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Reviewed, UniProtKB/Swiss-Prot O31646 (MANA1_BACSU)

Last modified November 3, 2009. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Mannose-6-phosphate isomerase manA
    EC=5.3.1.8
Alternative name(s):
    Phosphomannose isomerase
      Short name=PMI
    Phosphohexomutase
Gene names
Name: manA
Synonyms: pmi, yjdE
Ordered Locus Names: BSU12020
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Disruption phenotype

Cells lacking this gene are unable to grow in minimal medium with mannose as a sole carbon source. They show impaired growth in rich medium, and impairment increases in the presence of greater than 1.4 mM mannose. Ref.3

Sequence similarities

Belongs to the mannose-6-phosphate isomerase type 1 family.

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Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionmannose-6-phosphate isomerase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Mannose-6-phosphate isomerase manA
PRO_0000371314

Sites

Active site1921 By similarity
Metal binding971Zinc By similarity
Metal binding1101Zinc By similarity
Metal binding1721Zinc By similarity

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Sequences

Sequence LengthMass (Da)Tools
O31646-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 6848958CF5545064

FASTA31536,003
        10         20         30         40         50         60 
MTTEPLFFKP VFKERIWGGT ALADFGYTIP SQRTGECWAF AAHQNGQSVV QNGMYKGFTL 

        70         80         90        100        110        120 
SELWEHHRHL FGQLEGDRFP LLTKILDADQ DLSVQVHPND EYANIHENGE LGKTECWYII 

       130        140        150        160        170        180 
DCQKDAEIIY GHNATTKEEL TTMIERGEWD ELLRRVKVKP GDFFYVPSGT VHAIGKGILA 

       190        200        210        220        230        240 
LETQQNSDTT YRLYDYDRKD AEGKLRELHL KKSIEVIEVP SIPERHTVHH EQIEDLLTTT 

       250        260        270        280        290        300 
LIECAYFSVG KWNLSGSASL KQQKPFLLIS VIEGEGRMIS GEYVYPFKKG DHMLLPYGLG 

       310 
EFKLEGYAEC IVSHL

« Hide

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References

« Hide 'large scale' references
[1]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[2]"Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis."
Reizer J., Bachem S., Reizer A., Arnaud M., Saier M.H. Jr., Stuelke J.
Microbiology 145:3419-3429(1999) [PubMed: 10627040] [Abstract]
Cited for: GENE NAME.
Strain: 168.
[3]"Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis."
Turner M.S., Helmann J.D.
J. Bacteriol. 182:5202-5210(2000) [PubMed: 10960106] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: 168.

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Cross-references

Sequence databases

AL009126 Genomic DNA. Translation: CAB13059.1.
PIRH69848.
RefSeqNP_389084.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID939393.
GenomeReviewsGene locus BSU12020 in contig AL009126_GR.
KEGGbsu:BSU12020.
NMPDRfig|224308.1.peg.1203.

Organism-specific databases

SubtiListBG13177. manA. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMO31646.
OMAILDADQD.

Enzyme and pathway databases

BioCycBSUB224308:BSU1203-MON.

Family and domain databases

InterProIPR001250. Man6P_Isoase-1.
IPR014628. Man6P_isomerase_Firm_short.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
Gene3DG3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
PfamPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFPIRSF036894. PMI_Firm_short. 1 hit.
TIGRFAMsTIGR00218. manA. 1 hit.
PROSITEPS00965. PMI_I_1. False negative.
PS00966. PMI_I_2. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMANA1_BACSU
AccessionPrimary (citable) accession number: O31646
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 1, 1998
Last modified: November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents