Reviewed, UniProtKB/Swiss-Prot O31644 (MANR_BACSU)

Names and origin

Protein names

Recommended name:
    Probable transcriptional regulator manR
Including the following 2 domains:
    1- Recommended name:
            Putative phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            Putative PTS system EIIB component
    2- Recommended name:
            Putative phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            Putative PTS system EIIA component

Gene names

Name:	manR
Ordered Locus Names:	BSU12000

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May regulate the expression of the mannose operon.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Domain	The PTS EIIB type-2 domain may serve a regulatory function, through its phosphorylation activity. The PTS EIIA type-2 domain may serve a regulatory function, through its phosphorylation activity.
Miscellaneous	Might be activated by phosphorylation of certain histidyl residues by HPr By similarity.
Sequence similarities	Belongs to the transcriptional antiterminator bglG family. Contains 2 PRD domains. Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Repeat
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Probable transcriptional regulator manR		PRO_0000376080
Regions
	Domain	187 – 292	106	PRD 1
	Domain	297 – 404	108	PRD 2
	Domain	409 – 500	92	PTS EIIB type-2
	Domain	510 – 648	139	PTS EIIA type-2
Amino acid modifications
	Modified residue	222	1	Phosphohistidine Potential
	Modified residue	281	1	Phosphohistidine Potential
	Modified residue	334	1	Phosphohistidine Potential
	Modified residue	393	1	Phosphohistidine Potential
	Modified residue	415	1	Phosphocysteine; by EIIA By similarity
	Modified residue	570	1	Phosphohistidine; by HPr By similarity

Sequences

	Sequence		Length	Mass (Da)
	O31644-1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: E987358049EBA550	FASTA	648	75,351
	10 20 30 40 50 60 MEYINTRQKE ILYLLLSEPD DYLVVQDFAD RVQCSEKTIR NDLKVIEDYL NEHSHAQLIR 70 80 90 100 110 120 KPGLGVYLHI EEQERTWLSQ QLHTEHFSSR QRSDKERMLH IAYDLLMNPK PVSAKDIAAR 130 140 150 160 170 180 HFVNRSSIKK DLYAVEEWLK RFDLTLVSRQ RLGLKVEGNE RNKRKALARI SDLIHNTAFT 190 200 210 220 230 240 SQFIKSKFLH YEVDFVTKEI KSLQKKHSLY FTDETFESLL LHTLLMVRRI KMKQPISLSP 250 260 270 280 290 300 KEMAAVKKKK EYQWTFACLQ RLEPVFAIRF PEEEAVYLTL HILGGKVRYP LQTEENLENA 310 320 330 340 350 360 VLPKVVGHLI NRVSELKMMD FHKDQDLING LNIHLNTVLQ RLSYDLSVAN PMLNDIKKMY 370 380 390 400 410 420 PYLFHLIIDV LEDINQTFDL HIPEEEAAYL TLHFQAAIER MQGSSETHKK AVIVCHMGIG 430 440 450 460 470 480 MSQLLRTKIE RKYHQIAVMA CIAKADLKDY IKKHEDIDLV ISTIALENIT VPHIVVSPLL 490 500 510 520 530 540 EPGEEKKLSA FIRQLGESHR QKQKTFQMLN NTTPFLVFLQ QEAEHRYKLI EQLATALFEK 550 560 570 580 590 600 GYVDKDYAVH AVMREKMSAT NIGSGIAIPH ANAKFIKQSA IAIATLKEPL EWGNEKVSLV 610 620 630 640 FMLAVKHEDQ TMTKQLFSEL SYLSEQPAFV QKLTKETNVM TFLSHLDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 106.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB13057.2.
RefSeq	NP_389082.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939394.
GenomeReviews	Gene locus BSU12000 in contig AL009126_GR.
KEGG	bsu:BSU12000.
NMPDR	fig|224308.1.peg.1201.
Organism-specific databases
SubtiList	BG13175. manR. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O31644.
OMA	RTKIERK.
Family and domain databases
InterPro	IPR013196. HTH_11. IPR011608. PRD. IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR001550. Transcrpt_antitermin_CS. [Graphical view]
Gene3D	G3DSA:1.10.1790.10. PRD. 2 hits. G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF08279. HTH_11. 1 hit. PF00874. PRD. 2 hits. PF00359. PTS_EIIA_2. 1 hit. [Graphical view]
PROSITE	PS00654. PRD_1. False negative. PS51372. PRD_2. 2 hits. PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANR_BACSU

Accession

Primary (citable) accession number: O31644

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 5, 2009
Last modified:	November 3, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)