O31664 (MTNU_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UPF0012 hydrolase mtnU
EC=3.5.-.-

Gene names

Name:	mtnU
Synonyms:	ykrU
Ordered Locus Names:	BSU13570

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in a regulatory step in the methylthioribose (MTR) recycling pathway. Ref.2
Disruption phenotype	Impaired growth on methylthioribose (MTR) as sole sulfur source. Ref.2
Sequence similarities	Belongs to the UPF0012 family. Contains 1 CN hydrolase domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	UPF0012 hydrolase mtnU		PRO_0000360754
Regions
	Domain	3 – 259	257	CN hydrolase
Sites
	Active site	42	1	Proton acceptor Potential
	Active site	111	1	By similarity
	Active site	145	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31664 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 89A2233252CCBCFA	FASTA	259	29,399
	10 20 30 40 50 60 MKWTISCLQF DISYGKPSEN IKKAEFFIEK ESKHADVLVL PELWTTGYDL ANLDELADED 70 80 90 100 110 120 GRSAQSWLKK TAKKHGVHIV AGSVAVRKNS DVYNTMYIAD KEGQIIKEYR KAHLFQLMDE 130 140 150 160 170 180 HLYLSAGSED GYFELDGVKS SGLICYDIRF PEWIRKHTTK GANVLFISAE WPLPRLDHWK 190 200 210 220 230 240 SLLIARAIEN QCFVAACNCT GSNPDNEFAG HSLIIDPWGR VLAEGGREEG IVRAEIDLQE 250 SAEVRESIPV FDDIRKDLY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"The methionine salvage pathway in Bacillus subtilis." Sekowska A., Danchin A. BMC Microbiol. 2:8-8(2002) [PubMed: 12022921] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL009126 Genomic DNA. Translation: CAB13230.1.
PIR	E69863.
RefSeq	NP_389240.1. NC_000964.3.
3D structure databases
HSSP	HSSP built from PDB template 1EMS based on UniProtKB O76463.
ProteinModelPortal	O31664.
SMR	O31664. Positions 2-259.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003066; EBBACP00000003066; EBBACG00000003060.
GeneID	939328.
GenomeReviews	Gene locus BSU13570 in contig AL009126_GR.
KEGG	bsu:BSU13570.
NMPDR	fig|224308.1.peg.1359.
PATRIC	18974482. VBIBacSub10457_1433.
Organism-specific databases
GenoList	BSU13570. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001845.
HOGENOM	HBG742418.
OMA	FISAEWP.
PhylomeDB	O31664.
ProtClustDB	CLSK2393381.
Enzyme and pathway databases
BioCyc	BSUB:BSU13570-MONOMER.
Family and domain databases
InterPro	IPR003010. Ntlse/CNhydtse. [Graphical view]
Gene3D	G3DSA:3.60.110.10. Ntlse/CNhydtse. 1 hit.
KO	K08590.
Pfam	PF00795. CN_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56317. Ntlse/CNhydtse. 1 hit.
PROSITE	PS50263. CN_HYDROLASE. 1 hit. PS01227. UPF0012. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTNU_BACSU

Accession

Primary (citable) accession number: O31664

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program