COG1791 LUCA
HOGENOMiHOG000201072
InParanoidiO31669
KOiK08967
OMAiWFDMGSE
PhylomeDBiO31669

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_01682 Salvage_MtnD, 1 hit
InterProiView protein in InterPro
IPR004313 ARD
IPR023956 ARD_bac
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR23418 PTHR23418, 1 hit
PfamiView protein in Pfam
PF03079 ARD, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit

Sequencei

Sequence statusi: Complete.

O31669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIRIHDEA NTTIENQEEV ASFLDSQEVI YEQWDITRLP EHLSEKYDLT
60 70 80 90 100
EEEKQQILDT FETEIKDIST RRGYKAQDVI SLSDSNPKLD ELLENFKREH
110 120 130 140 150
HHTDDEVRFI VSGHGIFVIQ GQDGTFFDVR LNPGDLISVP ENIRHYFTLQ
160 170
EDRKVVAVRI FVTTEGWVPI YEKDSVNQ
Length:178
Mass (Da):20,824
Last modified:January 1, 1998 - v1
Checksum:i4E29680F99D33594
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13235.1
PIRiB69864
RefSeqiNP_389245.1, NC_000964.3
WP_003244952.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13235; CAB13235; BSU13620
GeneIDi939322
KEGGibsu:BSU13620
PATRICifig|224308.179.peg.1479

Similar proteinsi

Entry informationi

Entry nameiMTND_BACSU
AccessioniPrimary (citable) accession number: O31669
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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