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Protein

Sporulation kinase E

Gene

kinE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Sporulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU13530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation kinase E (EC:2.7.13.3)
Alternative name(s):
Sensor histidine kinase E
Gene namesi
Name:kinE
Synonyms:ykrQ
Ordered Locus Names:BSU13530
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003752641 – 738Sporulation kinase EAdd BLAST738

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei526Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO31661.

Expressioni

Inductioni

Expressed during growth and early stationary phase.1 Publication

Interactioni

Protein-protein interaction databases

IntActiO31661. 1 interactor.
STRINGi224308.Bsubs1_010100007496.

Structurei

3D structure databases

ProteinModelPortaliO31661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 99PAS 1PROSITE-ProRule annotationAdd BLAST71
Domaini150 – 220PAS 2PROSITE-ProRule annotationAdd BLAST71
Domaini271 – 342PAS 3PROSITE-ProRule annotationAdd BLAST72
Domaini391 – 462PAS 4PROSITE-ProRule annotationAdd BLAST72
Domaini523 – 729Histidine kinasePROSITE-ProRule annotationAdd BLAST207

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 4 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG2202. LUCA.
HOGENOMiHOG000261831.
InParanoidiO31661.
KOiK13533.
OMAiCKSFEIG.
PhylomeDBiO31661.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
PF13188. PAS_8. 2 hits.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 4 hits.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 4 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 4 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O31661-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLGVQTNS ELREELNRLK EENARLKKEL NQHQVIVNNT LDAIFICDNE
60 70 80 90 100
MRIVQANEAT ERMLQVDSED LKKRSVLDFL FSIPKDELNL SVKKFFKKGF
110 120 130 140 150
LWKEVPIRLD CGATKYIEFL AKRGIGEDFF FVVMRDISSK KILEREFSMN
160 170 180 190 200
EQLFKDLFDR AVDGIVLFDK DGGFIDANLS FCKSFEINHN ELSHLSLYEF
210 220 230 240 250
IDSGSRKDFD NIWKALNRKG KAKGELPVKL RSGVQKLFEF TITSNIISGF
260 270 280 290 300
YMSIMRDITE KRSMELQLFK SEERFREIFE NAMDAIIIWS NDGRIVKANQ
310 320 330 340 350
SACKIFELPM NLLLKRKLCD FLVDSQQKYS ITKRKYAKYG EIREELLFQM
360 370 380 390 400
GNGQFKELEF TSKRTILENQ HLTILRNVSD RKRMEKELRE SELKFRKVFN
410 420 430 440 450
GSMDGNVLFD NQYRIIDANP LASHILGLSH EEIKQHSLLD IISAYEIENL
460 470 480 490 500
ASPARQINFD EMDNEIPFLL SSGDNRKLEF SFKRNIIQNM NLAIFKDVTE
510 520 530 540 550
RKELEERLRK SDTLHVVGEL AAGIAHEIRN PMTALKGFIQ LLKGSVEGDY
560 570 580 590 600
ALYFNVITSE LKRIESIITE FLILAKPQAI MYEEKHVTQI MRDTIDLLNA
610 620 630 640 650
QANLSNVQMQ LDLIDDIPPI YCEPNQLKQV FINILKNAIE VMPDGGNIFV
660 670 680 690 700
TIKALDQDHV LISLKDEGIG MTEDKLKRLG EPFYTTKERG TGLGLMVSYK
710 720 730
IIEEHQGEIM VESEEGKGTV FHITLPVRQN AEERRNDE
Length:738
Mass (Da):85,513
Last modified:January 1, 1998 - v1
Checksum:iFE27DAB7F08AC64B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13226.1.
PIRiB69863.
RefSeqiNP_389236.1. NC_000964.3.
WP_003232504.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13226; CAB13226; BSU13530.
GeneIDi939349.
KEGGibsu:BSU13530.
PATRICi18974472. VBIBacSub10457_1428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13226.1.
PIRiB69863.
RefSeqiNP_389236.1. NC_000964.3.
WP_003232504.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliO31661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO31661. 1 interactor.
STRINGi224308.Bsubs1_010100007496.

Proteomic databases

PaxDbiO31661.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13226; CAB13226; BSU13530.
GeneIDi939349.
KEGGibsu:BSU13530.
PATRICi18974472. VBIBacSub10457_1428.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG2202. LUCA.
HOGENOMiHOG000261831.
InParanoidiO31661.
KOiK13533.
OMAiCKSFEIG.
PhylomeDBiO31661.

Enzyme and pathway databases

BioCyciBSUB:BSU13530-MONOMER.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
PF13188. PAS_8. 2 hits.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 4 hits.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 4 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 4 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKINE_BACSU
AccessioniPrimary (citable) accession number: O31661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.